US4597999A
Expired / Lapsed
US
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Method for coupling a hydrocarbon containing molecular species
Assignee: Hospital for Sick Children
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Filing: 1984-10-04
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Granted: 1986-07-01
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Expires: 2004-10-04
Abstract
Abstract The present invention relates to a method for covalently coupling two molecular species and to the product of such coupling. The method has particular application to the binding of hydrocarbon containing ligands to a support matrix as may be required in, for example, affinity chromatography.
Inventors
- 👤 Clifford A. Lingwood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:33
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/52; B01J20/3206; B01J20/3208; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3268; B01J20/328; B01J20/3282; B01J20/3285; B01J20/3291; B01J20/3293; C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
63 words
A method of covalently coupling two molecular species comprising: (a) combining (i) a first molecular species having a functionality reactive with hydrocarbon when photo-activated; and (ii) a solution of at least one, hydrocarbon containing, molecular species in the absence of photo-radiation to which said functionality is sensitive; (b) removing the solvent; (c) irradiating the mixture with photo-radiation to which said functionality is photosensitive.
Claim 2
Independent Claim
63 words
A method of covalently coupling two molecular species comprising: (a) combining (i) a first molecular species having a functionality reactive with hydrocarbon when photo-activated; and (ii) a solution of at least one, hydrocarbon containing, molecular species in the absence of photo-radiation to which said functionality is sensitive; (b) removing the solvent; (c) irradiating the mixture with photo-radiation to which said functionality is photosensitive.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein the first molecular species is a support matrix.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein the support matrix is comprised of beads.
Claim 5
Dependent Claim
20 words
The method of claim 1, 2 or 3 wherein the functionality reactive with hydrocarbon when photo-activated is an azide group.
Claim 6
Independent Claim
94 words
A method of covalently coupling two molecular species comprising: (a) combining (i) a first molecular species; and (ii) a heterobifunctional cross-linking agent having a first functionality reactive with said first molecular species and a second functionality reactive with hydrocarbon when photo-activated; in the absence of photo-radiation to which said second functionality is photosensitive; (b) adding a solution containing at least one, hydrocarbon containing, molecular species, in the absence of photo-radiation to which said second functionality is sensitive; (c) removing the solvent; (d) irradiating the mixture with photo-radiation to which said second functionality is photosensitive.
Claim 7
Independent Claim
94 words
A method of covalently coupling two molecular species comprising: (a) combining (i) a first molecular species; and (ii) a heterobifunctional cross-linking agent having a first functionality reactive with said first molecular species and a second functionality reactive with hydrocarbon when photo-activated; in the absence of photo-radiation to which said second functionality is photosensitive; (b) adding a solution containing at least one, hydrocarbon containing, molecular species, in the absence of photo-radiation to which said second functionality is sensitive; (c) removing the solvent; (d) irradiating the mixture with photo-radiation to which said second functionality is photosensitive.
Claim 8
Dependent Claim
14 words
The method of claim 5 wherein the first molecular species is a support matrix.
Claim 9
Dependent Claim
13 words
The method of claim 6 wherein the support matrix is comprised of beads.
Claim 10
Dependent Claim
18 words
The method of claim 6 wherein the second functionality reactive with hydrocarbon when photo-activated is an azide group.
Claim 11
Dependent Claim
39 words
The method of claim 8 wherein the support matrix contains at least one functional group selected from the group consisting of amino and thiol and the first functionality on said heterobifunctional cross-linking agent is reactive with said functional group.
Claim 12
Dependent Claim
15 words
The method of claim 8 wherein the heterobifunctional cross-linking agent further contains a cleavable functionality.
Claim 13
Dependent Claim
19 words
The method of claim 10 wherein the cleavable functionality is selected from the group consisting of azo and disulfide.
Claim 14
Dependent Claim
30 words
The method of claim 6 wherein the support matrix contains at least one amino group and the heterobifunctional cross-linking agent is selected from the group consisting of 4-methylazidobenzimidate and N-hydroxysuccinimidylazidobenzoate.
Claim 15
Dependent Claim
42 words
The method of claim 5 or 6 wherein the first molecular species contains attached thereto a functional group selected from the group consisting of amino and thiol and the first functionality on said heterobifunctional cross-linking agent is reative with said functional group.
Claim 16
Dependent Claim
17 words
The method of claim 5 or 6 wherein the heterobifunctional cross-linking agent further contains a cleavable functionality.
Claim 17
Dependent Claim
26 words
The method of claim 5 or 6 wherein the heterobifunctional cross-linking agent further contains a cleavable functionality selected from the group consisting of azo and disulfide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-30 | 1989-12-30 | 1990-07-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-30 | 1993-12-30 | 1994-07-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-30 | 1997-12-30 | 1998-07-01 | $7700.00 | Window Closed / Lapsed |