US4168300A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of removal of hepatitis virus
Assignee: Phadia AB
•
Filing: 1976-07-06
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Granted: 1979-09-18
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Expires: 1996-07-06
Abstract
Abstract Hepatitis virus is removed from a biological material contaminated with the virus by contact with a preparation. The preparation may be agarose gel or beaded polyacrylamide plastic coupled with a variety of hydrophobic ligands.
Inventors
- 👤 Lars-Olov Andersson
- 👤 Hakan G. Borg
- 👤 Gudrun M. Einarsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:15
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61L; A61L2/00; A61L2/02; A61L2/16; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; A61L2103/00; A61L2103/05; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10151; Y; Y10; Y10S; Y10S436/00; Y10S436/82; Y10S436/825; Y10S530/00; Y10S530/806; Y10S530/82; Y10S530/826; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
177 words
A method for removing hepatitis virus from a biological material contaminated with said virus, comprising the step of bringing said hepatitis virus contaminated material in contact with a preparation which is composed of a water-insoluble, water-permeable, cross-linked gel-matrix substance selected from (a) a high molecular weight water-insoluble, water-permeable adsorbent, gel-matrix-forming carbohydrate of at least 1,000 molecular weight inert to ethanol, dioxane, dimethylformamide, sodium hydroxide at pH up to about 8, 0.5 M Na 2 CO 3 solution, acetic acid at pH 4, and 1 M NaCl, and (b) a beaded polyacrylamide plastic; and onto which gel-matrix substance, directly or through a divalent molecule extender group selected from cysteamine, propylene, and an alkylene diamine having from 2 to about 6 carbons in the alkylene chain, there is coupled a hydrophobic ligand selected from the alkylamino groups from octylamino to octadecylamino, and the dodecyl-2-amino and the caprylhydrazido groups through the amino nitrogen of the alkylamino group or the caprylhydrazido group respectively, heptanoic acid, an alkyl succinic acid from octylsuccinic acid to dodecylsuccinic acid, naphthylacetic acid, and cholesterol hydrogen succinate.
Claim 2
Independent Claim
177 words
A method for removing hepatitis virus from a biological material contaminated with said virus, comprising the step of bringing said hepatitis virus contaminated material in contact with a preparation which is composed of a water-insoluble, water-permeable, cross-linked gel-matrix substance selected from (a) a high molecular weight water-insoluble, water-permeable adsorbent, gel-matrix-forming carbohydrate of at least 1,000 molecular weight inert to ethanol, dioxane, dimethylformamide, sodium hydroxide at pH up to about 8, 0.5 M Na 2 CO 3 solution, acetic acid at pH 4, and 1 M NaCl, and (b) a beaded polyacrylamide plastic; and onto which gel-matrix substance, directly or through a divalent molecule extender group selected from cysteamine, propylene, and an alkylene diamine having from 2 to about 6 carbons in the alkylene chain, there is coupled a hydrophobic ligand selected from the alkylamino groups from octylamino to octadecylamino, and the dodecyl-2-amino and the caprylhydrazido groups through the amino nitrogen of the alkylamino group or the caprylhydrazido group respectively, heptanoic acid, an alkyl succinic acid from octylsuccinic acid to dodecylsuccinic acid, naphthylacetic acid, and cholesterol hydrogen succinate.
Claim 3
Dependent Claim
20 words
The method according to claim 1, wherein the water-permeable matrix substance is a beaded agarose gel or a polyacrylamide plastic.
Claim 4
Dependent Claim
12 words
The method according to claim 2, wherein said matrix substance is polyacrylamide.
Claim 5
Dependent Claim
17 words
The method according to claim 3, wherein said polyacrylamide is a conjugate with propylene-decylamine as the ligand.
Claim 6
Dependent Claim
15 words
The method according to claim 2, wherein the matrix substance is the beaded agarose gel.
Claim 7
Dependent Claim
27 words
The method as claimed in claim 5, wherein the ligand coupled to said gel-matrix is selected from the octylamino, decylamino, dodecylamino, tetradecylamino, octadecylamino, dodecylamino-2, and caprylhydrazido groups.
Claim 8
Dependent Claim
55 words
The method as claimed in claim 5, wherein the ligand coupled to said gel-matrix directly or with its intermediary linked extender group is selected from ethylenediamine-octyl succinic acid, butylenediamine-octylsuccinic acid, hexamethylenediamine-octylsuccinic acid, octylamine, decylamine, dodecylamine, octadecylamine, hexamethylenediamine-dodecyl succinic acid, caprylhydrazide, tetradecylamine, 2-amino-dodecane, ethylenediamine-undeceene-10 acid, cysteamine-octyl succinic acid, hexamethylenediamine-naphthyl-1-acetic acid, hexamethylenediamine-cholesterol hydrogen succinate, and hexamethylenediamine-cholic acid.
Claim 9
Dependent Claim
18 words
The method according to claim 7, wherein said agarose gel is a conjugate with caprylhydrazide as the ligand.
Claim 10
Dependent Claim
18 words
The method according to claim 7, wherein said agarose gel is a conjugate with tetradecylamine as the ligand.
Claim 11
Dependent Claim
18 words
The method according to claim 7, wherein said agarose gel is a conjugate with 2-amino-dodecane as the ligand.
Claim 12
Dependent Claim
25 words
The method according to claim 7, wherein the hydrophobic ligand is coupled to the gel-matrix substance through the intervention of a divalent molecule extender group.
Claim 13
Dependent Claim
16 words
The method according to claim 11, wherein said agarose gel is a conjugate with ethylenediamine-octylsuccinic acid.
Claim 14
Dependent Claim
16 words
The method according to claim 11, wherein said agarose gel is a conjugate with hexamethylenediamine-dodecylsuccinic acid.
Claim 15
Dependent Claim
15 words
The method according to claim 11, wherein said agarose gel is a conjugate with ethylenediamine-undeceene-10-acid.
Claim 16
Dependent Claim
16 words
The method according to claim 11, wherein said agarose gel is a conjugate with cyteamine-octylsuccinic acid.
Claim 17
Dependent Claim
16 words
The method according to claim 11, wherein said agarose gel is a conjugate with hexamethylenediamine-naphthyl-1-acetic acid.
Claim 18
Dependent Claim
17 words
The method according to claim 11, wherein said agarose gel is a conjugate with hexamethylenediamine-cholesterol hydrogen succinate.
Claim 19
Dependent Claim
16 words
The method according to claim 11, wherein said agarose gel is a conjugate with hexamethylenediamine-cholic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-17 | 1983-03-19 | 1983-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-17 | 1987-03-19 | 1987-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-17 | 1991-03-19 | 1991-09-18 | $7700.00 | Window Closed / Lapsed |