Novel antitumor glycoprotein substance and its preparation

Claim 2

A glycoprotein substance possessing an antitumor activity which is recovered from the liquid portion coming from cooking of raw scallop shellfish with a hot aqueous solvent or with the vapor of such solvent and which has the following characteristics: (1) Appearance: White, powdery solid; (2) Solubility: Soluble in water, but insoluble in organic solvents such as methanol, ethanol and acetone; (3) Acidic or basic nature: Amphoteric electrolyte having an isoelectric point of pH 5.2; (4) Infrared absorption spectrum (pelleted in KBr): With characteristic absorption peaks at 3500˜3300, 1660 and 1550 cm -1 ; (5) Ultraviolet absorption spectrum in an aqueous solution: With a characteristic absorption peak λ max H .sbsp.2 O at 279 nm; (6) Color reactions: Positive in biuret reaction, xanthoproteic reaction, phenolic reagent reaction according to Lowry-Folin method, anthrone-sulfuric acid reaction and phenol-sulfuric acid reaction, but negative in cysteine-sulfuric acid reaction; (7) Average molecular weight: About 5000 as measured by gel-electrophoresis.

Claim 3

A glycoprotein substance possessing an antitumor activity which is recovered from the liquid portion coming from cooking of raw scallop shellfish with a hot aqueous solvent or with the vapor of such solvent by concentrating the liquid portion to a smaller volume or to dryness to give a concentrate or dry powder, dissolving the concentrate or dry powder in water to form an aqueous solution thereof, subjecting the aqueous solution to a series of treatments comprising ion-exchange chromatography with a basic anion exchanger, isoelectric focusing and molecular weight-fractionating treatments comprising gel-filtration and electrophoresis, in any desired sequence, and which has the following characteristics: (1) Appearance: White, powdery solid; (2) Solubility: Soluble in water, but insoluble in organic solvents such as methanol, ethanol and acetone; (3) Acidic or basic nature: Amphoteric electrolyte having an isoelectric point of pH 5.2; (4) Infrared absorption spectrum (pelleted in KBr): With characteristic absorption peaks at 3500˜3300, 1660 and 1550 cm -1 ; (5) Ultraviolet absorption spectrum in an aqueous solution: With a characteristic absorption peak λ max H .sbsp.2 O at 279 nm; (6) Color reactions: Positive in biuret reaction, xanthoproteic reaction, phenolic reagent reaction according to Lowry-Folin method, anthrone-sulfuric acid reaction and phenol-sulfuric acid reaction, but negative in cysteine-sulfuric acid reaction; (7) Average molecular weight: About 5000 as measured by gel-electrophoresis.

Claim 4

A glycoprotein substance possessing an antitumor activity which is recovered from the liquid portion coming from cooking of raw scallop shellfish with a hot aqueous solvent or with the vapor of such solvent by concentrating the liquid portion to a smaller volume or to dryness to give a concentrate or dry powder, dissolving the concentrate or dry powder in water to form an aqueous solution thereof, subjecting the aqueous solution to a series of treatments comprising ion-exchange chromatography with a basic anion exchanger, isoelectric focusing and molecular weight-fractionating treatments comprising gel-filtration and electrophoresis, in any desired sequence, and which has the following characteristics: (1) Appearance: White, powdery solid; (2) Solubility: Soluble in water, but insoluble in organic solvents such as methanol, ethanol and acetone; (3) Acidic or basic nature: Amphoteric electrolyte having an isoelectric point of pH 5.2; (4) Infrared absorption spectrum (pelleted in KBr): With characteristic absorption peaks at 3500˜3300, 1660 and 1550 cm -1 ; (5) Ultraviolet absorption spectrum in an aqueous solution: With a characteristic absorption peak λ max H .sbsp.2 O at 279 nm; (6) Color reactions: Positive in biuret reaction, xanthoproteic reaction, phenolic reagent reaction according to Lowry-Folin method, anthrone-sulfuric acid reaction and phenol-sulfuric acid reaction, but negative in cysteine-sulfuric acid reaction; (7) Average molecular weight: About 5000 as measured by gel-electrophoresis.

Claim 5

A process for the preparation of a glycoprotein substance having an average molecular weight of about 5000 and an isoelectric point of pH 5.2 from scallop shellfish which comprises recovering the liquid portion which comes from cooking of raw scallop shellfish with a hot aqueous solvent or with vapor of such solvent and which is to be discarded as waste, concentrating the liquid portion to a smaller volume or to dryness to yield a concentrate or dry powder, dissolving the concentrate or dry powder in water to form an aqueous solution thereof, subjecting the aqueous solution to a series of treatments comprising ion-exchange chromatography with a basic anion exchanger, isoelectric focusing and molecular weight-fractionating treatments comprising gel-filtration and electrophoresis, in any desired sequence, whereby to isolate a glycoprotein substance which is adsorbable on the basic ion exchanger and which has an average molecular weight of about 5000 and an isoelectric point of pH 5.2.

Claim 6

A process for the preparation of a glycoprotein substance having an average molecular weight of about 5000 and an isoelectric point of pH 5.2 from scallop shellfish which comprises recovering the liquid portion which comes from cooking of raw scallop shellfish with a hot aqueous solvent or with vapor of such solvent and which is to be discarded as waste, concentrating the liquid portion to a smaller volume or to dryness to yield a concentrate or dry powder, dissolving the concentrate or dry powder in water to form an aqueous solution thereof, subjecting the aqueous solution to a series of treatments comprising ion-exchange chromatography with a basic anion exchanger, isoelectric focusing and molecular weight-fractionating treatments comprising gel-filtration and electrophoresis, in any desired sequence, whereby to isolate a glycoprotein substance which is adsorbable on the basic ion exchanger and which has an average molecular weight of about 5000 and an isoelectric point of pH 5.2.

Claim 7

A process according to claim 3 wherein after the concentrate or dry powder is dissolved in water, the aqueous solution so formed is subjected to ion-exchange chromatography with a basic anion exchanger to isolate a fraction adsorbed on the basic anion exchanger and the fraction is then subjected to isoelectric focusing to isolate a fraction containing substances having an isoelectric point of pH 5.2 and the fraction thus isolated is subjected to molecular weight-fractionating treatments comprising gel-filtration and electrophoresis to isolate a substance having an average molecular weight of about 5000.