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US4757006A
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Human factor VIII:C gene and recombinant methods for production
Assignee: Genetics Institute LLC
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Filing: 1983-10-28
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Granted: 1988-07-12
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Expires: 2003-10-28
Abstract
Abstract The protein having factor VIII:C procoagulant activity has been produced by culturing a cell transformed with a recombinant expression vector encoding the gene for that activity.
Inventors
- 👤 John J. Toole, Jr.
- 👤 Edward Fritsch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:53
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/10
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
18 words
An isolated recombinant vector containing DNA coding for human factor VIII:C, comprising a polydeoxyribonucleotide having the sequence: ##STR2##
Claim 2
Dependent Claim
18 words
An isolated recombinant vector containing DNA coding for human factor VIII:C, comprising a polydeoxyribonucleotide having the sequence: ##STR2##
Claim 3
Dependent Claim
14 words
The recombinant vector DNA of claim 1, wherein said DNA is human genomic DNA.
Claim 4
Dependent Claim
24 words
The vector of claim 1, wherein the DNA coding for human factor VIII:C is linked directly or indirectly to DNA from a non-human source.
Claim 5
Dependent Claim
25 words
A non-human recombinant expression vector for human factor VIII:C comprising a DNA segment having the sequence: ##STR3## operatively linked to a heterologous regulatory control sequence.
Claim 6
Dependent Claim
25 words
A non-human recombinant expression vector for human factor VIII:C comprising a DNA segment having the sequence: ##STR3## operatively linked to a heterologous regulatory control sequence.
Claim 7
Dependent Claim
13 words
A transformed non-human mammalian cell line containing the expression vector of claim 4.
Claim 8
Dependent Claim
37 words
A screening agent for identifying deoxyribonucleotide sequences and ribonucleotide sequences which encode for at least a portion of the human gene factor VIII:C, consisting essentially of a deoxyribonucleotide having the following sequence or its inverse complement: ##STR4##
Claim 9
Dependent Claim
37 words
A screening agent for identifying deoxyribonucleotide sequences and ribonucleotide sequences which encode for at least a portion of the human gene factor VIII:C, consisting essentially of a deoxyribonucleotide having the following sequence or its inverse complement: ##STR4##
Claim 10
Independent Claim
71 words
A method of producing a protein having factor VIII:c procoagulant activity, comprising the steps of: (1) culturing an isolated cell transformed with double stranded DNA which DNA (a) has been operatively linked to a heterologous regulatory control sequence, (b) hybridizes under stringent conditions to the following nucleotide sequence: ##STR5## and (c) encodes a protein which upon expression exhibits human factor VIII:c procoagulant activity, and (2) recovering said protein from the culture.
Claim 11
Independent Claim
71 words
A method of producing a protein having factor VIII:c procoagulant activity, comprising the steps of: (1) culturing an isolated cell transformed with double stranded DNA which DNA (a) has been operatively linked to a heterologous regulatory control sequence, (b) hybridizes under stringent conditions to the following nucleotide sequence: ##STR5## and (c) encodes a protein which upon expression exhibits human factor VIII:c procoagulant activity, and (2) recovering said protein from the culture.
Claim 12
Dependent Claim
26 words
A transformed non-human mammalian cell containing a gene encoding human factor VIII:c in operable association with a heterlogous regulatory control sequence in an expression vector therefor.
Claim 13
Dependent Claim
26 words
A transformed non-human mammalian cell containing a gene encoding human factor VIII:c in operable association with a heterlogous regulatory control sequence in an expression vector therefor.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-12 | 1992-01-11 | 1992-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-12 | 1996-01-11 | 1996-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-12 | 2000-01-11 | 2000-07-12 | $7700.00 | Window Closed / Lapsed |