Patent US3873817A successfully refreshed from Google Patents.
US4663277A
Expired / Lapsed
US
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Virus detection method and materials
Assignee: Profile Diagnostic Sciences Inc
•
Filing: 1983-05-20
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Granted: 1987-05-05
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Expires: 2003-05-20
Abstract
Abstract Viruses are detected by means of an immunoassay method in which an extended solid phase coated with antiviral antibody is employed to bind and remove virions from a specimen by forming an immuno-complex with antigens of said virions, a mobile solid phase comprising a dispersion of microspheres coated with the antiviral antibody is used to bind said microspheres to antigens associated with said immuno-complex, and the presence of bound microspheres is detected. The detection sensitivity is amplified by the ability to more readily detect the microspheres, which may be dyed or labelled. The extended solid phase advantageously may be in the form of a dipstick which can be easily contacted with the specimen. A virus detection kit provides the extended solid phase and mobile solid phases, each coated with antiviral antibodies.
Inventors
- 👤 Chia-Gee Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54306; G01N33/54313; G01N33/54353; G01N33/54366; G01N33/54386; G01N33/551; G01N33/553; G01N33/569; G01N33/56983; G01N33/58; G01N33/585; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/962; Y10S435/968; Y10S435/97; Y10S435/973; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/808; Y10S436/81; Y10S436/82; Y10S436/824; Y10S436/825
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
136 words
A method for detection of viruses in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiviral antibody (Ab v ) which is capable of forming immuno-complexes with antigens characteristic of the viruses to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres smaller than 0.1 μm, said microspheres being labelled with metal elements and having conjugated thereto said Ab v which enables the binding of said microspheres to said immuno-complexes; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, thereby detecting or determining the presence of viruses in said specimen.
Claim 2
Independent Claim
136 words
A method for detection of viruses in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiviral antibody (Ab v ) which is capable of forming immuno-complexes with antigens characteristic of the viruses to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres smaller than 0.1 μm, said microspheres being labelled with metal elements and having conjugated thereto said Ab v which enables the binding of said microspheres to said immuno-complexes; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, thereby detecting or determining the presence of viruses in said specimen.
Claim 3
Dependent Claim
31 words
A method according to claim 1, wherein before contacting with the solid phase support, the specimen is contacted with a polystyrene latex surface coated with gamma immunoglobulin to remove non-specific agglutinators.
Claim 4
Dependent Claim
12 words
A method according to claim 1, wherein the Ab v is monoclonal.
Claim 5
Dependent Claim
27 words
A method according to claim 1, wherein the solid phase support is both separated from the specimen and also separated from the mobile solid phase by washing.
Claim 6
Dependent Claim
72 words
A method according to claim 1, wherein antispecies antibody (Ab s ) is covalently bound to the solid phase support and is bound to the mobile solid phase, and Ab v which forms an immuno-complex with said Ab s is coupled therewith, whereby there is conjugated to said solid phase support and to said mobile solid phase Ab v which is capable of forming immuno-complexes with antigens of viruses to be detected.
Claim 7
Dependent Claim
12 words
A method according to claim 5, wherein the Ab v is monoclonal.
Claim 8
Dependent Claim
22 words
A method according to claim 1, wherein the solid phase support forms at least part of a dipstick, syringe, tube or container.
Claim 9
Dependent Claim
32 words
A method according to claim 1, wherein the solid phase support forms at least part of a dipstick, said solid phase support being included at least at an end of said dipstick.
Claim 10
Dependent Claim
24 words
A method according to claim 8, wherein the Ab v conjugated on the solid phase support is concentrated at an end of the dipstick.
Claim 11
Dependent Claim
27 words
A method according to claim 7, wherein the solid phase support comprises an acrylic resin, or comprises an inorganic material treated for covalent coupling with antibody protein.
Claim 12
Dependent Claim
61 words
A method according to claim 1, wherein a plurality of different Ab v proteins capable of forming complexes with corresponding antigens of different types of viruses are conjugated to the solid phase support and are conjugated to the mobile solid phase, whereby the presence of one or more of a plurality of different types of viruses in the specimen is detected.
Claim 13
Dependent Claim
191 words
A virus detection kit, which comprises as individual components: (a) a solid phase support having conjugated thereon antiviral antibody (Ab v ) capable of forming immuno-complexes with antigens characteristic of the viruses to be detected, said antiviral antibody consisting of a plurality of different Ab v proteins capable of forming complexes with corresponding antigens of different types of viruses; and (b) a mobile solid phase consisting of dispersed microspheres smaller than 0.1 μm, said microspheres being labelled with a plurality of metal elements which can be detected by X-ray fluorescence and having said different Ab v proteins conjugated thereto, said mobile solid phase being either in the form of a mixture of different types of microspheres, each type having conjugated thereto a different Ab v protein, the different Ab v proteins being capable of forming complexes with corresponding antigens of different viruses, or in the form of separate batches of microspheres, each batch having conjugated thereto a different Ab v protein, said different Ab v proteins being capable of forming complexes with corresponding antigens of different viruses; wherein the components are present in amounts sufficient to perform the virus detection assay.
