US4244943A
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Method for preparing urokinase injection
Assignee: Sumitomo Chemical Co Ltd
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Filing: 1979-05-09
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Granted: 1981-01-13
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Expires: 1999-05-09
Abstract
Abstract A method for preparing a stable urokinase injection by the liophilization of urokinase which comprises liophilizing an aqueous solution containing urokinase, human serum albumin and one or more amino acid compounds selected from polar amino acids and salt thereof. The urokinase injection obtained according to this method is excellent in the storing stability as compared with conventional urokinase preparations for injection.
Inventors
- 👤 Yoshiya Yamahira
- 👤 Keiji Fujioka
- 👤 Yoshiko Okuzawa
- 👤 Seiko Miura
- 👤 Shigeji Sato
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:42
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; A; A61; A61K; A61K47/00; A61K47/06; A61K47/16; A61K47/18; A61K47/183; A61K47/30; A61K47/42; A61K9/00; A61K9/0012; A61K9/0019; C12N9/96; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
72 words
A method for preparing a stable urokinase composition suitable for injection into the human body by the lyophilization of urokinase which comprises lyophilizing an aqueous solution containing urokinase, human serum albumin and one or more amino acid compounds selected from the group consisting of glutamic acid, threonine, histidine, serine, glutamine, aspartic acid, arginine and salts thereof, said amino acid compound and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 2
Independent Claim
72 words
A method for preparing a stable urokinase composition suitable for injection into the human body by the lyophilization of urokinase which comprises lyophilizing an aqueous solution containing urokinase, human serum albumin and one or more amino acid compounds selected from the group consisting of glutamic acid, threonine, histidine, serine, glutamine, aspartic acid, arginine and salts thereof, said amino acid compound and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 3
Dependent Claim
22 words
A method according to claim 1, wherein the amino acid compound is glutamic acid or sodium salt thereof, threonine, arginine or histidine.
Claim 4
Independent Claim
49 words
A method for preparing a stable urokinase composition suitable for injection into the human body by the lyophilization of urokinase which comprises lyophilizing an aqueous solution containing urokinase, human serum albumin and glutamic acid, said glutamic acid and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 5
Independent Claim
49 words
A method for preparing a stable urokinase composition suitable for injection into the human body by the lyophilization of urokinase which comprises lyophilizing an aqueous solution containing urokinase, human serum albumin and glutamic acid, said glutamic acid and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 6
Dependent Claim
43 words
A method according to claim 1, wherein the ratio of a mixture of human serum albumin and the amino acid compound to urokinase is 10-50 mg of the sum of human serum albumin and the amino acid compound to 6,000-60,000 IU of urokinase.
Claim 7
Dependent Claim
23 words
A method according to claim 4, wherein the weight ratio of human serum albumin to the amino acid compound is 1:0.05 to 1:10.
Claim 8
Dependent Claim
14 words
A method according to claim 5, wherein the weight ratio is 1:0.1 to 1:4.
Claim 9
Dependent Claim
50 words
A lyophilized urokinase composition comprising urokinase, human serum albumin, and one or more amino acid compounds selected from the group consisting of glutamic acid, threonine, histidine, serine, glutamine, aspartic acid, arginine and salts thereof, said amino acid compound and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 10
Dependent Claim
50 words
A lyophilized urokinase composition comprising urokinase, human serum albumin, and one or more amino acid compounds selected from the group consisting of glutamic acid, threonine, histidine, serine, glutamine, aspartic acid, arginine and salts thereof, said amino acid compound and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 11
Dependent Claim
34 words
A lyophilized urokinase composition according to claim 7, wherein the amino acid compound is glutamic acid or sodium salt thereof, threonine, arginine hydrochloric acid salt of arginine, histidine or hydrochloric acid salt of histidine.
Claim 12
Dependent Claim
27 words
A lyophilized urokinase composition comprising urokinase, human serum albumin and glutamic acid, said glutamic acid and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 13
Dependent Claim
27 words
A lyophilized urokinase composition comprising urokinase, human serum albumin and glutamic acid, said glutamic acid and human serum albumin being present in amounts effective to stabilize urokinase.
Claim 14
Dependent Claim
43 words
A lyophilized urokinase composition according to claim 7, wherein the ratio of human serum albumin and the amino acid compound to urokinase is 10-50 mg of the sum of human serum albumin and the amino acid compound to b 6,000-60,000 IU of urokinase.
Claim 15
Dependent Claim
25 words
A lyophilized urokinase composition according to claim 7, wherein the weight ratio of human serum albumin to the amino acid compound is 1:0.05 to 1:10.
Claim 16
Dependent Claim
16 words
A lyophilized urokinase composition according to claim 11, wherein the weight ratio is 1:0.1 to 1:4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-01-13 | 1984-07-14 | 1985-01-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-01-13 | 1988-07-14 | 1989-01-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-01-13 | 1992-07-14 | 1993-01-13 | $7700.00 | Window Closed / Lapsed |