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Patent US4359535A successfully refreshed from Google Patents.
US4359535A
Expired / Lapsed
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Autonomously replicating DNA containing inserted DNA sequences
Assignee: Individual
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Filing: 1979-10-01
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Granted: 1982-11-16
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Expires: 1999-10-01
Abstract
Abstract Autonomously replicating DNA containing a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonuclease cleavage.
Inventors
- 👤 George Pieczenik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:52
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/102
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
51 words
A population of linear DNA molecules of an autonomously replicating DNA element, randomly cleaved, comprising circular permutations of the same nucleotide sequence, to the ends of which have been covalently attached an oligonucleotide of unique sequence, being an oligonucleotide of which its sequence does not otherwise exist in said DNA element.
Claim 2
Dependent Claim
51 words
A population of linear DNA molecules of an autonomously replicating DNA element, randomly cleaved, comprising circular permutations of the same nucleotide sequence, to the ends of which have been covalently attached an oligonucleotide of unique sequence, being an oligonucleotide of which its sequence does not otherwise exist in said DNA element.
Claim 3
Dependent Claim
41 words
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferable DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonuclease cleavage.
Claim 4
Dependent Claim
41 words
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferable DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonuclease cleavage.
Claim 5
Dependent Claim
41 words
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferrable DNA inserted in a structural gene thereof at a site not previously susceptible of restriction endonuclease cleavage.
Claim 6
Dependent Claim
41 words
An autonomously replicating, transferable DNA comprising a unique nucleotide sequence, being an oligonucleotide of which its sequence does not otherwise exist in said transferrable DNA inserted in a structural gene thereof at a site not previously susceptible of restriction endonuclease cleavage.
Claim 7
Dependent Claim
15 words
A DNA according to claim 2 or 3 having more than one inserted oligonucleotide sequence.
Claim 8
Dependent Claim
18 words
A DNA according to claim 2 or 3 wherein the unique oligonucleotide sequence comprises a unique restriction site.
Claim 9
Dependent Claim
15 words
A DNA according to claim 5 comprising heterologous DNA inserted at said unique restriction site.
Claim 10
Dependent Claim
28 words
A DNA according to claim 3 wherein the unique oligonucleotide sequence comprises a unique restriction site, wherein heterologous DNA is inserted such that said heterologous DNA is expressible.
Claim 11
Dependent Claim
44 words
A DNA according to claim 7 wherein said heterologous DNA is rendered expressible by addition of 0, 1 or 2 nucleotides to said unique oligonucleotide sequence, whereby any coding sequence present in said heterologous DNA is in reading frame phase with said structural gene.
Claim 12
Dependent Claim
29 words
A DNA according to claim 2 wherein the unique oligonucleotide sequence comprises a unique restriction site having inserted therein heterologous DNA such that said heterologous DNA is not expressible.
Claim 13
Dependent Claim
66 words
A sequencing vector comprising an autonomously replicating DNA element having an insert comprising a unique restriction site, being a restriction site of which its sequence does not otherwise exist in the DNA element, adjacent to a unique nucleotide sequence of which its sequence does not otherwise exist in the DNA element, said unique restriction site being located on the 5' side of said unique nucleotide sequence.
Claim 14
Dependent Claim
66 words
A sequencing vector comprising an autonomously replicating DNA element having an insert comprising a unique restriction site, being a restriction site of which its sequence does not otherwise exist in the DNA element, adjacent to a unique nucleotide sequence of which its sequence does not otherwise exist in the DNA element, said unique restriction site being located on the 5' side of said unique nucleotide sequence.
Claim 15
Dependent Claim
22 words
A sequencing vector according to claim 10, comprising a MIRV having a plurality of unique restriction sites adjacent to unique nucleotide sequences.
Claim 16
Dependent Claim
29 words
A sequencing vector according to claim 10, comprising additionally a population of linear DNA molecules comprising circular permutations of the same nucleotide sequence, inserted in said unique restriction site.
Claim 17
Dependent Claim
20 words
A sequencing vector according to claim 11, comprising additionally a nucleotide sequence inserted at one of said unique restriction sites.
References Cited (Backward Citations)
US4237224A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-15 | 1986-05-17 | 1986-11-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-15 | 1990-05-17 | 1990-11-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-15 | 1994-05-17 | 1994-11-16 | $7700.00 | Window Closed / Lapsed |