US4289854A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture method and apparatus
Assignee: Monsanto Co
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Filing: 1980-06-20
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Granted: 1981-09-15
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Expires: 2000-06-20
Abstract
Abstract A cell culture system and apparatus is provided for the agitated suspension culturing of mammalian cells in a flask assembly in which the culture medium and cells are gently agitated by the slow rotation of agitator means suspended downwardly in the culture medium and having at least one flexible sheet with a relatively large surface area which is positioned on the agitator in a manner to allow it to billow like a sail as liquid spills from its trailing edge during said rotation of the agitator means.
Inventors
- 👤 William R. Tolbert
- 👤 Mary M. Hitt
- 👤 Joseph Feder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:20
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/58; B; B01; B01F; B01F27/00; B01F27/05; B01F27/051; B01F27/054; B01F33/00; B01F33/45; B01F33/453; C12M25/00; C12M25/16; C12M27/00; C12M27/02; C12M33/00; C12M33/22
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
80 words
Apparatus for the suspension culturing of cells comprising (A) a hollow flask having an opening at the top, (B) shaft means within said flask and supported downwardly from said top opening, and (C) a rotatable agitator means supported downwardly from said shaft means, said agitator means having supported thereon at least one flexible sheet, said flexible sheet being thus supported at upper and lower positions of the sheet to permit billowing of said sheet during rotation of the agitator means.
Claim 2
Dependent Claim
80 words
Apparatus for the suspension culturing of cells comprising (A) a hollow flask having an opening at the top, (B) shaft means within said flask and supported downwardly from said top opening, and (C) a rotatable agitator means supported downwardly from said shaft means, said agitator means having supported thereon at least one flexible sheet, said flexible sheet being thus supported at upper and lower positions of the sheet to permit billowing of said sheet during rotation of the agitator means.
Claim 3
Dependent Claim
38 words
Apparatus of claim 1 in which the flexible sheet has a surface area on one side which ranges from about 0.25 to about 1.0 times the available traversable cross-sectional area of the culture fluid-containing portion of said flask.
Claim 4
Dependent Claim
40 words
Apparatus of claim 1 in which the agitator means comprises a rotatable shaft and a plurality of flexible sheets, each said sheet being substantially equidistantly spaced apart from each other sheet circumferentially about the vertical axis of said rotatable shaft.
Claim 5
Dependent Claim
40 words
Apparatus of claim 3 in which each flexible sheet is supported at said lower position of the agitator means ahead of said upper position relative to the direction of rotation whereby said flexible sheet is provided with a scoop-like configuration.
Claim 6
Dependent Claim
40 words
Apparatus of claim 3 in which each flexible sheet is supported at said upper position of the agitator means ahead of said lower position relative to the direction of rotation whereby said flexible sheet is provided with a concave configuration.
Claim 7
Dependent Claim
65 words
Apparatus of claim 3 in which said shaft has a hollow bore and a side opening through an outer wall, and in which said flexible sheets are pivotably supported at their lower ends on said shaft with pivot means in combination with line means downwardly disposed from the top of said shaft through said bore and said side opening and secured to said pivot means.
Claim 8
Dependent Claim
116 words
Apparatus of claim 1 in combination with a stationary filter unit supported downwardly from said shaft means in which said shaft means has a hollow interior in fluid communication with said top opening of said flask, in which said filter unit has a fluid collection cavity in direct fluid communication with the interior of said shaft means and the top of said flask and a porous peripheral surface having a pore size smaller than the cells to be cultured or the carrier particles on which said cells are attached but sufficiently large to permit permeation of fluid into said fluid collection cavity, and in which said rotatable agitator means is concentrically disposed about said filter unit.
Claim 9
Dependent Claim
28 words
In the method of culturing animal cells in agitated liquid suspension of nutrient culture medium, the improvement comprising carrying out the agitation with the apparatus of claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-14 | 1985-03-16 | 1985-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-14 | 1989-03-16 | 1989-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-14 | 1993-03-16 | 1993-09-15 | $7700.00 | Window Closed / Lapsed |