US4752578A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Collagenase inducing factor
Assignee: Monsanto Co
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Filing: 1986-04-11
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Granted: 1988-06-21
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Expires: 2006-04-11
Abstract
Abstract A method for the production of collagenase inducing factor is disclosed which comprises growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture medium at 35 DEG -38 DEG C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.
Inventors
- 👤 William M. Moore
- 👤 Curtis A. Spilburg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:50
CPC Classifications
C; C12; C12P; C12P39/00; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4702; C07K14/4705
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
49 words
Method for the production of collagenase inducing factor that stimulates synthesis of synovial cell collagenase and prostaglandin E 2 comprising growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture media at 35°-38° C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.
Claim 2
Dependent Claim
49 words
Method for the production of collagenase inducing factor that stimulates synthesis of synovial cell collagenase and prostaglandin E 2 comprising growing the human liver adenocarcinoma cell line SK-HEP-1 in nutrient culture media at 35°-38° C. and recovering the resulting collagenase inducing factor from the spent cells or conditioned medium.
Claim 3
Dependent Claim
52 words
The method of claim 1 in which the collagenase inducing factor is recovered from the spent cells by freezing and thawing said cells suspended in low ionic buffer followed by subjecting the resulting cell lysate to the stepwise sequence of chromatofocusing with Polybuffer exchanger, Phenyl-Sepharose chromatography, and Bio-Gel P-30 gel filtration chromatography.
Claim 4
Dependent Claim
37 words
The method of claim 1 in which the collagenase inducing factor is recovered from the conditioned medium by the stepwise sequence of ammonium sulfate fractionation, Affi-Gel Blue chromatography, Bio-Gel HTP chromatography, and Sephadex G-75 gel filtration chromatography.
References Cited (Backward Citations)
US4209587A
Cited
US4289854A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-06-21 | 1991-12-21 | 1992-06-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-06-21 | 1995-12-21 | 1996-06-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-06-21 | 1999-12-21 | 2000-06-21 | $7700.00 | Window Closed / Lapsed |