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US4177107A
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Process for producing composition containing insolubilized enzyme and/or insolubilized bacterial cells
Assignee: Japan Atomic Energy Agency
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Filing: 1977-01-28
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Granted: 1979-12-04
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Expires: 1997-01-28
Abstract
Abstract A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer.
Inventors
- 👤 Minoru Kumakura
- 👤 Masaru Yoshida
- 👤 Isao Kaetsu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:47
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
214 words
A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer selected from the group consisting of a compound having the formula: ##STR10## wherein X is H or methyl, R 1 is --CH 2 -- n wherein n is an integer of 1 to 6, ##STR11## CH 2 --CH 2 --O-- m wherein m is an integer of 2 or more, or ##STR12## wherein l is an integer of 2 or more, and when R 1 is --CH 2 -- n or ##STR13## R 2 is --OH, --OCH 3 or ##STR14## wherein X is as defined above, when R 1 is --CH 2 --CH 2 --O m or ##STR15## R 2 is hydrogen, methyl, or ##STR16## wherein X is as defined above; or a compound having the formula: ##STR17## wherein X is as defined above; a compound having the formula ##STR18## wherein R is ethyl, n-propyl or isopropyl and X is as defined above; and mixtures thereof, and (ii) the step of irradiating the mixtures by means of an ionizing radiation at a temperature of less than -10° C.
Claim 2
Dependent Claim
214 words
A process for producing a composition containing insolubilized enzyme and/or insolubilized bacterial cells which comprises (i) the step of mixing (a) an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells, (b) at least one porous adsorbent and (c) at least one vitrifiable monomer selected from the group consisting of a compound having the formula: ##STR10## wherein X is H or methyl, R 1 is --CH 2 -- n wherein n is an integer of 1 to 6, ##STR11## CH 2 --CH 2 --O-- m wherein m is an integer of 2 or more, or ##STR12## wherein l is an integer of 2 or more, and when R 1 is --CH 2 -- n or ##STR13## R 2 is --OH, --OCH 3 or ##STR14## wherein X is as defined above, when R 1 is --CH 2 --CH 2 --O m or ##STR15## R 2 is hydrogen, methyl, or ##STR16## wherein X is as defined above; or a compound having the formula: ##STR17## wherein X is as defined above; a compound having the formula ##STR18## wherein R is ethyl, n-propyl or isopropyl and X is as defined above; and mixtures thereof, and (ii) the step of irradiating the mixtures by means of an ionizing radiation at a temperature of less than -10° C.
Claim 3
Dependent Claim
26 words
The process as defined in claim 1, wherein the irradiation is carried out at a temperature in the range of from -80° C. to -25° C.
Claim 4
Dependent Claim
59 words
The process as defined in claim 1, wherein the monomer is selected from the group consisting of hydroxyethyl acrylate, hydroxypropyl methacrylate, glycidylacrylate, glycidylmethacrylate, tetraethyleneglycol methacrylate, tetraethyleneglycol acrylate, diethyleneglycol dimethacrylate, diethyleneglycol acrylate, polyethyleneglycol dimethacrylate, polyethyleneglycol diacrylate, neopentylglycol dimethacrylate, neopentylglycol diacrylate, trimethylol propane trimethacrylate, trimethylolpropane triacrylate, butanediol dimethacrylate, butanediol diacrylate, hexanediol dimethacrylate, hexanediol diacrylate, ethyleneglycol dimethacrylate, ethyleneglycol diacrylate and mixtures thereof.
Claim 5
Dependent Claim
34 words
The process as defined in claim 1, wherein the adsorbent is selected from the group consisting of molecular sieves, activated carbon, Kanuma soil, porous argil, porous ion-exchange resin, particulate calcium sulfate and mixtures thereof.
References Cited (Backward Citations)
US3577512A
Cited
US3860490A
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US3962038A
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US3871964A
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US3859169A
Cited
US3933587A
Cited
US4025391A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-03 | 1983-06-04 | 1983-12-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-03 | 1987-06-04 | 1987-12-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-03 | 1991-06-04 | 1991-12-04 | $7700.00 | Window Closed / Lapsed |