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Patent US4834854A successfully refreshed from Google Patents.
US4834854A
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US
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Method of making an electrophoresis medium
Assignee: Fujifilm Holdings Corp
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Filing: 1988-08-19
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Granted: 1989-05-30
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Expires: 2008-08-19
Abstract
Abstract A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent comprises the steps of mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator. Viscosity values of the two kinds of the aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to the mixing are adjusted to be substantially equal to each other.
Inventors
- 👤 Mitsuru Sugihara
- 👤 Mineo Suyefuji
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:32
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
147 words
A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent and containing a compound having at least one carbamoyl group in its molecule as a denaturing agent by mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator, wherein the improvement comprises the step of adjusting so that viscosity values of the two kinds of said aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to said mixing are substantially equal to each other.
Claim 2
Independent Claim
147 words
A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent and containing a compound having at least one carbamoyl group in its molecule as a denaturing agent by mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator, wherein the improvement comprises the step of adjusting so that viscosity values of the two kinds of said aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to said mixing are substantially equal to each other.
Claim 3
Dependent Claim
61 words
A method as defined in claim 1 wherein a difference between the viscosity values of the two kinds of said aqueous solutions for gel formation is within the range of approximately 12% above and below a mean value of the viscosity values of the two kinds of the aqueous solutions for gel formation which are to be mixed with each other.
Claim 4
Dependent Claim
22 words
A method as defined in claim 1 wherein agarose is contained in at least one of said aqueous solutions for gel formation.
Claim 5
Dependent Claim
24 words
A method as defined in claim 1 wherein a water-soluble polymer is contained in at least one of said aqueous solutions for gel formation.
Claim 6
Dependent Claim
13 words
A method as defined in claim 1 wherein said denaturing agent is urea.
Claim 7
Dependent Claim
28 words
A method as defined in claim 1 wherein said electrophoresis medium is provided as a layer-shaped electrophoresis medium membrane between a planar support and a planar cover sheet.
Claim 8
Dependent Claim
21 words
A method as defined in claim 6 wherein said support and said cover sheet are sheet-shaped materials formed of polyethylene terephthalate.
Claim 9
Dependent Claim
26 words
A method as defined in claim 1, 2 or 3 wherein said electrophoresis medium is provided as a pillar-shaped electrophoresis medium inside of a tube-shaped support.
Claim 10
Independent Claim
130 words
A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent by mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator, wherein the improvement comprises the step of adjusting so that viscosity values of the two kinds of said aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to said mixing are substantially equal to each other.
Claim 11
Independent Claim
130 words
A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent by mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator, wherein the improvement comprises the step of adjusting so that viscosity values of the two kinds of said aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to said mixing are substantially equal to each other.
Claim 12
Dependent Claim
61 words
A method as defined in claim 9 wherein a difference between the viscosity values of the two kinds of said aqueous solutions for gel formation is within the range of approximately 12% above and below a mean value of the viscosity values of the two kinds of the aqueous solutions for gel formation which are to be mixed with each other.
Claim 13
Dependent Claim
24 words
A method as defined in claim 9 wherein a water-soluble polymer is contained in at least one of said aqueous solutions for gel formation.
Claim 14
Dependent Claim
30 words
A method as defined in claim 9 wherein an anionic surface active agent as a denaturing agent is contained in at least one of said aqueous solutions for gel formation.
Claim 15
Dependent Claim
17 words
A method as defined in claim 12 wherein said anionic surface active agent is an alkyl sulfate.
Claim 16
Dependent Claim
22 words
A method as defined in claim 13 wherein said alkyl sulfate is a sodium salt or a potassium salt of dodecylsulfuric acid.
Claim 17
Dependent Claim
28 words
A method as defined in claim 9 wherein said electrophoresis medium is provided as a layer-shaped electrophoresis medium membrane between a planar support and a planar cover sheet.
Claim 18
Dependent Claim
21 words
A method as defined in claim 15 wherein said support and said cover sheet are sheet-shaped materials formed of polyethylene terephthalate.
Claim 19
Independent Claim
27 words
A method as defined in any of claims 9 to 14 wherein said electrophoresis medium is provided as a pillar-shaped electrophoresis medium inside of a tube-shaped support.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-29 | 1992-11-28 | 1993-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-29 | 1996-11-28 | 1997-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-29 | 2000-11-28 | 2001-05-30 | $7700.00 | Window Closed / Lapsed |