Claim 14
Dependent Claim
191 words
A virus detection kit, which comprises as individual components: (a) a solid phase support having conjugated thereon antiviral antibody (Ab v ) capable of forming immuno-complexes with antigens characteristic of the viruses to be detected, said antiviral antibody consisting of a plurality of different Ab v proteins capable of forming complexes with corresponding antigens of different types of viruses; and (b) a mobile solid phase consisting of dispersed microspheres smaller than 0.1 μm, said microspheres being labelled with a plurality of metal elements which can be detected by X-ray fluorescence and having said different Ab v proteins conjugated thereto, said mobile solid phase being either in the form of a mixture of different types of microspheres, each type having conjugated thereto a different Ab v protein, the different Ab v proteins being capable of forming complexes with corresponding antigens of different viruses, or in the form of separate batches of microspheres, each batch having conjugated thereto a different Ab v protein, said different Ab v proteins being capable of forming complexes with corresponding antigens of different viruses; wherein the components are present in amounts sufficient to perform the virus detection assay.
Claim 15
Dependent Claim
25 words
A kit according to claim 12, further including as an individual component: (c) a latex solid phase capable of removing non-specific agglutinators from a specimen.
Claim 16
Dependent Claim
15 words
A kit according to claim 13, wherein said latex is polystyrene coated with gamma immuno-globulin.
Claim 17
Dependent Claim
76 words
A kit according to claim 12, wherein the solid phase support component (a) and the mobile solid phase component (b) each have antispecies antibody (Ab s ) covalently bound thereto, and Ab v which forms an immuno-complex with said Ab s coupled therewith, whereby said solid phase support component (a) and said mobile solid phase component (b) each have conjugated thereto Ab v which is capable of forming immuno-complexes with antigens of viruses to be detected.
Claim 18
Dependent Claim
24 words
A kit according to claim 12, wherein the solid phase support component (a) forms at least part of a dipstick, syringe, tube or container.
Claim 19
Dependent Claim
32 words
A kit according to claim 12, wherein the solid phase support forms at least part of a dipstick, said solid phase support being included at least at an end of said dipstick.
Claim 20
Dependent Claim
24 words
A kit according to claim 17, wherein the Ab v conjugated on the solid phase support is concentrated at an end of the dipstick.
Claim 21
Dependent Claim
27 words
A kit according to claim 16, wherein the solid phase support comprises an acrylic resin, or comprises an inorganic material treated for covalent coupling with antibody protein.
Claim 22
Independent Claim
133 words
A method for detection of proteins in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiprotein antibody (Ab) which is capable of forming immuno-complexes with antigens characteristic of the proteins to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, smaller than 0.1 μm, said microspheres being labelled with metal elements and having conjugated thereto said Ab which enables the binding of said microspheres to said immuno-complexes; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, thereby detecting or determining the presence of proteins in said specimen.
Claim 23
Independent Claim
133 words
A method for detection of proteins in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiprotein antibody (Ab) which is capable of forming immuno-complexes with antigens characteristic of the proteins to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, smaller than 0.1 μm, said microspheres being labelled with metal elements and having conjugated thereto said Ab which enables the binding of said microspheres to said immuno-complexes; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, thereby detecting or determining the presence of proteins in said specimen.
Claim 24
Dependent Claim
30 words
A method according to claim 20, wherein before contacting the solid phase support, the specimen is contacted with a polystyrene latex surface coated with gamma immunoglobulin to remove non-specific agglutinators.
Claim 25
Dependent Claim
11 words
A method according to claim 20, wherein the Ab is monoclonal.
Claim 26
Dependent Claim
27 words
A method according to claim 20, wherein the solid phase support is both separated from the specimen and also separated from the mobile solid phase by washing.
Claim 27
Dependent Claim
68 words
A method according to claim 20, wherein antispecies antibody (Ab s ) is covalently bound to the solid phase support and to the mobile solid phase, and Ab which forms an immuno-complex with said Ab s is coupled therewith, whereby there is conjugated to said solid phase support and to said mobile solid phase Ab which is capable of forming immuno-complexes with antigens of proteins to be detected.
