US4564596A
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US
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Urokinase derivatives covalently bound to fibrinogen
Assignee: VSESOJUZNY KARDIOLOGICHESKY NAUCHNY TSENTR AKADEMII MEDITSINSKIKH NAUK SSSR USSR MOSCOW 3 CHEREPKOVSKAYA ULITSA 15A
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Filing: 1984-03-01
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Granted: 1986-01-14
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Expires: 2004-03-01
Abstract
Abstract Urokinase derivatives comprise urokinase covalently bonded with fibrinogen or urokinase covalently bonded with fibrinogen through an aliphatic diamine and correspond to the general formula: ##STR1## wherein P is fibrinogen, E is urokinase, R is absent or stands for ##STR2## wherein n is 1-12, with a molecular mass of from 360,000 to 440,000 D, a content of protein of 10 to 30% by mass, an esterase catalytic activity of 30-60%. These derivatives have an increased affinity to fibrin and feature a prolonged fibrinolytic effect.
Inventors
- 👤 Alexandr V. Maximenko
- 👤 Elena G. Tischenko
- 👤 Vladimir P. Torchilin
- 👤 Vladimir N. Smirnov
- 👤 Evgeny I. Chazov
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/62; A61P; A61P7/00; A61P7/02; C12N9/96; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; A61K38/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
67 words
Urokinase derivatives comprising urokinase covalently bonded with fibrinogen or urokinase covalently bonded, through an aliphatic diamine, with fibrinogen of the general formula: ##STR6## wherein P is fibrinogen, E is urokinase, R is either a single bond or stands for ##STR7## wherein n is 1-12, with a molecular mass of 360,000 to 440,000D, a content of protein of 10-30% by mass, and an esterase catalytic activity of 30-60%.
Claim 2
Dependent Claim
67 words
Urokinase derivatives comprising urokinase covalently bonded with fibrinogen or urokinase covalently bonded, through an aliphatic diamine, with fibrinogen of the general formula: ##STR6## wherein P is fibrinogen, E is urokinase, R is either a single bond or stands for ##STR7## wherein n is 1-12, with a molecular mass of 360,000 to 440,000D, a content of protein of 10-30% by mass, and an esterase catalytic activity of 30-60%.
Claim 3
Dependent Claim
12 words
The urokinase derivative of claim 1 wherein said aliphatic diamine is 1,12-dodecamethylenediamine.
Claim 4
Dependent Claim
12 words
The urokinase derivative of claim 1 wherein said aliphatic diamine is 1,10-decamethylenediamine.
Claim 5
Dependent Claim
12 words
The urokinase derivative of claim 1 wherein said aliphatic diamine is 1,7-heptamethylenediamine.
Claim 6
Dependent Claim
12 words
The urokinase derivative of claim 1 wherein said aliphatic diamine is 1,4-tetramethylenediamine.
Claim 7
Dependent Claim
12 words
The urokinase derivative of claim 1 wherein R represents a single bond.
Claim 8
Dependent Claim
85 words
A process for the preparation of a urokinase derivative of the formula: ##STR8## which comprises reacting a compound of the formula: ##STR9## with a compound of the formula: ##STR10## wherein P is fibrinogen, E is urokinase, R represents a single bond or ##STR11## R 1 is C 2 H 5 , R 2 is ##STR12## and n is 1-12, and recovering said derivative having a molecular mass of 360,000 to 440,000D, a protein content of 10-30% by mass, and an esterase catalytic activity of 30-60%.
Claim 9
Dependent Claim
85 words
A process for the preparation of a urokinase derivative of the formula: ##STR8## which comprises reacting a compound of the formula: ##STR9## with a compound of the formula: ##STR10## wherein P is fibrinogen, E is urokinase, R represents a single bond or ##STR11## R 1 is C 2 H 5 , R 2 is ##STR12## and n is 1-12, and recovering said derivative having a molecular mass of 360,000 to 440,000D, a protein content of 10-30% by mass, and an esterase catalytic activity of 30-60%.
References Cited (Backward Citations)
US3544427A
Cited
US4029767A
Cited
US4011142A
Cited
JPS54113488A
Cited
US4244943A
Cited
US4286063A
Cited
US4349630A
Cited
SU1022988A1
Cited
US4381346A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-13 | 1989-07-15 | 1990-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-13 | 1993-07-15 | 1994-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-13 | 1997-07-15 | 1998-01-14 | $7700.00 | Window Closed / Lapsed |