Patent US4766069A successfully refreshed from Google Patents.
US4766069A
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Recombinant DNA which codes for interleukin-1 B
Assignee: MIT
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Filing: 1987-01-08
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Granted: 1988-08-23
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Expires: 2007-01-08
Abstract
Abstract The subject invention concerns a nucleic acid comprising a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Specifically, the subject invention comprises the cloning of a cDNA synthesized by reverse transcription of poly(A)RNA isolated from adherent human monocytes stimulated with bacterial endotoxin. Human IL-1 is useful to induce the production of IL-2 by activated T-cells; it also acts on B-cells and NK-cells.
Inventors
- 👤 Philip E. Auron
- 👤 Charles A. Dinarello
- 👤 Andrew C. Webb
- 👤 Alexander Rich
- 👤 Sheldon M. Wolff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:38
CPC Classifications
C; C12; C12P; C12P21/00; C12P21/02; A; A61; A61P; A61P43/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/545; C07K14/81; C07K14/8107; C07K14/811; C07K14/8121; C07K14/8132; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/141
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
16 words
A recombinant DNA cloning vehicle comprising a DNA sequence comprising the human IL-1 gene DNA sequence.
Claim 2
Dependent Claim
16 words
A recombinant DNA cloning vehicle comprising a DNA sequence comprising the human IL-1 gene DNA sequence.
Claim 3
Dependent Claim
19 words
The cloning vehicle of claim 1 wherein the DNA sequence comprises DNA coding for the amino acid sequence: ##STR26##
Claim 4
Dependent Claim
23 words
The cloning vehicle of claim 1 comprising the DNA sequence: ##STR27## and equivalent nucleotide sequences thereof coding for the amino acid sequence: ##STR28##
Claim 5
Dependent Claim
11 words
Essentially pure DNA coding for human IL-1B having the sequence: ##STR29##
Claim 6
Dependent Claim
11 words
Essentially pure DNA coding for human IL-1B having the sequence: ##STR29##
Claim 7
Dependent Claim
10 words
A microorganism transformed by the cloning vehicle of claim 1.
Claim 8
Dependent Claim
10 words
A microorganism transformed by the cloning vehicle of claim 2.
Claim 9
Dependent Claim
14 words
A mammalian tissue culture cell line transfected by the cloning vehicle of claim 1.
Claim 10
Dependent Claim
14 words
A mammalian tissue culture cell line transfected by the cloning vehicle of claim 2.
Claim 11
Dependent Claim
3 words
Recombinant plasmid pcD-415.
Claim 12
Dependent Claim
3 words
Recombinant plasmid pcD-415.
Claim 13
Dependent Claim
9 words
Bacteria transformed by the recombinant plasmid of claim 9.
Claim 14
Dependent Claim
6 words
The microorganism E. coli HB101 (pcD-415).
Claim 15
Dependent Claim
6 words
The microorganism E. coli HB101 (pcD-415).
Claim 16
Dependent Claim
24 words
A process for preparing human IL-1B which comprises culturing a microbe hosting a cloning vehicle comprising DNA encoding human IL-1B and recovering human IL-1B.
Claim 17
Dependent Claim
24 words
A process for preparing human IL-1B which comprises culturing a microbe hosting a cloning vehicle comprising DNA encoding human IL-1B and recovering human IL-1B.
Claim 18
Dependent Claim
13 words
A process, according to claim 12, wherein said cloning vehicle is plasmid pcD-415.
Claim 19
Dependent Claim
11 words
Essentially pure DNA coding for human IL-1B having the sequence: ##STR30##
Claim 20
Dependent Claim
11 words
Essentially pure DNA coding for human IL-1B having the sequence: ##STR30##
Claim 21
Dependent Claim
19 words
A recombinant DNA cloning vehicle comprising a DNA sequence comprising cDNA coding for human IL-1b having the sequence: ##STR31##
Claim 22
Dependent Claim
19 words
A recombinant DNA cloning vehicle comprising a DNA sequence comprising cDNA coding for human IL-1b having the sequence: ##STR31##
Claim 23
Dependent Claim
19 words
The cloning vehicle of claim 15 wherein the DNA sequence comprises DNA coding for the amino acid sequence: ##STR32##
Claim 24
Dependent Claim
23 words
The cloning vehicle of claim 15 comprising the DNA sequence: ##STR33## and equivalent nucleotide sequences thereof coding for the amino acid sequence: ##STR34##
Claim 25
Dependent Claim
10 words
A microorganism transformed by the cloning vehicle of claim 15.
Claim 26
Dependent Claim
14 words
A mammalian tissue culture cell line transfected by the cloning vehicle of claim 15.
Claim 27
Dependent Claim
10 words
A microorganism transformed by the cloning vehicle of claim 16.
Claim 28
Dependent Claim
14 words
A mammalian tissue culture cell line transfected by the cloning vehicle of claim 16.
Claim 29
Dependent Claim
36 words
A process for preparing a human IL-1B peptide which comprises culturing a microbe hosting a cloning vehicle comprising DNA having the following sequence: ##STR35## and equivalent nucleotide sequences thereof coding for the amino acid sequence: ##STR36##
Claim 30
Dependent Claim
36 words
A process for preparing a human IL-1B peptide which comprises culturing a microbe hosting a cloning vehicle comprising DNA having the following sequence: ##STR35## and equivalent nucleotide sequences thereof coding for the amino acid sequence: ##STR36##
Claim 31
Dependent Claim
3 words
Recombinant plasmid pcD-1218.
Claim 32
Dependent Claim
3 words
Recombinant plasmid pcD-1218.
Claim 33
Dependent Claim
3 words
Recombinant plasmid pA-26.
Claim 34
Dependent Claim
3 words
Recombinant plasmid pA-26.
References Cited (Backward Citations)
GB2063882A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-23 | 1992-02-22 | 1992-08-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-23 | 1996-02-22 | 1996-08-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-23 | 2000-02-22 | 2000-08-23 | $7700.00 | Window Closed / Lapsed |