Claim 28
Dependent Claim
11 words
A method according to claim 24, wherein the Ab is monoclonal.
Claim 29
Dependent Claim
22 words
A method according to claim 20, wherein the solid phase support forms at least part of a dipstick, syringe, tube or container.
Claim 30
Dependent Claim
32 words
A method according to claim 20, wherein the solid phase support forms at least part of a dipstick, said solid phase support being included at least at an end of said dipstick.
Claim 31
Dependent Claim
23 words
A method according to claim 27, wherein the Ab conjugated on the solid phase suport is concentrated at an end of the dipstick.
Claim 32
Dependent Claim
27 words
A method according to claim 26, wherein the solid phase support comprises an acrylic resin, or comprises an inorganic material treated for covalent coupling with antibody protein.
Claim 33
Dependent Claim
60 words
A method according to claim 20, wherein a plurality of different Ab proteins capable of forming complexes with corresponding antigens of different types of proteins are conjugated to the solid phase support and are conjugated to the mobile solid phase, whereby the presence of one or more of a plurality of different types of proteins in the specimen is detected.
Claim 34
Dependent Claim
183 words
A protein detection kit, which comprises as individual components: (a) a solid phase support having conjugated thereon antiprotein antibody (Ab) capable of forming immuno-complexes with antigens characteristic of the proteins to be detected, said antiprotein antibody consisting of a plurality of different Ab proteins capable of forming complexes with corresponding antigens of different types of proteins; and (b) a mobile solid phase consisting of dispersed microspheres less than 0.1 μm, said microspheres being labelled with a plurality of metal elements which can be detected by X-ray fluorescence and having said different Ab proteins conjugated thereto, said mobile solid phase being either in the form of a mixture of different types of microspheres, each type having conjugated thereto a different Ab protein, the different Ab proteins being capable of forming complexes with corresponding antigens of different proteins, or in the form of separate batches of microspheres, each batch having conjugated thereto a different Ab protein, said different Ab proteins being capable of forming complexes with corresponding antigens of different proteins wherein the components are present in amounts sufficient to perform the protein detection assay.
Claim 35
Dependent Claim
183 words
A protein detection kit, which comprises as individual components: (a) a solid phase support having conjugated thereon antiprotein antibody (Ab) capable of forming immuno-complexes with antigens characteristic of the proteins to be detected, said antiprotein antibody consisting of a plurality of different Ab proteins capable of forming complexes with corresponding antigens of different types of proteins; and (b) a mobile solid phase consisting of dispersed microspheres less than 0.1 μm, said microspheres being labelled with a plurality of metal elements which can be detected by X-ray fluorescence and having said different Ab proteins conjugated thereto, said mobile solid phase being either in the form of a mixture of different types of microspheres, each type having conjugated thereto a different Ab protein, the different Ab proteins being capable of forming complexes with corresponding antigens of different proteins, or in the form of separate batches of microspheres, each batch having conjugated thereto a different Ab protein, said different Ab proteins being capable of forming complexes with corresponding antigens of different proteins wherein the components are present in amounts sufficient to perform the protein detection assay.
Claim 36
Dependent Claim
25 words
A kit according to claim 31, further including as an individual component: (c) a latex solid phase capable of removing non-specific agglutinators from a specimen.
Claim 37
Dependent Claim
15 words
A kit according to claim 32, wherein said latex is polystyrene coated with gamma immuno-globulin.
Claim 38
Dependent Claim
74 words
A kit according to claim 31, wherein the solid phase support component (a) and the mobile solid phase component (b) each have antispecies antibody (Ab s ) covalently bound thereto, and Ab which forms an immuno-complex with said Ab s coupled therewith, whereby said solid phase support component (a) and said mobile solid phase component (b) each have conjugated thereto Ab which is capable of forming immuno-complex with antIgens of proteins to be detected.
Claim 39
Dependent Claim
24 words
A kit according to claim 31, wherein the solid phase support component (a) forms at least part of a dipstick, syring, tube or container.
Claim 40
Dependent Claim
32 words
A kit according to claim 31 wherein the solid phase support forms at least part of a dipstick, said solid phase support being included at least at an end of said dipstick.
Claim 41
Dependent Claim
23 words
A kit according to claim 36, wherein the Ab conjugated on the solid phase support is concentrated at an end of the dipstick.
Claim 42
Dependent Claim
27 words
A kit according to claim 35, wherein the solid phase support comprises an acrylic resin, or comprises an inorganic material treated for covalent coupling with antibody proteins.
