Patent US4255385A successfully refreshed from Google Patents.
US4255385A
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Reagent and test kit for determining glycosylated hemoglobin
Assignee: Abbott Laboratories
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Filing: 1979-10-23
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Granted: 1981-03-10
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Expires: 1999-10-23
Abstract
Abstract A method, reagent and test kit for determining glycosylated hemoglobin in blood samples which involves liberating hemoglobins from red blood cells by chemical or physical means and reacting non-glycosylated hemoglobin with an allosteric site binding substance which reacts with the allosteric binding site of non-glycosylated hemoglobin and thereby alters the distribution between allosteric forms of the hemoglobins and measuring the change. This method is useful in monitoring glucose metabolism for detecting and controlling diabetes.
Inventors
- 👤 Stephen D. Stroupe
- 👤 Edwin G. Moore
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/723
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
49 words
A test kit for determining glycosylated hemoglobin in blood samples comprising reagent containers holding separate or in combination a red blood cell lysing agent, an oxidizing agent for oxidizing hemoglobin to methemoglobin, a heme-binding ligand, an allosteric site binding substance which binds to the allosteric site of non-glycosylated hemoglobin.
Claim 2
Dependent Claim
49 words
A test kit for determining glycosylated hemoglobin in blood samples comprising reagent containers holding separate or in combination a red blood cell lysing agent, an oxidizing agent for oxidizing hemoglobin to methemoglobin, a heme-binding ligand, an allosteric site binding substance which binds to the allosteric site of non-glycosylated hemoglobin.
Claim 3
Dependent Claim
14 words
The test kit according to claim 1 further containing glycosylated hemoglobin standards or controls.
Claim 4
Dependent Claim
41 words
The test kit according to claim 1 containing separate or in combination a detergent as a red blood cell lysing agent; potassium ferricyanide as an oxidizing agent; imidazole as a heme-binding ligand; and inositol hexaphosphate as an allosteric site binding substance.
Claim 5
Dependent Claim
28 words
A reagent comprising: (a) a red blood cell lysing agent; (b) an oxidizing agent for oxidizing hemoglobin to methemoglobin; and (c) a heme-binding ligand, all in a diluent.
Claim 6
Dependent Claim
28 words
A reagent comprising: (a) a red blood cell lysing agent; (b) an oxidizing agent for oxidizing hemoglobin to methemoglobin; and (c) a heme-binding ligand, all in a diluent.
Claim 7
Dependent Claim
34 words
A reagent comprising: (a) a red blood cell lysing agent; (b) an oxidizing agent for oxidizing hemoglobin to methemoglobin; (c) a heme-binding ligand; and (d) an allosteric site binding substance, all in a diluent.
Claim 8
Dependent Claim
34 words
A reagent comprising: (a) a red blood cell lysing agent; (b) an oxidizing agent for oxidizing hemoglobin to methemoglobin; (c) a heme-binding ligand; and (d) an allosteric site binding substance, all in a diluent.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-09 | 1984-09-08 | 1985-03-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-09 | 1988-09-08 | 1989-03-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-09 | 1992-09-08 | 1993-03-10 | $7700.00 | Window Closed / Lapsed |