US4370264A
Expired / Lapsed
US
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Method for the cold sterilization of preparations containing blood coagulation factor VIII
Assignee: Biotest AG
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Filing: 1981-09-08
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Granted: 1983-01-25
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Expires: 2001-09-08
Abstract
Abstract A process for the cold sterilization of a preparation containing blood coagulation factor VIII, comprising (A) contacting a protein solution containing factor VIII with a physiologically compatible, non-ionogenic tenside, and then subjecting the solution to (B) irradiation with ultraviolet light, (C) treatment with beta-propiolactione, and (D) to an adsorption treatment with colloidal silica, steps B-C-D being performed in the sequence B-C-D, B-D-C, D-C-B or D-B-C.
Inventors
- 👤 Ronald Kotitschke
- 👤 Wolfgang Stephan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:32
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/16; A61L2/20; A61L2/02; A61L2/08; A61L2/10; A61L2103/00; A61L2103/05; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
65 words
A process for the cold sterilization of a preparation containing blood coagulation factor VIII, comprising (A) contacting a protein solution containing factor VIII with a physiologically compatible, non-ionogenic tenside, and then subjecting the solution to (B) irradiation with ultraviolet light, (C) treatment with beta-propiolactone, and (D) to an adsorption treatment with colloidal silica, steps B-C-D being performed in the sequence B-C-D, B-D-C, D-C-B or D-B-C.
Claim 2
Dependent Claim
65 words
A process for the cold sterilization of a preparation containing blood coagulation factor VIII, comprising (A) contacting a protein solution containing factor VIII with a physiologically compatible, non-ionogenic tenside, and then subjecting the solution to (B) irradiation with ultraviolet light, (C) treatment with beta-propiolactone, and (D) to an adsorption treatment with colloidal silica, steps B-C-D being performed in the sequence B-C-D, B-D-C, D-C-B or D-B-C.
Claim 3
Dependent Claim
32 words
A process according to claim 1, wherein the treatments are carried out in the sequence B-C-D, and the solution after irradiation with ultraviolet light is fractionated before it is treated with beta-propiolactone.
Claim 4
Dependent Claim
30 words
A process according to claim 1, wherein the physiologically compatible non-iogenic tenside is a sorbitan fatty acid ester or polyoxyethylene derivative thereof in a concentration of about 1.0 to 5%.
Claim 5
Dependent Claim
20 words
A process according to claim 1, wherein the ultraviolet light is of an intensity of about 2 mWatt/cm 2 ×min.
Claim 6
Dependent Claim
24 words
A process according to claim 1, wherein the beta-propiolactone is used in a concentration of about 0.012 to 0.067 g per g of protein.
Claim 7
Dependent Claim
38 words
A process according to claim 1, wherein the colloidal silica has a specific surface area of about 50 to 400 m 2 and is used in a concentration of about 1 to 3 g per g of protein.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-24 | 1986-07-26 | 1987-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-24 | 1990-07-26 | 1991-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-24 | 1994-07-26 | 1995-01-25 | $7700.00 | Window Closed / Lapsed |