Claim 43
Independent Claim
281 words
A method for detection of viruses in a specimen comprising: treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiviral antibody (Ab) which is capable fo forming immuno-complexes with antigens characteristic of the viruses to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, said microspheres being labelled with metal elements and having conjugated thereto said Ab v which enables the binding of said microspheres to said immuno-complexes, and wherein said Ab v conjugated to the solid phase suport and conjugated to the mobile solid phase consists of a plurality of different Ab v proteins which are capable of forming complexes with the corresponding antigens of different types of viruses, and the different Ab v proteins conjugated to the mobile solid phase are conjugated to microspheres which are correspondingly labelled with different metal elements, thereby forming a plurality of differently labelled types of microspheres, said differently labelled types of microspheres being bound to immuno-complexes of corresponding viral antigens on the solid phase support, said immunocomplexes having been formed respectively from the plurality of different Ab v proteins conjugated to the solid phase support and corresponding antigens of different types of viruses present in the specimen; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, with the diferently labelled types of microsphees being separately detected by X-ray fluorescence of the different metal element labels thereby simultaneously and separately determining the presence of corresponding individual types of viruses in the specimen.
Claim 44
Independent Claim
281 words
A method for detection of viruses in a specimen comprising: treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiviral antibody (Ab) which is capable fo forming immuno-complexes with antigens characteristic of the viruses to be detected; separating the solid phase support from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, said microspheres being labelled with metal elements and having conjugated thereto said Ab v which enables the binding of said microspheres to said immuno-complexes, and wherein said Ab v conjugated to the solid phase suport and conjugated to the mobile solid phase consists of a plurality of different Ab v proteins which are capable of forming complexes with the corresponding antigens of different types of viruses, and the different Ab v proteins conjugated to the mobile solid phase are conjugated to microspheres which are correspondingly labelled with different metal elements, thereby forming a plurality of differently labelled types of microspheres, said differently labelled types of microspheres being bound to immuno-complexes of corresponding viral antigens on the solid phase support, said immunocomplexes having been formed respectively from the plurality of different Ab v proteins conjugated to the solid phase support and corresponding antigens of different types of viruses present in the specimen; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence, with the diferently labelled types of microsphees being separately detected by X-ray fluorescence of the different metal element labels thereby simultaneously and separately determining the presence of corresponding individual types of viruses in the specimen.
Claim 45
Independent Claim
258 words
A method for detection of proteins in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiprotein antibody (Ab) which is capable of forming immuno-complexes with antigens characteristic of the proteins to be detected; separating the solid phase suport from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, said microspheres being labelled with metal elements and having conjugated thereto said Ab which enables the binding of said microspheres to said immunocomplexes; and wherein said Ab conjugated to the solid phase support and conjugated to the mobile solid phase consists of a plurality of different Ab proteins which are capable of forming complexes with corresponding antigens of different types of proteins, and the different Ab proteins conjugated to the mobile solid phase are conjugated to microspheres which are correspondingly labelled with different metal elements, thereby forming a plurality of differently labelled types of microspheres being bound to immuno-complexes having been formed respectively from the plurality of different Ab proteins conjugated to the solid phase support and corresponding antigens of different types of proteins present in the specimen; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence with the differently labelled types of microspheres being separately detected by X-ray fluorescence of the different metal element labels thereby simultaneously and separately determining the presence of corresponding individual types of proteins in the specimen.
Claim 46
Independent Claim
258 words
A method for detection of proteins in a specimen comprising; treating the specimen to remove undesired components; contacting the specimen with a solid phase support having conjugated thereto antiprotein antibody (Ab) which is capable of forming immuno-complexes with antigens characteristic of the proteins to be detected; separating the solid phase suport from the specimen; contacting said separated solid phase support with a mobile solid phase consisting of dispersed microspheres, said microspheres being labelled with metal elements and having conjugated thereto said Ab which enables the binding of said microspheres to said immunocomplexes; and wherein said Ab conjugated to the solid phase support and conjugated to the mobile solid phase consists of a plurality of different Ab proteins which are capable of forming complexes with corresponding antigens of different types of proteins, and the different Ab proteins conjugated to the mobile solid phase are conjugated to microspheres which are correspondingly labelled with different metal elements, thereby forming a plurality of differently labelled types of microspheres being bound to immuno-complexes having been formed respectively from the plurality of different Ab proteins conjugated to the solid phase support and corresponding antigens of different types of proteins present in the specimen; separating the unbound mobile solid phase from the solid phase support; and measuring the presence of microspheres bound to said solid phase support by X-ray fluorescence with the differently labelled types of microspheres being separately detected by X-ray fluorescence of the different metal element labels thereby simultaneously and separately determining the presence of corresponding individual types of proteins in the specimen.
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