Abstract This invention relates to a process for producing immobilized L-asparaginase preparations. Its principal object is to produce immobilized L-asparaginase preparations which are excellent in antithrombogenicity and mechanical strength. The present invention is concerned with production of immobilized L-asparaginase preparations by pouring an aqueous solution containing 6% or more of a polyvinyl alcohol with a degree of hydrolysis of 97 mol. % or higher and a viscosity-average degree of polymerization of 1,800 or more an antileukemic asparaginase into a vessel or a mold of an appropriate shape, subjecting the solution to cooling, solidification and molding at a temperature of -15° C. or lower and partially dehydrating the molded mass without thawing to a dehydration ratio of 5% by weight or more and, if desired, immersing the product in water. According to the invention, L-asparaginase can be embedded in a highly hydrous gel excellent in antithrombogenicity and mechanical strength by simple procedures.
A process for producing immobilized L-asparaginase preparations for the therapy of leukemia which comprises pouring an aqueous solution containing 6% by weight or more of a polyvinyl alcohol with a degree of hydrolysis of 97 mol. % or higher and a degree of polymerization of 1,800 or more and an antileukemic asparaginase into a vessel of a desired shape and subjecting the solution to cooling and solidification at a temperature lower then -15° C., and then partially dehydrating the resulting solidified mass without thawing to a dehydration ratio of 5% by weight or more to obtain a desired gel.
Claim 2
Dependent Claim
99 words
A process for producing immobilized L-asparaginase preparations for the therapy of leukemia which comprises pouring an aqueous solution containing 6% by weight or more of a polyvinyl alcohol with a degree of hydrolysis of 97 mol. % or higher and a degree of polymerization of 1,800 or more and an antileukemic asparaginase into a vessel of a desired shape and subjecting the solution to cooling and solidification at a temperature lower then -15° C., and then partially dehydrating the resulting solidified mass without thawing to a dehydration ratio of 5% by weight or more to obtain a desired gel.
Claim 3
Dependent Claim
14 words
The process according to claim 1 wherein heparin is added to the aqueous solution.
Claim 4
Dependent Claim
25 words
The process according to claim 1 or 2 wherein concentration of the polyvinyl alcohol in the aqueous solution is from 6 to 25% by weight.
Claim 5
Dependent Claim
21 words
The process according to any one of claims 1-3 wherein the cooling, solidification and molding temperature is -35° C. or below.
Claim 6
Dependent Claim
23 words
The process according to any one of claim 1-4 wherein dehydration ratio in the partial dehydration step is 15% by weight or more.
Claim 7
Dependent Claim
18 words
The process according to any one of claims 1-5 wherein the partial dehydration is conducted by vacuum dehydration.
Claim 8
Dependent Claim
37 words
The process according to any one of the claims 1-6 wherein the water content of the obtained gel is increased by immersing said gel in water to give a water content of 45 to 92% by weight.