US4467031A
Expired / Lapsed
US
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Enzyme-immunoassay for carcinoembryonic antigen
Assignee: F Hoffmann La Roche AG
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Filing: 1982-08-16
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Granted: 1984-08-21
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Expires: 2002-08-16
Abstract
Abstract A solid phase-sandwich enzyme-immunoassay method for the rapid determination of carcinoembryonic antigen (CEA). The sample to be investigated is incubated with a first CEA antibody, which is bound to a water-insoluble carrier, and a second CEA antibody, to which peroxidase is bound directly or via a biotin/avidin bridge. The immunological reaction can be carried out in one or two steps. By the presence of 0.4-1.0 mol/l of phosphate ions, 0.3-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions in the immunological reaction or in the second step thereof (insofar as it is carried out in two steps) the incubation time period is shortened considerably. After the immunological reaction, the phases are separated, whereupon the peroxidase activity is measured either in the solid or in the liquid phase as the amount of CEA present in the sample.
Inventors
- 👤 Harald Gallati
- 👤 Hans Brodbeck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57565; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/962; Y10S435/963; Y10S435/975; Y10S436/00; Y10S436/811; Y10S436/813; Y10S436/826
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
110 words
In a solid phase-sandwich method for the rapid determination of carcino-embryonic antigen (CEA) which comprises incubating a sample to be investigated with a first CEA antibody, which is bound to or becomes bound to a water-insoluble carrier, and a second CEA antibody, to which peroxidase is bound directly or via a biotin/avidin bridge, separating the solid and liquid phase and measuring of the peroxidase activity either in the solid or in the liquid phase as the amount of CEA present, wherein the improvement comprises carrying out the incubation in the presence of 0.4-1.0 mol/l of phosphate ions, of 0.2-0.4 mol/l of sulfate ions or of 0.2-0.4 mol/l of tartrate ions.
Claim 2
Dependent Claim
110 words
In a solid phase-sandwich method for the rapid determination of carcino-embryonic antigen (CEA) which comprises incubating a sample to be investigated with a first CEA antibody, which is bound to or becomes bound to a water-insoluble carrier, and a second CEA antibody, to which peroxidase is bound directly or via a biotin/avidin bridge, separating the solid and liquid phase and measuring of the peroxidase activity either in the solid or in the liquid phase as the amount of CEA present, wherein the improvement comprises carrying out the incubation in the presence of 0.4-1.0 mol/l of phosphate ions, of 0.2-0.4 mol/l of sulfate ions or of 0.2-0.4 mol/l of tartrate ions.
Claim 3
Dependent Claim
79 words
The method according to claim 1, wherein the sample is incubated with the first CEA antibody, which is bound to or becomes bound to a water-insoluble carrier, and, after a washing step, is incubated with the second CEA antibody, to which peroxidase is bound directly or via a biotin/avidin bridge, whereby the second incubation is carried out in the presence of 0.4-1.0 mol/l of phosphate ions, of 0.2-0.4 mol/l of sulfate ions or of 0.2-0.4 mol/l of tartrate ions.
Claim 4
Dependent Claim
20 words
The method according to claim 1 or claim 2, wherein the peroxidase is bound directly to the second CEA antibody.
Claim 5
Dependent Claim
30 words
The method according to claim 3, wherein the two CEA antibodies are obtained from antiserum of different animal species and wherein peroxidase is bound directly to one of the antibodies.
Claim 6
Dependent Claim
28 words
The method according to claim 4, wherein CEA antibodies from goat and guinea pig are used and wherein in the peroxidase is bound directly to the goat antibody.
Claim 7
Dependent Claim
33 words
The method according to claim 1 or claim 2, wherein two different monoclonal mouse-CEA antibodies are used and wherein the peroxidase is bound to one of the monoclonal antibodies via a biotin/avidin bridge.
Claim 8
Dependent Claim
43 words
The method according to claim 3, wherein there is used a CEA antibody, which is obtained from an antiserum of an animal, as well as a monoclonal mouse-CEA antibody and wherein the peroxidase is bound directly to the antibody obtained from an antiserum.
Claim 9
Dependent Claim
31 words
The method according to claim 7, wherein as the CEA antibodies there are used goat-CEA antibody and monoclonal mouse-CEA antibody and wherein the peroxidase is bound directly to the goat-CEA antibody.
Claim 10
Dependent Claim
40 words
CEA antibody, to which peroxidase is coupled directly or via a biotin/avidin bridge, in an aqueous solution of pH 4-9, wherein the solution contains 0.4-1.0 mol/l of phosphate ions, 0.2-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions.
Claim 11
Dependent Claim
40 words
CEA antibody, to which peroxidase is coupled directly or via a biotin/avidin bridge, in an aqueous solution of pH 4-9, wherein the solution contains 0.4-1.0 mol/l of phosphate ions, 0.2-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions.
Claim 12
Dependent Claim
15 words
CEA antibody according to claim 9, wherein the peroxidase is coupled directly to the antibody.
Claim 13
Dependent Claim
66 words
A test kit for determining carcinoembryonic antigen (CEA) in a sample, comprising: (a) a first CEA antibody which is bound to water-insoluble carrier; (b) a second CEA antibody labeled with peroxidase wherein the peroxidase is bound directly or via a biotin/avidin bridge; (c) an aqueous solution of pH 4-9 comprising 0.4-1.0 mol/l of phosphate ions, 0.2-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions.
Claim 14
Dependent Claim
66 words
A test kit for determining carcinoembryonic antigen (CEA) in a sample, comprising: (a) a first CEA antibody which is bound to water-insoluble carrier; (b) a second CEA antibody labeled with peroxidase wherein the peroxidase is bound directly or via a biotin/avidin bridge; (c) an aqueous solution of pH 4-9 comprising 0.4-1.0 mol/l of phosphate ions, 0.2-0.4 mol/l of sulfate ions or 0.2-0.4 mol/l of tartrate ions.
Claim 15
Dependent Claim
32 words
The test kit according to claim 11, wherein the two CEA antibodies are obtained from antiserum of different animal species and wherein the peroxidase is bound directly to one of the antibodies.
Claim 16
Dependent Claim
28 words
A test kit according to claim 11, wherein CEA antibodies from goat and guinea pig are used and wherein the peroxidase is bound directly to the goat antibody.
Claim 17
Dependent Claim
31 words
The test kit according to claim 11, wherein two different monoclonal mouse-CEA antibodies are used and wherein the peroxidase is bound to one of the monoclonal antibodies via a biotin/avidin bridge.
Claim 18
Dependent Claim
44 words
The test kit according to claim 11, wherein there is used a CEA antibody, which is obtained from an antiserum of an animal, as well as a monoclonal mouse-CEA antibody and wherein the peroxidase is bound directly to the antibody obtained from an antiserum.
Claim 19
Dependent Claim
32 words
The test kit according to claim 11, wherein as the CEA antibodies there are used goat-CEA antibody and monoclonal mouse-CEA antibody and wherein the peroxidase is bound directly to the goat-CEA antibody.
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WO1981001469A1
Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-21 | 1988-02-20 | 1988-08-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-21 | 1992-02-20 | 1992-08-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-21 | 1996-02-20 | 1996-08-21 | $7700.00 | Window Closed / Lapsed |