Patent US4342828A successfully refreshed from Google Patents.
US4342828A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for producing substance capable of stimulating differentiation and proliferation of human granulopoietic stem cells
Assignee: Morinaga Milk Industry Co Ltd
•
Filing: 1980-07-15
•
Granted: 1982-08-03
•
Expires: 2000-07-15
Abstract
Abstract A colony stimulating factor effective in treating human granulocytopenia is produced by cultivating monocytes and macrophages isolated from the human peripheral blood in a synthetic medium for tissue culture containing a glycoprotein isolated from human urine and capable of stimulating the formation of human granulocytes or mouse macrophages and granulocytes.
Inventors
- 👤 Fumimaro Takaku
- 👤 Katsuhiro Ogasa
- 👤 Morio Kuboyama
- 👤 Nobuya Yanai
- 👤 Muneo Yamada
- 👤 Yoshiteru Watanabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:35
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/38; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/53; C07K14/535; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
94 words
A method for producing a substance capable of stimulating the proliferation and differentiation of human granulopoietic stem cells, which comprises cultivating monocytes and macrophages, which have been separated from the human peripheral blood, in a synthetic medium for tissue culture containing a glycoprotein separated from the human urine and capable of stimulating human or mouse bone marrow cells to form human granulocytes or mouse macrophages and granulocytes, thereby producing a substance capable of stimulating the proliferation and differentiation of human granulopoietic stem cells in the medium, and recovering the active substance from the medium.
Claim 2
Independent Claim
94 words
A method for producing a substance capable of stimulating the proliferation and differentiation of human granulopoietic stem cells, which comprises cultivating monocytes and macrophages, which have been separated from the human peripheral blood, in a synthetic medium for tissue culture containing a glycoprotein separated from the human urine and capable of stimulating human or mouse bone marrow cells to form human granulocytes or mouse macrophages and granulocytes, thereby producing a substance capable of stimulating the proliferation and differentiation of human granulopoietic stem cells in the medium, and recovering the active substance from the medium.
Claim 3
Dependent Claim
23 words
A method according to claim 1, wherein the glycoprotein is a glycoprotein capable of stimulating human bone marrow cells to form human granulocytes.
Claim 4
Dependent Claim
25 words
A method according to claim 1, wherein the glycoprotein is a glycoprotein capable of stimulating human bone marrow cells to form mouse macrophages and granulocytes.
Claim 5
Dependent Claim
17 words
A method according to claim 1, wherein the cultivation is carried out in the presence of serum.
Claim 6
Dependent Claim
16 words
A method according to claim 4, wherein the serum present in the medium is human serum.
Claim 7
Dependent Claim
23 words
A method according to claim 5, wherein the amount of the serum is at least 5% based on the volume of the medium.
Claim 8
Dependent Claim
23 words
A method according to claim 2, wherein the glycoprotein content of the medium is at least 0.1 μg per ml of the medium.
Claim 9
Dependent Claim
23 words
A method according to claim 3, wherein the glycoprotein content of the medium is at least 500 units per ml of the medium.
Claim 10
Dependent Claim
34 words
A method for producing a substance capable of stimulating the proliferation and differentiation of human granulopoietic stem cells according to claim 1, wherein the glycoprotein is a partially purified material accompanied with urinary proteins.
Claim 11
Dependent Claim
29 words
A method according to claim 1, wherein the number of cells of monocytes and macrophages inoculated into the medium is at least 10 5 per ml of the medium.
References Cited (Backward Citations)
US4135975A
Cited
US4275056A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-08-02 | 1986-02-01 | 1986-08-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-08-02 | 1990-02-01 | 1990-08-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-08-02 | 1994-02-01 | 1994-08-03 | $7700.00 | Window Closed / Lapsed |