US4143124A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antigen-antibody analysis with solid phase rf and c1q
Assignee: Bayer Corp
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Filing: 1975-05-19
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Granted: 1979-03-06
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Expires: 1995-05-19
Abstract
Abstract Biological fluids, such as serum, are analyzed for the presence, nature and/or amount of antibodies, antigens and antibody:antigen complexes therein using as a reagent, insolubilized rheumatoid factor or insolubilized C1q. These reagents are themselves novel. They bind to antibody: antigen complexes, but not to free antibodies or antigens, and complexes can thus be removed from mixtures thereof with other materials, such as antibodies and antigens.
Inventors
- 👤 Pierre L. Masson
- 👤 Joseph F. Heremans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54306; G01N33/54313; G01N33/564; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S436/00; Y10S436/811; Y10S436/819; Y10S436/821; Y10S436/823; Y10S436/826
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
117 words
A method for quantitatively analyzing an aqueous biological fluid sample for an antibody-antigen complex therein, comprising: (a) forming a mixture of the sample with a substance selected from the group consisting of rheumatoid factor and complement component, said substance being convalently bonded to particles of a naturally-occurring solid phase substrate which is insoluble in aqueous fluids; (b) allowing the substance on the particles selectively to react with antibody-antigen complex present in the mixture thereby selectively to bind said complex to said particles; and (c) assaying the particles or the remainder of the mixture to determine the amount of antibody-antigen complex bound thereto or remaining therein and thereby determining the amount of antibody-antigen complex present in the sample.
Claim 2
Independent Claim
117 words
A method for quantitatively analyzing an aqueous biological fluid sample for an antibody-antigen complex therein, comprising: (a) forming a mixture of the sample with a substance selected from the group consisting of rheumatoid factor and complement component, said substance being convalently bonded to particles of a naturally-occurring solid phase substrate which is insoluble in aqueous fluids; (b) allowing the substance on the particles selectively to react with antibody-antigen complex present in the mixture thereby selectively to bind said complex to said particles; and (c) assaying the particles or the remainder of the mixture to determine the amount of antibody-antigen complex bound thereto or remaining therein and thereby determining the amount of antibody-antigen complex present in the sample.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein the solid phase substrate is aggregated immunoglobulins.
Claim 4
Independent Claim
135 words
A method for quantitatively analyzing an aqueous biological fluid sample for antibody or antigen therein, comprising: (a) adding to the sample the appropriate antigen or antibody, respectively, to form antibody-antigen complex; (b) forming a mixture of the sample with a substance selected from the group consisting of rheumatoid factor and complement component C1q, the substance being covalently bonded to particles of a naturally occurring solid phase substrate which is insoluble in aqueous fluids; (c) allowing the substance in the particles selectively to react with antibody-antigen complex present in the mixture thereby selectively to bind the complex to the particles; and (d) assaying the particles or the remainder of the mixture to determine the amount of antibody-antigen complex bound thereto or remaining therein and thereby determining the amount of antibody or antigen present in the sample.
Claim 5
Independent Claim
135 words
A method for quantitatively analyzing an aqueous biological fluid sample for antibody or antigen therein, comprising: (a) adding to the sample the appropriate antigen or antibody, respectively, to form antibody-antigen complex; (b) forming a mixture of the sample with a substance selected from the group consisting of rheumatoid factor and complement component C1q, the substance being covalently bonded to particles of a naturally occurring solid phase substrate which is insoluble in aqueous fluids; (c) allowing the substance in the particles selectively to react with antibody-antigen complex present in the mixture thereby selectively to bind the complex to the particles; and (d) assaying the particles or the remainder of the mixture to determine the amount of antibody-antigen complex bound thereto or remaining therein and thereby determining the amount of antibody or antigen present in the sample.
Claim 6
Dependent Claim
14 words
A method according to claim 3 wherein the solid phase substrate is aggregated immunoglobulins.
References Cited (Backward Citations)
US3639559A
Cited
US3639558A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-03-05 | 1982-09-04 | 1983-03-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-03-05 | 1986-09-04 | 1987-03-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-03-05 | 1990-09-04 | 1991-03-06 | $7700.00 | Window Closed / Lapsed |