US4016250A
Expired / Lapsed
US
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Method for testing for pregnancy
Assignee: Cornell Research Foundation Inc
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Filing: 1974-11-11
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Granted: 1977-04-05
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Expires: 1994-11-11
Abstract
Abstract Method for the determination of the hormone human chorionic gonadotropin (HCG), luteinizing hormone (LH), prolactin (PRL) and HCG-like material in an aqueous sample comprising contacting a highly specific receptor for these hormones with an aqueous sample to be tested, providing a means of indicating whether binding has taken place between the receptor and hormone possibly contained in the sample and observing the indicating means to determine the presence of the hormone in the sample. PRL may be determined separately or simultaneously with the HCG and LH. The receptor is a plasma membrane extract from the corpus luteum of an animal which possesses the receptor for HCG, LH, PRL and HCG-like material. Radioassay indicating means are preferred, according to which competitive protein binding between radioisotopically labeled hormone and hormone present in the sample is radiologically determined. The method has particular application to the determination to pregnancy in the human female.
Inventors
- 👤 Brij B. Saxena
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/834; Y10S530/85; Y10S530/851; Y10S530/852; Y10S530/853
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
94 words
In a method for the determination of human chorionic gonadotropin (HCG), luteinizing hormone (LH) or HCG-like material in an aqueous sample involving the steps of contacting said sample with an agent capable of selectively binding said hormone, providing a means for indicating whether said binding has taken place and observing said indicating means to determine the presence of said hormone in the sample, the improvement which comprises said agent capable of selectively binding said hormone being a plasma membrane extract from the corpus luteum of a species possessing the receptor for human chorionic gonadotropin.
Claim 2
Dependent Claim
94 words
In a method for the determination of human chorionic gonadotropin (HCG), luteinizing hormone (LH) or HCG-like material in an aqueous sample involving the steps of contacting said sample with an agent capable of selectively binding said hormone, providing a means for indicating whether said binding has taken place and observing said indicating means to determine the presence of said hormone in the sample, the improvement which comprises said agent capable of selectively binding said hormone being a plasma membrane extract from the corpus luteum of a species possessing the receptor for human chorionic gonadotropin.
Claim 3
Dependent Claim
51 words
The method as defined by claim 1, wherein said receptor is obtained by a method comprising the steps of preparing a finely, comminuted tissue homogenate from corpus luteum tissue, separating the plasma membranes from said homogenate and selecting the plasma membrane fraction capable of selectively binding biologically active human chorionic gonadotropin.
Claim 4
Dependent Claim
16 words
The method as defined by claim 1, wherein said receptor is derived from a pregnant female.
Claim 5
Dependent Claim
17 words
The method as defined by claim 3, wherein said female is in the first trimester of pregnancy.
Claim 6
Dependent Claim
33 words
The method as defined by claim 1, wherein said sample is a body fluid of a female mammal and whereby the results of said method provide a determination of the condition of pregnancy.
Claim 7
Dependent Claim
17 words
The method as defined by claim 5, wherein said body fluid is selected from blood or urine.
Claim 8
Dependent Claim
19 words
The method as defined by claim 1, wherein said receptor is obtained from the corpus luteum of a mammal.
Claim 9
Dependent Claim
26 words
The method as defined by claim 7, wherein said mammal is selected from the group consisting of a cow, a sheep, a pig, and a horse.
Claim 10
Dependent Claim
19 words
The method as defined by claim 8, wherein said receptor is obtained from the corpus luteum of a cow.
Claim 11
Dependent Claim
16 words
The method as defined by claim 1, wherein said indicating means comprises a radioassay measuring means.
Claim 12
Dependent Claim
18 words
The method as defined by claim 10, wherein said radioassay measuring means comprises radioisotopically labeled human chorionic gonadotropin.
Claim 13
Dependent Claim
93 words
The method as defined by claim 11, wherein said steps of providing and observing said indicating means comprise contacting said receptor and said sample in the presence of radioisotopically labeled human chorionic gonadotropin whereby part of said labeled hormone and part of said unlabeled hormone present in the sample are bound to said receptor, separating said receptor-bound hormones from unbound hormones in said aqueous sample and measuring the radioactivity of at least said separated receptor or said aqueous sample to determine the concentration of said hormone as a function of the measured radioactivity.
