Lipid membrane electroanalytical elements and method of analysis therewith

Claim 3

A device according to claim 1, wherein said electrically conductive layer is a doped silicon layer, said first surface is silicon oxide, and said molecules of said second layer are individual molecules.

Claim 4

A device according to claim 1, wherein said electrically conductive layer is a doped silicon layer, said first surface is silicon oxide, and said molecules of said second layer are at least in part cross-linked.

Claim 5

A device according to claim 1, wherein said electrically conductive layer is a doped silicon layer defining an FET device having a gate, with said first surface being silicon dioxide over said gate.

Claim 6

A device according to claim 1, wherein said electrically conductive layer is a metal.

Claim 7

A device according to claim 1, wherein a specific binding member is bound to said second hydrophobic layer.

Claim 8

A device according to claim 6, wherein an enzyme is bound to said second hydrophobic layer.

Claim 9

A method for detecting a change in the polarity of a polar medium which comprises contacting a device according to claim 1 with said polar medium and a reference electrode; and detecting the change in potential between said electrode and said device by effecting a change in the polar state of said medium adjacent to said device.

Claim 10

A method according to claim 8, wherein a member of a specific binding pair is bound to said second hydrophobic layer and said change is a result of binding of specific binding pair members.

Claim 11

A semiconductive device comprising: a solid semiconductive layer having at least two areas of different dopants; means for detecting a change in the electrical properties of said semiconductive layer; an insulating oxide or nitride layer on a surface of said conductive layer; bound to said insulating layer, a first hydrophobic layer comprising a plurality of molecules having aliphatic chains of at least six carbon atoms, non-diffusively bound to said insulating layer; a second stable lamellar hydrophobic lipid layer comprising molecules having aliphatic chains of at least six carbon atoms and polar heads with said polar heads defining an electrostatically charged layer distal from said first hydrophobic layer, and wherein said individual molecules are laterally diffusible; wherein said first and second hydrophobic layers define a bilayer membrane.

Claim 12

A semiconductive device comprising: a solid semiconductive layer having at least two areas of different dopants; means for detecting a change in the electrical properties of said semiconductive layer; an insulating oxide or nitride layer on a surface of said conductive layer; bound to said insulating layer, a first hydrophobic layer comprising a plurality of molecules having aliphatic chains of at least six carbon atoms, non-diffusively bound to said insulating layer; a second stable lamellar hydrophobic lipid layer comprising molecules having aliphatic chains of at least six carbon atoms and polar heads with said polar heads defining an electrostatically charged layer distal from said first hydrophobic layer, and wherein said individual molecules are laterally diffusible; wherein said first and second hydrophobic layers define a bilayer membrane.

Claim 13

A device according to claim 10, wherein said semiconductive layer is silicon and said insulative layer is of from about 10 to 500Å thick.

Claim 14

A device according to claim 11, wherein said insulative layer is of from about 10 to 100Å thick and said molecules of said second hydrophobic layer have aliphatic chains of from about 14 to 24 carbon atoms and are individual molecules.

Claim 15

A device according to claim 11, wherein said insulative layer is of from about 10 to 100Å thick and said molecules of said second hydrophobic layer have aliphatic chains of from about 14 to 24 carbon atoms and are at least in part cross-linked molecules.

Claim 16

A device according to claim 10, wherein a member of a specific binding pair is bound to said second hydrophobic layer.

Claim 17

A device according to claim 14, wherein said member of said specific binding pair is a ligand.

Claim 18

A device according to claim 14, wherein said member of said specific binding pair is a receptor.

Claim 19

A device according to claim 16, wherein said receptor is an enzyme.

Claim 20

A device according to claim 10, wherein said first hydrophobic layer is formed by reacting alkylsilyl halides with said insulating layer.

Claim 21

A method for detecting a species in an aqueous medium, wherein said species is a member of a specific binding pair, said method comprising: immersing in said aqueous medium a first electrode comprising a device according to claim 10 and a reference electrode, wherein a first member of said specific binding pair is bound to said second hydrophobic layer and said aqueous medium is suspected of containing a second member which is the reciprocal binding member of said first member; and detecting the change of potential between said electrodes as related to the amount of said second member which binds to said first member.

Claim 22

A field effect transistor transducer for detection of a change in the electrostatic environment in an aqueous medium comprising: a semiconductor substrate having a gate and source and drain regions spaced apart by said gate; means for detecting an electrical signal from said source and drain regions; an insulating layer above said gate; a first hydrophobic layer comprising a plurality of molecules having aliphatic chains of at least six carbon atoms, non-diffusively bound to said insulating layer; a second stable lamellar hydrophobic lipid layer comprising individual molecules having chains of at least six carbon atoms and polar heads, wherein said polar heads define a polar layer distal from said first layer, and wherein said individual molecules are laterally diffusible and non-covalently bound to said first layer; wherein said first and second hydrophobic layers define a bilayer membrane.

Claim 23

A field effect transistor transducer for detection of a change in the electrostatic environment in an aqueous medium comprising: a semiconductor substrate having a gate and source and drain regions spaced apart by said gate; means for detecting an electrical signal from said source and drain regions; an insulating layer above said gate; a first hydrophobic layer comprising a plurality of molecules having aliphatic chains of at least six carbon atoms, non-diffusively bound to said insulating layer; a second stable lamellar hydrophobic lipid layer comprising individual molecules having chains of at least six carbon atoms and polar heads, wherein said polar heads define a polar layer distal from said first layer, and wherein said individual molecules are laterally diffusible and non-covalently bound to said first layer; wherein said first and second hydrophobic layers define a bilayer membrane.

Claim 24

A transducer according to claim 20, wherein said semiconductor substrate is silicon.

Claim 25

A transducer according to claim 21, wherein said molecules of said first and second layers have aliphatic chains of from 6 to 24 carbon atoms.

Claim 26

A transducer according to claim 22, wherein a member of a specific binding pair is bound to said second hydrophobic layer.