US4299815A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carcinoembryonic antigen determination
Assignee: Hoffmann La Roche Inc
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Filing: 1980-02-08
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Granted: 1981-11-10
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Expires: 2000-02-08
Abstract
Abstract An improved process for the determination of carcinoembryonic antigen in a sample of serum or plasma is disclosed. The improved process, in essence, comprises negating potentially interfering substances in the sample by diluting the sample with water and heating to a temperature below that which, at the ionic strength and pH of the sample, would cause coagulation of the proteins present and carrying out a radioimmunoassay for carcinoembryonic antigen on said sample.
Inventors
- 👤 Hans J. Hansen
- 👤 Alfred D. Myl
- 👤 Jacques P. Vandevoorde
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57565; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/825
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
207 words
A method of determining the concentration of carcinoembryonic antigen in a sample of serum or plasma from a human which comprises: (a) adding sufficient water to said sample to dilute it to a dilution of from about 1:8 to about 1:50, (b) heating said diluted sample to a temperature below that which will cause the protein present in said sample to coagulate for a period of from about 3 to about 30 minutes, thus neutralizing materials in said sample which would interfere with said determination; (c) adding an excess of an antibody to CEA to said sample and incubating for a predetermined time; (d) adding to said sample an amount of carcinoembryonic antigen labeled with a labeling substance capable of being quantitatively determined at least sufficient to react with the amount of antibody added in step (c) and incubating for a predetermined time; (e) adding to said sample an insolubilizing agent thus forming a solid phase containing antibody-bound CEA and a liquid phase containing unbound CEA; (f) separating said solid and liquid phases; (g) determining the amount of said labeling substance present in either said solid or said liquid phase; and (h) determining the amount of carcinoembryonic antigen present in said sample by comparison against a standard.
Claim 2
Independent Claim
207 words
A method of determining the concentration of carcinoembryonic antigen in a sample of serum or plasma from a human which comprises: (a) adding sufficient water to said sample to dilute it to a dilution of from about 1:8 to about 1:50, (b) heating said diluted sample to a temperature below that which will cause the protein present in said sample to coagulate for a period of from about 3 to about 30 minutes, thus neutralizing materials in said sample which would interfere with said determination; (c) adding an excess of an antibody to CEA to said sample and incubating for a predetermined time; (d) adding to said sample an amount of carcinoembryonic antigen labeled with a labeling substance capable of being quantitatively determined at least sufficient to react with the amount of antibody added in step (c) and incubating for a predetermined time; (e) adding to said sample an insolubilizing agent thus forming a solid phase containing antibody-bound CEA and a liquid phase containing unbound CEA; (f) separating said solid and liquid phases; (g) determining the amount of said labeling substance present in either said solid or said liquid phase; and (h) determining the amount of carcinoembryonic antigen present in said sample by comparison against a standard.
Claim 3
Dependent Claim
27 words
The method in accordance with claim 1 wherein said insolubilizing agent is an antibody against the antibody in step (c), said second antibody being in insolubilized form.
Claim 4
Dependent Claim
18 words
The method in accordance with claim 1 wherein said sample is diluted to a dilution of about 1:16.
Claim 5
Dependent Claim
24 words
The method in accordance with claim 1 wherein said diluted sample is heated for a period of from about 5 to about 10 minutes.
Claim 6
Dependent Claim
24 words
The method in accordance with claim 1 wherein said diluted sample is heated to a temperature between about 85° C. and about 105° C.
Claim 7
Dependent Claim
19 words
The method in accordance with claim 5 wherein said sample is heated to a temperature of about 95° C.
Claim 8
Dependent Claim
37 words
The method in accordance with claim 1 wherein said water utilized to dilute said sample contains a sufficient amount of a suitable buffer to buffer said sample to a pH of from about 7.5 to about 9.
Claim 9
Dependent Claim
14 words
The method in accordance with claim 1 wherein said labeling substance is a radioisotope.
Claim 10
Dependent Claim
15 words
The method in accordance with claim 8 wherein said radioisotope is a radioisotope of iodine.
Claim 11
Dependent Claim
14 words
The method in accordance with claim 9 wherein said radioisotope of iodine is iodine-125.
Claim 12
Dependent Claim
14 words
The method in accordance with claim 1 wherein said labeling substance is an enzyme.
Claim 13
Dependent Claim
15 words
The method in accordance with claim 1 wherein said labeling substance is a fluorescent substance.
Claim 14
Dependent Claim
15 words
The method in accordance with claim 1 wherein said labeling substance is a chemiluminescent substance.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-09 | 1985-05-11 | 1985-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-09 | 1989-05-11 | 1989-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-09 | 1993-05-11 | 1993-11-10 | $7700.00 | Window Closed / Lapsed |