US4289850A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Interferon production
Assignee: Beecham Group PLC
•
Filing: 1980-01-10
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Granted: 1981-09-15
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Expires: 2000-01-10
Abstract
Abstract A method for preparing interferon in which MRC-5 cells are induced for 2 to 3 hours with an interferon-inducing medium containing from 0.1 to 50 mg 1 -1 of a ds-RNA, 100 mg 1 -1 of DEAE-Dextran and 250 m.M of sucrose. Other sugars which can be used include glucose, galactose, fructose, mannose and maltose, and a preferred ds-RNA is naturally occurring ds-RNA isolated from P. chrysogenum.
Inventors
- 👤 Jeffery H. Robinson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:38
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C12; C12N; C12N1/00; C12P; C12P21/00; C12R; C12R2001/00; C12R2001/91; Y; Y10; Y10S; Y10S435/00; Y10S435/811; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
63 words
A method for preparing interferon, in which interferon-producing cells are contacted in vitro with an interferon-inducing medium comprising a non-toxic quantity of an interferon-inducing double-stranded polynucleotide, diethylamine ethyl dextran (DEAE-Dextran), and at least 100 m.mol 1 -1 of a water soluble sugar in a physiologically acceptable aqueous vehicle at physiological conditions of temperature and pH, and the interferon so formed is thereafter separated.
Claim 2
Independent Claim
63 words
A method for preparing interferon, in which interferon-producing cells are contacted in vitro with an interferon-inducing medium comprising a non-toxic quantity of an interferon-inducing double-stranded polynucleotide, diethylamine ethyl dextran (DEAE-Dextran), and at least 100 m.mol 1 -1 of a water soluble sugar in a physiologically acceptable aqueous vehicle at physiological conditions of temperature and pH, and the interferon so formed is thereafter separated.
Claim 3
Dependent Claim
21 words
A method according to claim 1, wherein the amount of sugar present is from 200 to 500 m.mol 1 -1 .
Claim 4
Dependent Claim
17 words
A method according to claim 1, wherein the sugar is glucose, galactose, sucrose, fructose, mannose or maltose.
Claim 5
Dependent Claim
15 words
A method according to claim 1, wherein the double-stranded polynucleotide is a naturally occurring ds-RNA.
Claim 6
Dependent Claim
15 words
A method according to claim 4, characterised in that the ds-RNA is isolated from P-chrysogenum.
Claim 7
Dependent Claim
26 words
A method according to claim 1, wherein the concentration of the double-stranded polynucleotide is in the range of from 0.1 to 50 mg 1 -1 .
Claim 8
Dependent Claim
24 words
A method according to claim 1, wherein the concentration of DEAE-Dextran in the inducing medium is from 100 to 300 mg 1 -1 .
Claim 9
Dependent Claim
26 words
A method according to claim 1, wherein an appropriate quantity of cells is first cultured, and thereafter the cultured cells are treated with the interferon-inducing medium.
Claim 10
Dependent Claim
17 words
A method according to claim 8, wherein the cells are leucocytes, epithelial cells, fibroblasts or diploid fibroblasts.
References Cited (Backward Citations)
US4007086A
Cited
US4144126A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-14 | 1985-03-16 | 1985-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-14 | 1989-03-16 | 1989-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-14 | 1993-03-16 | 1993-09-15 | $7700.00 | Window Closed / Lapsed |