Method and apparatus for analyzing contaminants in aqueous humor

Claim 1

An apparatus for measuring contaminants in humor of an eye by non-invasive measurement of the eye, the apparatus comprising: (a) a continuous wave laser for producing light beams of known wave length; (b) projection means for directing and converging a light beam from the laser to a focal point in the humor, said projection means including a gonioscopic lens having a transparent housing with an opaque outer surface and an attached concave recess adapted to fit over the cornea of the examined eye, wherein the concave recess is substantially opaque except for a transparent entrance port to admit the laser beam into the humor, a transparent viewing port to permit a clinician to view scattering of the beam in the humor, and a transparent exit port to collect scattered light from the humor; and (c) means for analyzing the composition of macromolecular contaminants in the humor by analyzing the diffusion constant of each contaminant by using the light scattered from the focal point at a particular scattering angle θ relative to a line of symmetry for the beam in the humor.

Claim 2

An apparatus for measuring contaminants in humor of an eye by non-invasive measurement of the eye, the apparatus comprising: (a) a continuous wave laser for producing light beams of known wave length; (b) projection means for directing and converging a light beam from the laser to a focal point in the humor, said projection means including a gonioscopic lens having a transparent housing with an opaque outer surface and an attached concave recess adapted to fit over the cornea of the examined eye, wherein the concave recess is substantially opaque except for a transparent entrance port to admit the laser beam into the humor, a transparent viewing port to permit a clinician to view scattering of the beam in the humor, and a transparent exit port to collect scattered light from the humor; and (c) means for analyzing the composition of macromolecular contaminants in the humor by analyzing the diffusion constant of each contaminant by using the light scattered from the focal point at a particular scattering angle θ relative to a line of symmetry for the beam in the humor.

Claim 6

An apparatus for measuring contaminants in humor of an eye by non-invasive measurement of the eye, the apparatus comprising: (a) a continuous wave laser for producing light beams of known wave length; (b) projection means for directing and converging a light beam from the laser to a focal point in the humor; (c) means for analyzing the composition of macromolecular contaminants in the humor by measuring the diffusion constant of each macromolecular contaminant by using the light scattered from the focal point at a particular scattering angle θ relative to a line of symmetry for the beam in the aqueous humor; (d) wherein the projection means comprises a gonioscopic device having: (i) a housing having an input mirror for directing the beam into the humor; (ii) said housing having an attached concave recess adapted to fit over the cornea of the examined eye, wherein the recess is substantially opaque except for a plurality of transparent ports, one of said ports being an entrance port to admit the beam relected from the input mirror into the humor, and another of said ports being an exit port to collect scattered light from the humor; and (iii) said housing having an output mirror for directing the scattered light to the analyzing means.

Claim 7

An apparatus for measuring contaminants in humor of an eye by non-invasive measurement of the eye, the apparatus comprising: (a) a continuous wave laser for producing light beams of known wave length; (b) projection means for directing and converging a light beam from the laser to a focal point in the humor; (c) means for analyzing the composition of macromolecular contaminants in the humor by measuring the diffusion constant of each macromolecular contaminant by using the light scattered from the focal point at a particular scattering angle θ relative to a line of symmetry for the beam in the aqueous humor; (d) wherein the projection means comprises a gonioscopic device having: (i) a housing having an input mirror for directing the beam into the humor; (ii) said housing having an attached concave recess adapted to fit over the cornea of the examined eye, wherein the recess is substantially opaque except for a plurality of transparent ports, one of said ports being an entrance port to admit the beam relected from the input mirror into the humor, and another of said ports being an exit port to collect scattered light from the humor; and (iii) said housing having an output mirror for directing the scattered light to the analyzing means.

Claim 10

A method for analyzing the composition of contaminants in aqueous humor by non-invasive measurement of an examined eye, the method comprising: (a) directing and focusing a laser beam of known wavelength through a gonioscopic lens to a focal point in the aqueous humor; (b) collecting light scattered from the focal point at a scattering angle θ relative to a line of symmetry for the beam in the aqueous humor; (c) transforming the collected light into electrical pulses; (d) producing electrical signals representative of the following autocorrelation function G(kΔt) for only those pulses above a predetermined amplitude: ##EQU5## where: Δt=a predetermined time interval; n=the number of pulses during a particular time interval; i=an indexing number going from 1 to the total number of intervals X the scattered light is collected; and K=a plurality of successive integers starting with the integer 1; (e) computing the composition of contaminants in the aqueous humor by using standard curve fitting routines for the following alternative equation for the autocorrelation function G(KΔt) to solve for B 1 , B 2 . . . B n and D 1 , D 2 . . . D n : G(KΔt)=A'+[B.sub.1 e.sup.-D.sbsb.1.sup.C.spsp.2.sup.(KΔt) +B.sub.2 e.sup.-D.sbsb.2.sup.C.spsp.2.sup.(KΔt) + . . . B.sub.n e.sup.-D.sbsb.n.sup.C.spsp.2.sup.(KΔt) ].sup.2 where: A'=a constant; B 1 , B 2 . . . B n =constants each dependent upon the concentration of a particular type of contaminant in the aqueous humor; D 1 , D 2 , D n =diffusion constants for each potential contaminant; and e=the base of natural logarithms; wherein known diffusion constants for contaminants are substituted for D 1 , D 2 . . . D n and B 1 , B 2 . . . B n are assigned different values until the values of the alternative equation best approximate the measured electrical signals.

Claim 11

A method for analyzing the composition of contaminants in aqueous humor by non-invasive measurement of an examined eye, the method comprising: (a) directing and focusing a laser beam of known wavelength through a gonioscopic lens to a focal point in the aqueous humor; (b) collecting light scattered from the focal point at a scattering angle θ relative to a line of symmetry for the beam in the aqueous humor; (c) transforming the collected light into electrical pulses; (d) producing electrical signals representative of the following autocorrelation function G(kΔt) for only those pulses above a predetermined amplitude: ##EQU5## where: Δt=a predetermined time interval; n=the number of pulses during a particular time interval; i=an indexing number going from 1 to the total number of intervals X the scattered light is collected; and K=a plurality of successive integers starting with the integer 1; (e) computing the composition of contaminants in the aqueous humor by using standard curve fitting routines for the following alternative equation for the autocorrelation function G(KΔt) to solve for B 1 , B 2 . . . B n and D 1 , D 2 . . . D n : G(KΔt)=A'+[B.sub.1 e.sup.-D.sbsb.1.sup.C.spsp.2.sup.(KΔt) +B.sub.2 e.sup.-D.sbsb.2.sup.C.spsp.2.sup.(KΔt) + . . . B.sub.n e.sup.-D.sbsb.n.sup.C.spsp.2.sup.(KΔt) ].sup.2 where: A'=a constant; B 1 , B 2 . . . B n =constants each dependent upon the concentration of a particular type of contaminant in the aqueous humor; D 1 , D 2 , D n =diffusion constants for each potential contaminant; and e=the base of natural logarithms; wherein known diffusion constants for contaminants are substituted for D 1 , D 2 . . . D n and B 1 , B 2 . . . B n are assigned different values until the values of the alternative equation best approximate the measured electrical signals.