Patent US4196265A successfully refreshed from Google Patents.
US4196265A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing antibodies
Assignee: The Wistar Institute
•
Filing: 1978-08-11
•
Granted: 1980-04-01
•
Expires: 1998-08-11
Abstract
Abstract Continuous cell lines of genetically stable fused cell hybrids capable of producing large amounts of monoclonal antibodies against specific viruses and their antigenic determinants have been developed. The cell lines are fused cell hybrids between viral antibody producing cells and myeloma cells. Fused cell hybrids between influenza virus-primed mouse spleen cells and mouse myeloma cells can be maintained indefinitely in culture and continue to produce large amounts of anti-influenza antibody.
Inventors
- 👤 Hilary Koprowski
- 👤 Walter U. Gerhard
- 👤 Carlo M. Croce
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:01
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/08
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
31 words
A process for producing viral antibodies comprising fusing a viral antibody producing cell and a myeloma cell to provide a fused cell hybrid, culturing said hybrid and collecting the viral antibodies.
Claim 2
Dependent Claim
31 words
A process for producing viral antibodies comprising fusing a viral antibody producing cell and a myeloma cell to provide a fused cell hybrid, culturing said hybrid and collecting the viral antibodies.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein said hybrid is cultured in vitro.
Claim 4
Dependent Claim
17 words
The process of claim 2 wherein said hybrid is cloned and cultured in a medium containing hypoxanthine-aminopterin-thymidine.
Claim 5
Dependent Claim
18 words
The process of claim 1 wherein said hybrid is injected into a histocompatible animal and cultured in vivo.
Claim 6
Dependent Claim
26 words
The process of claim 1 wherein said viral antibody producing cell is a cell selected from the group consisting of spleen cells and lymph node cells.
Claim 7
Dependent Claim
17 words
The process of claim 5 wherein said spleen is mouse spleen and said myeloma is mouse myeloma.
Claim 8
Dependent Claim
13 words
The process of claim 5 wherein the said mouse is a BALB/c mouse.
Claim 9
Dependent Claim
17 words
The process of claim 6 wherein said myeloma is (P3 63 Ag8) derived from the MOPC-21 line.
Claim 10
Dependent Claim
20 words
The process of claim 1 wherein said viral antibody producing cell is obtained from an animal immunized with a virus.
Claim 11
Dependent Claim
20 words
The process of claim 1 wherein said viral antibody producing cell is obtained from a mouse immunized with a virus.
Claim 12
Dependent Claim
21 words
The process of claim 1 wherein said viral antibody producing cell is obtained from an aminal immunized with an influneza virus.
Claim 13
Dependent Claim
21 words
The process of claim 1 wherein said viral antibody producing cell is obtained from an animal immunized with a rabies virus.
Claim 14
Dependent Claim
62 words
A process for producing antibodies comprising injecting a BALB/c mouse with a virus to induce antibody formation of spleen cells of said mouse, forming a fused cell hybrid of said viral antibody producing splenic cells with a BALB/c myeloma cell clone (P3×63 Ag8) derived from the MOPC-21 line, culturing said hybrid in vitro in selective HAT medium and harvesting the antibodies produced.
Claim 15
Dependent Claim
62 words
A process for producing antibodies comprising injecting a BALB/c mouse with a virus to induce antibody formation of spleen cells of said mouse, forming a fused cell hybrid of said viral antibody producing splenic cells with a BALB/c myeloma cell clone (P3×63 Ag8) derived from the MOPC-21 line, culturing said hybrid in vitro in selective HAT medium and harvesting the antibodies produced.
Claim 16
Dependent Claim
12 words
The process of claim 13 wherein said virus is an influenza virus.
Claim 17
Dependent Claim
12 words
The process of claim 13 wherin said virus is simian virus 40.
Claim 18
Dependent Claim
12 words
The process of claim 13 wherein said virus is a rabies virus.
Claim 19
Dependent Claim
36 words
A composition comprising a continuous cell line which produces influenza antibodies in vitro in hypoxanthine-aminopterin-thymidine medium comprising a fused cell hybrid of influenza primed BALB/c mouse spleen and MOPC-21 mouse myeloma and a culture medium therefor.
Claim 20
Dependent Claim
36 words
A composition comprising a continuous cell line which produces influenza antibodies in vitro in hypoxanthine-aminopterin-thymidine medium comprising a fused cell hybrid of influenza primed BALB/c mouse spleen and MOPC-21 mouse myeloma and a culture medium therefor.
Claim 21
Dependent Claim
14 words
The composition of claim 17 wherein said continuous cell consists essentially of clone HK-PEG-1.
References Cited (Backward Citations)
US2768114A
Cited
GB1300391A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-01 | 1983-10-01 | 1984-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-01 | 1987-10-01 | 1988-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-01 | 1991-10-01 | 1992-04-01 | $7700.00 | Window Closed / Lapsed |