US4297337A
Expired / Lapsed
US
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Solid-phase immunoassays using magnetic glass
Assignee: Bayer Corp
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Filing: 1979-04-13
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Granted: 1981-10-27
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Expires: 1999-04-13
Abstract
Abstract Solid-phase immunoassay procedures employing magnetic glass or crystal-containing material, or a superparamagnetic material formed of magnetic particles dispersed in a non-magnetic matrix, as particulate carrier.
Inventors
- 👤 Gerald R. Mansfield
- 👤 Karen L. Travis
- 👤 William P. Vann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G11; G11B; G11B5/00; G11B5/62; G11B5/68; G11B5/70; G11B5/706; G11B5/70626; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
85 words
In a process for carrying out a solid-phase immunoassay wherein a biological material is complexed with a water-insoluble, inert particulate carrier and, during the assay, the particulate carrier is separated from a fluid, the improvement which comprises utilizing, as said particulate carrier, water-insoluble, inert, magnetically-dilute particles of a glass and/or crystal-containing material having iron-containing, magnetic crystals incorporated therein with dimensions of less than about 1000 A which are essentially enveloped within the glass and/or crystal structure, and magnetically separating said particulate carrier from said fluid.
Claim 2
Dependent Claim
85 words
In a process for carrying out a solid-phase immunoassay wherein a biological material is complexed with a water-insoluble, inert particulate carrier and, during the assay, the particulate carrier is separated from a fluid, the improvement which comprises utilizing, as said particulate carrier, water-insoluble, inert, magnetically-dilute particles of a glass and/or crystal-containing material having iron-containing, magnetic crystals incorporated therein with dimensions of less than about 1000 A which are essentially enveloped within the glass and/or crystal structure, and magnetically separating said particulate carrier from said fluid.
Claim 3
Dependent Claim
20 words
A process according to claim 1 wherein said biological material is an antigen, a hapten, an enzyme, or an antibody.
Claim 4
Dependent Claim
17 words
A process according to claim 1 wherein said glass and/or crystal-containing material consists of a crystalline ceramic.
Claim 5
Dependent Claim
16 words
A process according to claim 1 wherein said glass and/or crystal-containing material consists of a glass.
Claim 6
Dependent Claim
17 words
A process according to claim 4 wherein said glass has a composition in the alkali metal-iron-borosilicate system.
Claim 7
Dependent Claim
17 words
A process according to claim 1 wherein said glass and/or crystal-containing material consists of a crystal-containing material.
Claim 8
Dependent Claim
25 words
A process according to claim 1 wherein said magnetic crystals are selected from the group of gamma iron oxide, magnetite, a ferrite, and mixtures thereof.
Claim 9
Dependent Claim
20 words
A process according to claim 1 wherein said magnetic crystals constitute less than 20% by volume of said particulate carrier.
Claim 10
Dependent Claim
14 words
A process according to claim 1 wherein said water-insoluble, inert, magnetically-dilute particles are porous.
Claim 11
Dependent Claim
14 words
A process according to claim 1 wherein said particles consist of a crystalline ceramic.
Claim 12
Dependent Claim
12 words
A process according to claim 9 wherein said particles consist of glass.
Claim 13
Dependent Claim
26 words
A process according to claim 11 wherein said particles are composed of a porous skeleton consisting essentially of vitreous silica with said magnetic crystals dispersed therewithin.
Claim 14
Dependent Claim
19 words
A process according to claim 12 wherein said magnetic crystals constitute less than 20% by volume of said particles.
Claim 15
Dependent Claim
14 words
A process according to claim 9 wherein said particles consist of a crystal-containing material.
Claim 16
Dependent Claim
39 words
A process according to claim 14 wherein said particles are composed of a glassy crystalline skeleton with said magnetic crystals enveloped therewithin, said glass consisting essentially of vitreous silica and said crystal phase consisting essentially of a silica-containing species.
Claim 17
Dependent Claim
19 words
A process according to claim 15 wherein said magnetic crystals constitute less than 20% by volume of said particles.
Claim 18
Dependent Claim
18 words
A process according to claim 1 wherein a surfactant is incorporated into said fluid during the separation operation.
Claim 19
Dependent Claim
54 words
A solid-phase immunoassay reagent consisting essentially of water-soluble, inert, magnetically-dilute carrier particles having biological material carried on the surface thereof, which particles consist essentially of a glass and/or crystal-containing material having iron-containing, magnetic crystals incorporated therein with dimensions of less than about 1000 A which are essentially enveloped within the glass and/or crystal structure.
Claim 20
Dependent Claim
54 words
A solid-phase immunoassay reagent consisting essentially of water-soluble, inert, magnetically-dilute carrier particles having biological material carried on the surface thereof, which particles consist essentially of a glass and/or crystal-containing material having iron-containing, magnetic crystals incorporated therein with dimensions of less than about 1000 A which are essentially enveloped within the glass and/or crystal structure.
Claim 21
Dependent Claim
20 words
A reagent according to claim 18 wherein said biological material is an antigen, a hapten, an enzyme, or an antibody.
Claim 22
Dependent Claim
17 words
A reagent according to claim 18 wherein said glass and/or crystal-containing material consists of a crystalline ceramic.
Claim 23
Dependent Claim
16 words
A reagent according to claim 18 wherein said glass and/or crystal-containing material consists of a glass.
Claim 24
Dependent Claim
17 words
A reagent according to claim 21 wherein said glass has a composition in the alkali metal-iron-borosilicate system.
Claim 25
Dependent Claim
17 words
A reagent according to claim 18 wherein said glass and/or crystal-containing material consists of a crystal-containing material.
Claim 26
Dependent Claim
25 words
A reagent according to claim 18 wherein said magnetic crystals are selected from the group of gamma iron oxide, magnetite, a ferrite, and mixtures thereof.
Claim 27
Dependent Claim
20 words
A reagent according to claim 18 wherein said magnetic crystals constitute less than 20% by volume of said carrier particles.
Claim 28
Dependent Claim
11 words
A reagent according to claim 18 wherein said particles are porous.
Claim 29
Dependent Claim
14 words
A reagent according to claim 26 wherein said particles consist of a crystalline ceramic.
Claim 30
Dependent Claim
12 words
A reagent according to claim 26 wherein said particles consist of glass.
Claim 31
Dependent Claim
26 words
A reagent according to claim 28 wherein said particles are composed of a porous skeleton consisting essentially of vitreous silica with said magnetic crystals dispersed therewithin.
Claim 32
Dependent Claim
19 words
A reagent according to claim 29 wherein said magnetic crystals constitute less than 20% by volume of said particles.
Claim 33
Dependent Claim
14 words
A reagent according to claim 26 wherein said particles consist of a crystal-containing material.
Claim 34
Dependent Claim
38 words
A reagent according to claim 31 wherein said particles are composed of a glassy-crystalline skeleton with said magnetic crystals enveloped therewithin, said glass consisting essentially of vitreous silica and said crystal phase consisting essentially of a silica-containing phase.
Claim 35
Dependent Claim
19 words
A reagent according to claim 32 wherein said magnetic crystals constitute less than 20% by volume of said particles.
Claim 36
Dependent Claim
9 words
A radioimmunoassay kit containing the reagent of claim 18.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-26 | 1985-04-27 | 1985-10-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-26 | 1989-04-27 | 1989-10-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-26 | 1993-04-27 | 1993-10-27 | $7700.00 | Window Closed / Lapsed |