Claim 14
Dependent Claim
31 words
The method as defined by claim 12, wherein said radioisotopically labeled hormone is labeled with an isotope from the group consisting of 125 I, 131 I, 3 H and 14 C.
Claim 15
Dependent Claim
13 words
The method as defined by claim 13, wherein said isotope is 125 I.
Claim 16
Dependent Claim
37 words
The method as defined by claim 12, wherein said sample is a body fluid of a human female suspected of being pregnant and whereby the results of said method provide a determination of the condition of pregnancy.
Claim 17
Dependent Claim
18 words
The method as defined by claim 15, whereby said body fluid is selected from blood serum or urine.
Claim 18
Dependent Claim
14 words
The method as defined by claim 16, wherein said body fluid is blood serum.
Claim 19
Dependent Claim
20 words
The method as defined by claim 17, wherein said blood serum is utilized in an amount of about 0.1 ml.
Claim 20
Dependent Claim
51 words
A reagent for receptor-chemical determination of the hormone human chorionic gonadotropin, comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for the hormone human chorionic gonadotropin, said fraction being capable of selectively binding biologically active human chorionic gonadotropin.
Claim 21
Dependent Claim
51 words
A reagent for receptor-chemical determination of the hormone human chorionic gonadotropin, comprising in substantially pure form the specific fraction of plasma membrane extract from the corpus luteum of a species having the receptor for the hormone human chorionic gonadotropin, said fraction being capable of selectively binding biologically active human chorionic gonadotropin.
Claim 22
Independent Claim
63 words
A method for the preparation of a reagent for receptor-chemical determination of the hormone human chorionic gonadotropin, comprising the steps of preparing a finely comminuted tissue homogenate from corpus luteum tissue of a species having the receptor for human chorionic gonadotropin, separating the plasma membranes from said homogenate and selecting the plasma membrane fraction capable of selectively binding biologically active human chorionic goadotropin.
Claim 23
Independent Claim
63 words
A method for the preparation of a reagent for receptor-chemical determination of the hormone human chorionic gonadotropin, comprising the steps of preparing a finely comminuted tissue homogenate from corpus luteum tissue of a species having the receptor for human chorionic gonadotropin, separating the plasma membranes from said homogenate and selecting the plasma membrane fraction capable of selectively binding biologically active human chorionic goadotropin.
Claim 24
Independent Claim
70 words
A method for determining the hormone prolactin (PRL) in an aqueous sample, comprising contacting said sample with an agent capable of selectively binding said hormone, said agent being a plasma membrane extract from the corpus luteum of a species possessing the receptor for prolactin, providing a means for indicating whether said binding has taken place and observing said indicating means to determine the presence of said hormone in the sample.
Claim 25
Independent Claim
70 words
A method for determining the hormone prolactin (PRL) in an aqueous sample, comprising contacting said sample with an agent capable of selectively binding said hormone, said agent being a plasma membrane extract from the corpus luteum of a species possessing the receptor for prolactin, providing a means for indicating whether said binding has taken place and observing said indicating means to determine the presence of said hormone in the sample.
Claim 26
Dependent Claim
34 words
The method as defined by claim 21, wherein said receptor is obtained from the corpus luteum of a mammal selected from the group consisting of a cow, a sheep, a pig, and a horse.
Claim 27
Dependent Claim
49 words
The method as defined by claim 21, wherein said receptor is obtained by a method comprising the steps of preparing a finely communited tissue homogenate from corpus luteum tissue, separating the plasma membranes from said homogenate and selecting the plasma membrane fraction capable of selectively binding biologically active PRL.
Claim 28
Dependent Claim
22 words
The method as defined by claim 23, wherein said receptor is derived from a pregnant female in the first trimester of pregnancy.
Claim 29
Dependent Claim
16 words
The method as defined by claim 21, wherein said indicating means comprises a radioassay measuring means.
Claim 30
Dependent Claim
16 words
The method as defined by claim 25, wherein said radioassay measuring means comprises radioisotopically labeled prolactin.
Claim 31
Dependent Claim
91 words
The method as defined by claim 26, wherein said steps of providing and observing said indicating means comprise contacting said receptor and said sample in the presence of radioisotopically labeled prolactin whereby part of said labeled hormone and part of said unlabeled hormone present in the sample are bound to said receptor, separating said receptor-bound hormones from unbound hormones in said aqueous sample and measuring the radioactivity of at least said separated receptor or said aqueous sample to determine the concentration of said hormone as a function of the measured radioactivity.
