US4879219A
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Immunoassay utilizing monoclonal high affinity IgM antibodies
Assignee: General Hospital Corp
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Filing: 1980-09-19
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Granted: 1989-11-07
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Expires: 2000-09-19
Abstract
Abstract Hybridomally produced monoclonal IgM antibodies having high affinity are useful for the immunoassay and purification of viral antigens.
Inventors
- 👤 Jack R. Wands
- 👤 Vincent R. Zurawski, Jr.
- 👤 Hubert J. P. Schoemaker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:43
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C07K16/00; C07K16/08; C07K16/081; C07K16/082; C07K16/10; A; A61; A61K; A61K39/00; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/82; Y10S530/00; Y10S530/82; Y10S530/826; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
72 words
An immunoassay method utilizing an antibody to assay for a substance comprising hepatitis B-surface antigen (HBsAg) determinants which comprises the steps of: contacting a test sample containing said substance comprising HBsAg determinants with said antibody; and determining the presence of said substance in said sample; wherein said antibody is a monoclonal high affinity IgM antibody having a binding affinity constant for said HBsAg determinants of at least 10 9 M -1 .
Claim 2
Dependent Claim
72 words
An immunoassay method utilizing an antibody to assay for a substance comprising hepatitis B-surface antigen (HBsAg) determinants which comprises the steps of: contacting a test sample containing said substance comprising HBsAg determinants with said antibody; and determining the presence of said substance in said sample; wherein said antibody is a monoclonal high affinity IgM antibody having a binding affinity constant for said HBsAg determinants of at least 10 9 M -1 .
Claim 3
Dependent Claim
17 words
The assay method of claim 1 wherein said constant is at least 10 10 M -1 .
Claim 4
Dependent Claim
22 words
The assay method of claim 1 which is selected from the group consisting of radioimmunoassay, enzyme immunoassay, agglutination immunoassay, and fluorescence immunoassay.
Claim 5
Dependent Claim
9 words
The assay method of claim 3 which is radioimmunoassay.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein said HBsAg determinants are present in Hepatitis B virus.
Claim 7
Dependent Claim
20 words
The assay method of claim 1 wherein said monoclonal high affinity IgM antibodies are coupled to an insoluble solid phase.
Claim 8
Dependent Claim
40 words
Monoclonal high affinity IgM antibodies immunoreactive with HBsAg determinants wherein said antibodies are coupled to an insoluble solid phase, and wherein the binding affinity constant of said antibodies for said HBsAg determinants is at least 10 9 M -1 .
Claim 9
Dependent Claim
40 words
Monoclonal high affinity IgM antibodies immunoreactive with HBsAg determinants wherein said antibodies are coupled to an insoluble solid phase, and wherein the binding affinity constant of said antibodies for said HBsAg determinants is at least 10 9 M -1 .
Claim 10
Dependent Claim
13 words
The antibodies of claim 7 which are immunoreactive with hepatitis B surface antigen.
Claim 11
Dependent Claim
11 words
The antibodies of claim 8 wherein said label is a radiolabel.
References Cited (Backward Citations)
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