US4007086A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Interferon induction
Assignee: Pharmacia and Upjohn Co
•
Filing: 1975-01-23
•
Granted: 1977-02-08
•
Expires: 1995-01-23
Abstract
Abstract An improved method for producing interferon which comprises irradiating interferon-producing human or animal cells in vitro with ultraviolet irradiation.
Inventors
- 👤 Ramon D. Hamilton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:21
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
78 words
In the method of producing interferon in vitro which consists of inducing interferon-producing human or animal cells with an inducing-effective amount of a non-viral interferon inducer, the improvement comprising irradiating the cells with from about 50 ergs/mm 2 to about 2500 ergs/mm 2 of ultraviolet irradiation applied at a point in time during the time span of from 8 hours prior to until about 4 hours subsequent to, including concurrent with, the addition of the non-viral interferon inducer.
Claim 2
Dependent Claim
78 words
In the method of producing interferon in vitro which consists of inducing interferon-producing human or animal cells with an inducing-effective amount of a non-viral interferon inducer, the improvement comprising irradiating the cells with from about 50 ergs/mm 2 to about 2500 ergs/mm 2 of ultraviolet irradiation applied at a point in time during the time span of from 8 hours prior to until about 4 hours subsequent to, including concurrent with, the addition of the non-viral interferon inducer.
Claim 3
Dependent Claim
14 words
The method of claim 1 in which the non-viral interferon inducer is a polynucleotide.
Claim 4
Dependent Claim
13 words
The method of claim 2 in which the polynucleotide is polyriboinosinic-polyribocytidylic acid complex.
Claim 5
Dependent Claim
20 words
The method of claim 3 in which the cells are irradiated subsequent to the addition of the polyriboinosinic-polyribocytidylic acid complex.
Claim 6
Dependent Claim
23 words
The method of claim 4 in which the cells are irradiated with from about 100 ergs/mm 2 to about 1000 ergs/mm 2 .
Claim 7
Dependent Claim
13 words
The method of claim 5 in which the cells are human fibroblast cells.
Claim 8
Dependent Claim
13 words
The method of claim 5 in which the cells are rabbit kidney cells.
Claim 9
Dependent Claim
16 words
The method of claim 6 in which the human fibroblast cells are human foreskin fibroblast cells.
Claim 10
Dependent Claim
20 words
The method of claim 3 in which the cells are irradiated concurrent with the addition of the polyriboinosinic-polyribocytidylic acid complex.
Claim 11
Dependent Claim
23 words
The method of claim 9 in which the cells are irradiated with from about 100 ergs/mm 2 to about 1000 ergs/mm 2 .
Claim 12
Dependent Claim
13 words
The method of claim 10 in which the cells are human fibroblast cells.
Claim 13
Dependent Claim
13 words
The method of claim 10 in which the cells are rabbit kidney cells.
Claim 14
Dependent Claim
16 words
The method of claim 11 in which the human fibroblast cells are human foreskin fibroblast cells.
Claim 15
Dependent Claim
20 words
The method of claim 3 in which the cells are irradiated prior to the addition of the polyriboinosinic-polyribocytidylic acid complex.
Claim 16
Dependent Claim
23 words
The method of claim 14 in which the cells are irradiated with from about 100 ergs/mm 2 to about 1000 ergs/mm 2 .
Claim 17
Dependent Claim
13 words
The method of claim 15 in which the cells are human fibroblast cells.
Claim 18
Dependent Claim
13 words
The method of claim 15 in which the cells are rabbit kidney cells.
Claim 19
Dependent Claim
16 words
The method of claim 16 in which the human fibroblast cells are human foreskin fibroblast cells.
Claim 20
Dependent Claim
23 words
The method of claim 1 in which the cells are irradiated with from about 100 ergs/mm 2 to about 1000 ergs/mm 2 .
Claim 21
Dependent Claim
36 words
The method of claim 1 in which the cells are irradiated at a point in time during the time span of immediately prior to until 2 hours subsequent to the addition of the non-viral interferon inducer.
Claim 22
Dependent Claim
23 words
The method of claim 20 in which the cells are irradiated with from about 100 ergs/mm 2 to about 1000 ergs/mm 2 .
Claim 23
Dependent Claim
15 words
The method of claim 21 in which the non-viral interferon inducer is polyriboinosinic-polyribocytidylic acid complex.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-08 | 1980-08-09 | 1981-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-08 | 1984-08-09 | 1985-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-08 | 1988-08-09 | 1989-02-08 | $7700.00 | Window Closed / Lapsed |