US4172124A
Expired / Lapsed
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Method of producing tumor antibodies
Assignee: The Wistar Institute
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Filing: 1978-04-28
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Granted: 1979-10-23
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Expires: 1998-04-28
Abstract
Abstract Antibodies demonstrating a specificity for malignant tumors are produced by somatic cell hybrids between hypoxanthine phosphoribosyltransferase deficient myeloma cells and spleen or lymph cells derived from an animal previously primed with tumor cells.
Inventors
- 👤 Hilary Koprowski
- 👤 Carlo M. Croce
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:44
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; A; A61; A61P; A61P35/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
45 words
A method of producing malignant tumor antibodies comprising immunizing an animal with tumor cells, forming fused cell hybrids between antibody producing cells from said animal and myeloma cells, cloning said hybrids and selecting clones which produce antibodies that demonstrate a specificity for said tumor cells.
Claim 2
Independent Claim
45 words
A method of producing malignant tumor antibodies comprising immunizing an animal with tumor cells, forming fused cell hybrids between antibody producing cells from said animal and myeloma cells, cloning said hybrids and selecting clones which produce antibodies that demonstrate a specificity for said tumor cells.
Claim 3
Dependent Claim
25 words
A method according to claim 1 wherein the tumor antibody producing cell is selected from the group consisting of spleen cells and lymph node cells.
Claim 4
Dependent Claim
15 words
A method according to claim 2 wherein the antibody producing cell is a spleen cell.
Claim 5
Dependent Claim
19 words
A method according to claim 1 wherein said animal is selected from the group consisting of mice and rats.
Claim 6
Dependent Claim
15 words
A method according to claim 1 wherein the animal is immunized with human cancer cells.
Claim 7
Dependent Claim
12 words
A method according to claim 4 wherein the cancer is a melanoma.
Claim 8
Dependent Claim
13 words
A method according to claim 4 wherein the cancer is a colorectal carcinoma.
Claim 9
Dependent Claim
16 words
A method according to claim 1 wherein the hybrid is cultured in a medium containing hypoxanthine-aminopterin-thymidine.
Claim 10
Dependent Claim
20 words
A method according to claim 1 wherein both the antibody producing cells and the myeloma cells are derived from mice.
Claim 11
Dependent Claim
30 words
The process of claim 1 wherein said hybrid is introduced into an animal selected from the group consisting of a histocompatible animal and athymic nude mice and cultured in vivo.
Claim 12
Dependent Claim
32 words
A method according to claim 1 wherein a mouse is immunized with tumor cells and a fused cell hybrid is formed between the spleen cells of said mouse and mouse myeloma cells.
Claim 13
Dependent Claim
14 words
The method according to claim 11 wherein said immunized mouse is a BALB/c mouse.
Claim 14
Dependent Claim
13 words
The method according to claim 11 wherein said tumor cells are melanoma cells.
Claim 15
Dependent Claim
13 words
The method according to claim 11 wherein said tumor cells are colo-rectal carcinoma.
Claim 16
Dependent Claim
16 words
The method according to claim 10 wherein the hybrid is cultured in a medium containing hypoxanthine-aminopterin-thymidene.
Claim 17
Dependent Claim
30 words
The process of claim 11 wherein said hybrid is introduced into an animal selected from the group consisting of a histocompatible animal and athymic nude mice and cultured in vivo.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-22 | 1983-04-23 | 1983-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-22 | 1987-04-23 | 1987-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-22 | 1991-04-23 | 1991-10-23 | $7700.00 | Window Closed / Lapsed |