US3721607A
Expired / Lapsed
US
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Reagent composition and process for the determination of glucose
Assignee: Roche Diagnostics GmbH
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Filing: 1971-08-11
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Granted: 1973-03-20
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Expires: 1991-08-11
Abstract
Abstract Compositions comprising glucose oxidase, peroxidase, a chromogen, a buffer, an azide, and 2,2''-azino-di-(3ethylbenzothiazoline-6-sulfonic acid) provide remarkably stable test reagents for the enzymatic determination of glucose.
Inventors
- 👤 W Gruber
- 👤 E Bernt
- 👤 K Gawehn
- 👤 H Bergmeyer
- 👤 W Werner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/30
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
15 words
Composition as claimed in claim 1 wherein the 2,2''-azino-di-(3-ethyl-benzothiazoline-6-sulfonic acid) acts as the said chromogen.
Claim 2
Dependent Claim
14 words
Composition as claimed in claim 1 wherein the azide is an alkali metal azide.
Claim 3
Dependent Claim
12 words
Composition as claimed in claim 1 wherein said buffer is phosphate buffer.
Claim 4
Dependent Claim
52 words
Composition as claimed in claim 1 which comprises: a buffer, pH 6.2 to 7.5 2.5 to 15 X 103 U glucose oxidase, 30 to 1000 U peroxide, 0.05 to 0.2 grams of an azIde, and 0.25 to 1.5 grams 2,2''-azino-di-(3-ethyl-benzothiazoline-6-sulfonic acid), based on each liter of ultimate applied aqueous reagent composition solution.
Claim 5
Dependent Claim
59 words
Composition as claimed in claim 1 which comprises: a buffer, pH 6.2 to 7.5 2.5 to 15 X 103 U glucose oxidase, 30 to 1000 U peroxidase, 0.05 to 0.2 grams of an azide, 1 to 10 milligrams of 2,2''-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid), and 50 to 70 milligrams of o-dianisidine, based on each liter of ultimate applied aqueous reagent composition solution.
Claim 6
Dependent Claim
13 words
Composition as claimed in claim 5 wherein said composition is in solid form.
Claim 7
Dependent Claim
17 words
Composition as claimed in claim 5 wherein said composition is in the form of an aqueous solution.
Claim 8
Dependent Claim
49 words
Composition as claimed in claim 1 which comprises: 0.1M phosphate buffer, pH 6.5 to 7.2, 9 to 12 X 103 U glucose oxidase, 150 to 500 U peroxidase, 0.1 gram of an azide, and 0.5 gram 2,2''-azino-di-(3-ethyl-benzothiazoline-6-sulfonic acid), based on each liter of ultimate applied aqueous reagent composition solid.
Claim 9
Dependent Claim
56 words
Composition as claimed in claim 1 which comprises: 0.1M phosphate buffer, pH 6.5 to 7.2, 9 to 12 X 103 U glucose oxidase, 150 to 500 U peroxidase, 0.1 gram of an azide, 2 to 5 milligrams 2,2''-azino-di-(3-ethyl-benzothiazoline-6-sulfonic acid), and 50 to 70 milligrams o-dianisidine, based on each liter of ultimate applied aqueous reagent composition solution.
Claim 10
Dependent Claim
12 words
Composition as claimed in claim 9 wherein said azide is sodium azide.
Claim 11
Dependent Claim
31 words
Method for the enzymatic determination of glucose which method comprises contacting a test sample with a reagent composition comprising glucose oxidase, peroxidase, a chromogen, a buffer, an azide, and 2,2''-azino-di-(3-ethyl-benzothiazoline-6-sulfonic acid).
Claim 12
Dependent Claim
28 words
Method as claimed in claim 12 wherein the intensity of the colored material formed is measured and the glucose concentration present in the test sample is determined therefrom.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-03-19 | 1976-09-18 | 1977-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-03-19 | 1980-09-18 | 1981-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-03-19 | 1984-09-18 | 1985-03-20 | $7700.00 | Window Closed / Lapsed |