US4168205A
Expired / Lapsed
US
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Method for the determination of substrates or enzyme activities
Assignee: Roche Diagnostics GmbH
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Filing: 1977-04-28
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Granted: 1979-09-18
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Expires: 1997-04-28
Abstract
Abstract In a process for the determination of substrates or enzyme activities in a test batch, utilizing a conventional redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase; the addition of ascorbate oxidase substantially eliminates the interfering effect of ascorbic acid frequently found in the test sample.
Inventors
- 👤 Josef Danninger
- 👤 Ulfert Deneke
- 👤 Gunter Lang
- 👤 Gerhard Michal
- 👤 Peter Roeschlau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:20
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
43 words
In a process for the determination of substrates or enzyme activities in a test batch, utilizing a redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase obtained from small marrow (Curcurbita pepo medullosa).
Claim 2
Dependent Claim
43 words
In a process for the determination of substrates or enzyme activities in a test batch, utilizing a redox reaction as a measurement reaction, the improvement comprising carrying out the process in the presence of ascorbate oxidase obtained from small marrow (Curcurbita pepo medullosa).
Claim 3
Dependent Claim
17 words
Process as claimed in claim 1 wherein 0.002 to 100 U ascorbate oxidase/ml. test batch are used.
Claim 4
Dependent Claim
17 words
Process as claimed in claim 2 wherein 0.01 to 30 U ascorbate oxidase/ml. test batch are used.
Claim 5
Dependent Claim
19 words
Process as claimed in claim 1 wherein the ascorbate oxidase is used together with an enzyme forming hydrogen peroxide.
Claim 6
Dependent Claim
17 words
Process as claimed in claim 1 wherein the ascorbate oxidase is used together with a tetrazolium salt.
Claim 7
Dependent Claim
25 words
Process as claimed in claim 1 wherein the ascorbate oxidase is used together with a phenol capable of being coupled oxidatively with a nucleophilic reagent.
Claim 8
Dependent Claim
20 words
Process as claimed in claim 1 wherein it is carried out at a pH value of from 4.0 to 8.5.
Claim 9
Dependent Claim
20 words
Process as claimed in claim 7 wherein there is used an ascorbate oxidase obtained from small marrows (Curcurbita pepo medullosa).
Claim 10
Dependent Claim
41 words
Reagent for the enzymatic determination of substrates or enzyme activities, comprising a system for the determination of a substrate or enzyme with a redox reaction as measurement reaction, wherein ascorbate oxidase obtained from small marrow (Curcurbita pepo medullosa) is additionally present.
Claim 11
Dependent Claim
41 words
Reagent for the enzymatic determination of substrates or enzyme activities, comprising a system for the determination of a substrate or enzyme with a redox reaction as measurement reaction, wherein ascorbate oxidase obtained from small marrow (Curcurbita pepo medullosa) is additionally present.
Claim 12
Dependent Claim
31 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of glucose and comprises peroxidase, glucose oxidase, o-dianisidine or 2-azino-di-3-ethyl-benzthiazoline-6-sulphonate and buffer.
Claim 13
Dependent Claim
31 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of uric acid and comprises peroxidase, uricase, a phenol, aminoantipyrine and buffer.
Claim 14
Dependent Claim
11 words
Reagent as claimed in claim 11 wherein the phenol is 2,4-dichlorophenol.
Claim 15
Dependent Claim
34 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of glucose and comprises hexokinase, glucose-6-phosphate dehydrogenase, nicotinamide-adenine-dinucleotide phosphate, diaphorase, a tetrazolium salt and buffer.
Claim 16
Dependent Claim
32 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of glutamate and comprises glutamate dehydrogenase, diaphorase, nicotinamide-adenine-dinucleotide, a tetrazolium salt and buffer.
Claim 17
Dependent Claim
28 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of tyrosine and comprises tyrosinase, 3-methyl-6-potassium sulphonyl-benzthiazolone-2-hydrazone and buffer.
Claim 18
Dependent Claim
29 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate serves for the determination of pyrocatechol and comprises diphenol oxidase, 3-methyl-6-potassium sulphonyl-benzthiazolone-2-hydrazone and buffer.
Claim 19
Dependent Claim
26 words
Reagent as claimed in claim 9 wherein the system for the determination of a substrate and the ascorbate oxidase are impregnated with an absorbent carrier material.
Claim 20
Dependent Claim
22 words
Reagent as claimed in claim 17 comprising an absorbent carrier impregnated with glucose oxidase, peroxidase, ascorbate oxidase, a 9-(γ-dimethylaminopropyl)-6-chloro-3-aminocarbazole salt and buffer.
Claim 21
Dependent Claim
29 words
Reagent as claimed in claim 18 comprising an absorbent carrier impregnated with an ethylenediamine-tetraacetic acid salt, dioctyl sodium sulphosuccinate, 2,5-dimethylhexane-2,5-dihydroperoxide, phosphoric acid trimorpholide, ascorbate oxidase, 3,3',5,5'-tetramethylbenzidine, phenanthridine and buffer.
Claim 22
Dependent Claim
13 words
Reagent as claimed in claim 17 wherein the aborbent carrier is a paper.
Claim 23
Dependent Claim
52 words
Reagent as claimed in claim 9 wherein the ascorbate oxidase is impregnated into a water-soluble sheet of carrier material and the system for the determination of a substrate is impregnated into a water-insoluble sheet of absorbent carrier material, the water-soluble and the water-insoluble carrier materials being placed on top of one another.
Claim 24
Dependent Claim
13 words
Reagent as claimed in claim 17 comprising ascorbate oxidase, gum guaiac and buffer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-17 | 1983-03-19 | 1983-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-17 | 1987-03-19 | 1987-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-17 | 1991-03-19 | 1991-09-18 | $7700.00 | Window Closed / Lapsed |