Ion exchanger treatment of citrate-stabilized plasma

Claim 1

In the fractionation of blood comprising the steps of taking blood from a donor, separating the solids from the plasma, freezing the plasma, thawing the frozen plasma and separating from the thawed plasma fluid a first product comprising undissolved cryoprecipitate enriched in factor-VIII protein; dissolving the first product, removing impurities from the solution of the first product, precipitating the factor-VIII protein from the solution; after separation of the first product treating the residual thawed plasma fluid with a solid adsorbent thereby to adsorb the factors II, VII, IX and X, and separating a second product comprising solid adsorbent and the adsorbed factors from the residual plasma; and, after separation of the second product, contacting the residual plasma with colloidal silica and separating a third product comprising a storage-stable serum protein solution from the colloidal silica, the improvement which comprises mixing the blood or plasma with a citrate stabilizer prior to freezing and treating the residual thawed plasma fluid with an anion exchange resin and a cation exchange resin prior to treatment with the solid adsorbent.