US3953290A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Polypeptides as vitro active cell growth enhancing factors and methods of use
Assignee: Phadia AB
•
Filing: 1973-04-30
•
Granted: 1976-04-27
•
Expires: 1993-04-30
Abstract
Abstract Disclosed are in vitro active cell growth factors that stimulate deoxyribonucleic acid synthesis in the cells, are polypeptide in chemical structure, having a molecular weight range of from 4,000 to 6,000 and an isoelectric range of from pH 4.3 to 5.3, are inert to about 72% aqueous ethanol containing by volume about 0.047% of concentrated hydrochloric acid, soluble in and precipitated from such acid aqueous ethanol solution by admixing in it 3.5 times its volume of a mixture of five parts of acetone with three parts of ethanol, and are soluble in about twice their weight of 20% formic acid.
Inventors
- 👤 Knut Oivind Uthne
- 👤 Gustaf Bertil Aberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:31
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
155 words
In vitro active cell growth factors derivable from animal blood plasma or serum, or the Cohn et al. precipitate IV or solution IV-6 + 7, and characterized in that they i. stimulate deoxyribonucleic acid synthesis in the cells, ii. are polypeptide in chemical structure, iii. have a molecular weight range of from 4,000 to 6,000 and iv. an isoelectric range of from pH 4.3 to 5.3, v. are soluble in and otherwise inert to about 72% aqueous ethanol containing by volume about 0.047% of concentrated hydrochloric acid, vi. remain soluble in that aqueous ethanol after it is adjusted with 4M sodium hydroxide to pH 8.4, vii. are precipitated from the resulting pH 8.4 aqueous ethanol solution by admixing that ethanol solution of pH 8.4 with 3.5 times its volume of a mixture of 5 parts of acetone with 3 parts of ethanol, and viii. are soluble in about twice their weight of 20% formic acid.
Claim 2
Dependent Claim
155 words
In vitro active cell growth factors derivable from animal blood plasma or serum, or the Cohn et al. precipitate IV or solution IV-6 + 7, and characterized in that they i. stimulate deoxyribonucleic acid synthesis in the cells, ii. are polypeptide in chemical structure, iii. have a molecular weight range of from 4,000 to 6,000 and iv. an isoelectric range of from pH 4.3 to 5.3, v. are soluble in and otherwise inert to about 72% aqueous ethanol containing by volume about 0.047% of concentrated hydrochloric acid, vi. remain soluble in that aqueous ethanol after it is adjusted with 4M sodium hydroxide to pH 8.4, vii. are precipitated from the resulting pH 8.4 aqueous ethanol solution by admixing that ethanol solution of pH 8.4 with 3.5 times its volume of a mixture of 5 parts of acetone with 3 parts of ethanol, and viii. are soluble in about twice their weight of 20% formic acid.
Claim 3
Dependent Claim
14 words
Cell growth factors as claimed in claim 1, wherein said animal is a human.
Claim 4
Dependent Claim
15 words
Cell growth factors as claimed in claim 1, wherein said animal is an equine animal.
Claim 5
Dependent Claim
15 words
Cell growth factors as claimed in claim 1, wherein said animal is a bovine animal.
Claim 6
Dependent Claim
15 words
Cell growth factors as claimed in claim 1, characterized by being derived from blood plasma.
Claim 7
Dependent Claim
18 words
Cell growth factors as claimed in claim 1, characterized by being derived from Cohn et al. precipitate IV.
Claim 8
Dependent Claim
45 words
In the in vitro cultivation of tissue cell cultures, the improvement which is the step of enhancing said cultivation by adding to the culture medium a tissue cell growth enhancing effective amount of polypeptide in vitro active cell growth factors as claimed in claim 1.
Claim 9
Dependent Claim
21 words
The improvement in tissue cell cultivation as claimed in claim 7, wherein the plasma or serum is derived from human blood.
Claim 10
Dependent Claim
22 words
The improvement in tissue cell cultivation as claimed in claim 7, wherein the plasma or serum is derived from equine animal blood.
Claim 11
Dependent Claim
22 words
The improvement in tissue cell cultivation as claimed in claim 7, wherein the plasma or serum is derived from bovine animal blood.
Claim 12
Dependent Claim
19 words
The improvement in tissue cell cultivation as claimed in claim 7, wherein said growth factors are derived from plasma.
Claim 13
Dependent Claim
24 words
The improvement in tissue cell cultivation as claimed in claim 7, wherein said growth factors are derived from the Cohn et al. precipitate IV.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-27 | 1979-10-27 | 1980-04-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-27 | 1983-10-27 | 1984-04-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-27 | 1987-10-27 | 1988-04-27 | $7700.00 | Window Closed / Lapsed |