US3970518A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Magnetic separation of biological particles
Assignee: General Electric Co
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Filing: 1975-07-01
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Granted: 1976-07-20
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Expires: 1995-07-01
Abstract
Abstract Small magnetic particles coated with an antibody layer are used to provide large and widely-distributed surface area for sorting out and separating select viruses, bacteria and other cells from multi-cell, bacteria or virus populations.
Inventors
- 👤 Ivar Giaever
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; B; B03; B03C; B03C1/00; B03C1/005; B03C1/01; C; C12; C12N; C12N1/00; C12N1/02; C12N7/00; C12N2710/00; C12N2710/00011; C12N2710/00051
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
136 words
A method for the separation of a select population of cells, bacteria or viruses from a mixed population comprising the steps of: contacting a solution of said mixed population containing members of said select population with a plurality of small magnetic particles each of which is coated with a layer of antibodies to the select population bonded thereto, magnetically separating said magnetic particles from said solution, said magnetic particles having on surface area thereof members of said select population affixed as a complex to said layer of antibodies, contacting these coated magnetic particles with said members affixed thereto with a cleaving agent solution to detach said members of the select populaton from said antibody-coated magnetic particles and magnetically separating the antibody-coated magnetic particles from the cleaving agent solution now containing said members of the select population.
Claim 2
Independent Claim
136 words
A method for the separation of a select population of cells, bacteria or viruses from a mixed population comprising the steps of: contacting a solution of said mixed population containing members of said select population with a plurality of small magnetic particles each of which is coated with a layer of antibodies to the select population bonded thereto, magnetically separating said magnetic particles from said solution, said magnetic particles having on surface area thereof members of said select population affixed as a complex to said layer of antibodies, contacting these coated magnetic particles with said members affixed thereto with a cleaving agent solution to detach said members of the select populaton from said antibody-coated magnetic particles and magnetically separating the antibody-coated magnetic particles from the cleaving agent solution now containing said members of the select population.
Claim 3
Dependent Claim
22 words
The separation method recited in claim 1 wherein the magnetic particles are selected from the group consisting of ferromagnetic and ferrimagnetic materials.
Claim 4
Dependent Claim
29 words
The separation method recited in claim 1 wherein after separation thereof from the solution the magnetic particles are held by means of an electrically induced magnetic field and washed.
Claim 5
Dependent Claim
16 words
The separation method recited in claim 1 wherein the cleaving agent is a weak acid solution.
Claim 6
Dependent Claim
13 words
The separation method recited in claim 4 wherein the acid is citric acid.
Claim 7
Dependent Claim
14 words
The separation method recited in claim 1 wherein the select populatin is of cells.
Claim 8
Dependent Claim
13 words
The separation method recited in claim 1 wherein the select population is bacterial.
Claim 9
Dependent Claim
13 words
The separation method recited in claim 1 wherein the select population is viral.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-07-20 | 1980-01-19 | 1980-07-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-07-20 | 1984-01-19 | 1984-07-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-07-20 | 1988-01-19 | 1988-07-20 | $7700.00 | Window Closed / Lapsed |