Method for immobilizing enzymes

Claim 3

The method as defined in claim 1, wherein said adsorbent is selected from the group consisting of active terra alba, bentonite, kaolin, alumina, silica gel, molecular sieves and active carbon, and said monomer B is selected from the compound represented by the general formula: ##STR5## wherein R 1 is --CH 2 CH 2 --, R 2 is hydrogen, X is hydrogen or methyl, n is 2, 3 or 4, and m is 2, 3 or 4.

Claim 4

The method as defined in claim 2, wherein said adsorbent is selected from the group consisting of silica gel, kaolin and active carbon.

Claim 5

The method as defined in claim 3, wherein said B monomer is selected from the group consisting of 2-hydroxyethyl methacrylate and triethyleneglycol monomethacrylate.

Claim 6

The as defined in claim 1, wherein said B monomer contains a second monomer C having water-miscibility different from that of said Group B monomer in amount up to 30% of the total weight of the monomers.

Claim 7

The method as defined in claim 5, wherein said adsorbent is selected from the group consisting of active terra alba, bentonite, kaolin, alumina, silica gel, molecular sieves, and active carbon and said water-miscible monomer is selected from the compounds represented by the general formula: ##STR6## wherein R 1 is --CH 2 CH 2 --, R 2 is hydrogen, X is hydrogen or methyl, n is 2, 3 or 4, and m is 2, 3 or 4, and said second monomer is a polyfunctional partly water-miscible monomer.

Claim 8

The method as defined in claim 6, wherein said adsorbent is selected from the group consisting of silica gel, kaolin, and active carbon.

Claim 9

The method as defined in claim 7, wherein said monomer B is selected from the group consisting of 2-hydroxyethyl methacrylate and triethyleneglycol monomethacrylate, and the second monomer is selected from the group consisting of methylolacrylamide, diallyl itaconate, ethyleneglycol dimethacrylate, hexanediol diacrylate, vinylpyrrolidone, trimethylolpropane trimethacrylate.

Claim 10

A porous three dimensional reticular structure comprising adsorbed enzyme or enzyme-containing cells whenever obtained by (a) adsorbing said enzymes or enzyme-containing cells on an adsorbent selected from the group consisting of inorganic natural earth adsorbents, synthetic inorganic adsorbents and active carbon in an aqueous medium, (b) admixing the aqueous dispersion of said adsorbent on which enzymes or enzyme-containing cells have been adsorbed with a polymerizable monomer selected from the groups consisting of compounds represented by the general formula ##STR7## wherein R 1 is a group --CH 2 CH 2 -- or --CH(CH 3 )CH 2 --, R 2 is hydrogen or methyl or a group represented by ##STR8## X is hydrogen or methyl, n is an integer 2, 3 or 4 and m is an integer from 2 to 20 and (c) effecting polymerization at a temperature between -20° C. and -80° C., to form a porous gel lump in which enzymes or enzyme-containing cells are distributed adsorbed on inorganic adsorbent, and through which a substrate solution can freely pass.

Claim 11

A porous three dimensional reticular structure comprising adsorbed enzyme or enzyme-containing cells whenever obtained by (a) adsorbing said enzymes or enzyme-containing cells on an adsorbent selected from the group consisting of inorganic natural earth adsorbents, synthetic inorganic adsorbents and active carbon in an aqueous medium, (b) admixing the aqueous dispersion of said adsorbent on which enzymes or enzyme-containing cells have been adsorbed with a polymerizable monomer selected from the groups consisting of compounds represented by the general formula ##STR7## wherein R 1 is a group --CH 2 CH 2 -- or --CH(CH 3 )CH 2 --, R 2 is hydrogen or methyl or a group represented by ##STR8## X is hydrogen or methyl, n is an integer 2, 3 or 4 and m is an integer from 2 to 20 and (c) effecting polymerization at a temperature between -20° C. and -80° C., to form a porous gel lump in which enzymes or enzyme-containing cells are distributed adsorbed on inorganic adsorbent, and through which a substrate solution can freely pass.