US4234561A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antigen for early pregnancy test and contraceptive vaccine
Assignee: Research Corp
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Filing: 1978-02-06
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Granted: 1980-11-18
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Expires: 1998-02-06
Abstract
Abstract Antisera suitable for detecting the presence of human chorionic gonadotropin in body fluids by immunoassay are prepared by administering to a host animal an antigen comprising the beta -subunit of human chorionic gonadotropin which has been modified by cleaving and optionally conjugating the thus modified beta -subunit with a protein or hapten capable of enhancing the immunogenetic potency of the antigen. The antigens are also useful for the contraceptive purposes to terminate pregnancy.
Inventors
- 👤 Om P. Bahl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:10
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/0005; A61K39/0006; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; A61K2039/60; A61K2039/6031; A61K2039/6081; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/81; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
138 words
A radioimmunoassay procedure for detecting the presence of hCG in body fluids which comprises contacting said body fluids with a complex comprising radioactively tagged hCG and an antibody; subsequently separating the complex from said body fluid and measuring the radioactive of either said complex or said body fluid wherein said antibody comprises an antiserum generally selective in reactivity to hCG to a distinguishing degree from the luteinizing hormone, which is produced by the process which comprises: isolating the β-subunit of human chorionic gonadotropin, reducing and cleaving six of the intrachain disulfide bonds of said β-subunit; alkylating the thus reduced intrachain disulfide bonds and conjugating said β-subunit with a protein or hapten; isolating said antigen; administering said antigen to a host animal whereby said animal generates an antibody response to said antigen; and extracting said antiserum from said animal.
Claim 2
Dependent Claim
138 words
A radioimmunoassay procedure for detecting the presence of hCG in body fluids which comprises contacting said body fluids with a complex comprising radioactively tagged hCG and an antibody; subsequently separating the complex from said body fluid and measuring the radioactive of either said complex or said body fluid wherein said antibody comprises an antiserum generally selective in reactivity to hCG to a distinguishing degree from the luteinizing hormone, which is produced by the process which comprises: isolating the β-subunit of human chorionic gonadotropin, reducing and cleaving six of the intrachain disulfide bonds of said β-subunit; alkylating the thus reduced intrachain disulfide bonds and conjugating said β-subunit with a protein or hapten; isolating said antigen; administering said antigen to a host animal whereby said animal generates an antibody response to said antigen; and extracting said antiserum from said animal.
Claim 3
Dependent Claim
24 words
The radioimmunoassay of claim 1, wherein said protein or hapten is selected from the group consisting of albumin, hemocyanin, thyroglobulin, and muramyl alanyl isoglutamine.
Claim 4
Dependent Claim
13 words
The radioimmunoassay procedure of claim 2, wherein said protein or hapten is hemocyanin.
Claim 5
Dependent Claim
19 words
A pregnancy test which comprises subjecting a body fluid from a woman to the radioimmunoassay technique of claim 1.
Claim 6
Dependent Claim
25 words
A test for tumors in patients which secrete hCG, which comprises subjecting a body fluid from the patient to the radioimmunoassay technique of claim 1.
Claim 7
Dependent Claim
27 words
A technique for monitoring the progress of a patient's pregnancy, which comprises periodically measuring the hCG level of the patient by the radioimmunoassay technique of claim 1.
Claim 8
Dependent Claim
29 words
A technique for monitoring the progress of chemotherapy of hCG secreting tumors, which comprises periodically measuring the hCG level of the patient by the radioimmunoassay technique of claim 1.
Claim 9
Dependent Claim
105 words
In an immunoassay technique for detecting the presence of hCG in body fluids, the improvement which comprises the antiserum which is generally selective in reactivity to human chorionic gonadotropin to a distinguishing degree from luteinizing hormone, which is produced by the process comprising: isolating the β-subunit of human chorionic gonadotropin, reducing and cleaving six of the intrachain disulfide bonds of said β-subunit, alkylating the thus reduced intrachain disulfide bonds and conjugating said B subunit with protein or hapten, isolating said antigen, administering said antigen to a host animal whereby said animal generates an antibody response to said antigen, and exracting said antiserum from said animal.
Claim 10
Dependent Claim
105 words
In an immunoassay technique for detecting the presence of hCG in body fluids, the improvement which comprises the antiserum which is generally selective in reactivity to human chorionic gonadotropin to a distinguishing degree from luteinizing hormone, which is produced by the process comprising: isolating the β-subunit of human chorionic gonadotropin, reducing and cleaving six of the intrachain disulfide bonds of said β-subunit, alkylating the thus reduced intrachain disulfide bonds and conjugating said B subunit with protein or hapten, isolating said antigen, administering said antigen to a host animal whereby said animal generates an antibody response to said antigen, and exracting said antiserum from said animal.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-18 | 1984-05-19 | 1984-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-18 | 1988-05-19 | 1988-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-18 | 1992-05-19 | 1992-11-18 | $7700.00 | Window Closed / Lapsed |