Claim 32
Dependent Claim
31 words
The method as defined by claim 27, wherein said radioisotopically labeled hormone is labeled with an isotope from the group consisting of 125 I, 131 I, 3 H and 14 C.
Claim 33
Dependent Claim
13 words
The method as defined by claim 28, wherein said isotope is 125 I.
Claim 34
Dependent Claim
37 words
The method as defined by claim 27, wherein said sample is a body fluid of a human female suspected of being pregnant and whereby the results of said method provide a determination of the condition of pregnancy.
Claim 35
Dependent Claim
18 words
The method as defined by claim 30 wherein said body bluid is selected from blood serum or urine.
Claim 36
Dependent Claim
14 words
The method as defined by claim 31, wherein said body fluid is blood serum.
Claim 37
Independent Claim
94 words
A method for simultaneously determining a plurality of hormones in a single aqueous sample, comprising contacting said sample with an agent capable of selectively binding each of said hormones in the sample, said agent being a plasma membrane extract from a body organ of a species which possesses in said organ the specific receptor for each of said hormones, providing a separate, distinguishable means for each hormone to be determined for indicating whether said binding has taken place and observing each of said indicating means to determine the presence of each of said hormones.
Claim 38
Independent Claim
94 words
A method for simultaneously determining a plurality of hormones in a single aqueous sample, comprising contacting said sample with an agent capable of selectively binding each of said hormones in the sample, said agent being a plasma membrane extract from a body organ of a species which possesses in said organ the specific receptor for each of said hormones, providing a separate, distinguishable means for each hormone to be determined for indicating whether said binding has taken place and observing each of said indicating means to determine the presence of each of said hormones.
Claim 39
Dependent Claim
48 words
The method as defined by claim 33, wherein said hormones to be determined on said sample comprise human chorionic gonadotropin (HCG) and prolactin (PRL) and wherein said binding agent comprises a plasma membrane extract from the corpus luteum of a species possessing the receptor for HCG and PRL.
Claim 40
Dependent Claim
34 words
The method as defined by claim 34, wherein said receptor is obtained from the corpus luteum of an animal selected from the group consisting of a cow, a sheep, a pig and a horse.
Claim 41
Dependent Claim
51 words
The method as defined by claim 34, wherein said receptor is obtained by a method comprising the steps of preparing a finely comminuted tissue homogenate from corpus luteum tissue, separating the plasma membranes from said homogenate and selecting the plasma membrane fraction capable of selectively binding biologically active PRL and HCG.
Claim 42
Dependent Claim
21 words
The method as defined by claim 36, wherein said receptor is derived from a female in the first trimester of pregnancy.
Claim 43
Dependent Claim
16 words
The method as defined by claim 34, wherein said indicating means comprises a radioassay measuring means.
Claim 44
Dependent Claim
30 words
The method as defined by claim 38, wherein said measuring means comprises radioisotopically labeled HCG and a differently radiosotropically labeled PRL, the isotopes for labeling HCG and PRL being different.
Claim 45
Dependent Claim
107 words
The method as defined by claim 39, wherein said steps of providing and observing said indicating means comprise contacting said receptor and said sample in the presence of said radioisotopically labeled HCG and said radioisotopically labeled PRL, whereby a part of each of said labeled hormones and part of said unlabeled hormone present in the sample are bound to said receptor, separating said receptor-bound hormones from unbound hormones in said aqueous sample and measuring the radioactivity for each of said isotopes of at least said separated receptor or said aqueous sample to determine the concentration of each of said hormones as a function of the measured radioactivity.
Claim 46
Dependent Claim
22 words
The method as defined by claim 40, wherein said radioisotopically labeled HCG and PRL are labeled with two different isotopes of iodine.
Claim 47
Dependent Claim
16 words
The method as defined by claim 41, wherein said isotopes are 125 I and 131 I.
Claim 48
Dependent Claim
18 words
The method as defined by claim 42, wherein said sample is a body fluid of a human female.
Claim 49
Dependent Claim
15 words
The method as defined by claim 43, wherein said fluid is blood serum of urine.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-04 | 1980-10-04 | 1981-04-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-04 | 1984-10-04 | 1985-04-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-04 | 1988-10-04 | 1989-04-05 | $7700.00 | Window Closed / Lapsed |