US4090919A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Water-insoluble tannin preparation for immobilization of proteins
Assignee: Tanabe Pharma Corp
•
Filing: 1977-01-19
•
Granted: 1978-05-23
•
Expires: 1997-01-19
Abstract
Abstract A water-insoluble tannin preparation is obtained by covalent binding or physical adsorption of tannin onto a water-insoluble, hydrophilic carrier. The preparation has a specific affinity for proteins and can be used as an adsorbent for purification, isolation and/or separation of proteins (e.g., Enzymes, albumin, globulin, hormonal proteins) from a mixture of compounds. Further, the water-insoluble tannin preparation having a catalytically active enzyme absorbed thereon can be used as a heterogeneous catalyst to induce enzymatic reactions.
Inventors
- 👤 Ichiro Chibata
- 👤 Tetsuya Tosa
- 👤 Takao Mori
- 👤 Taizo Watanabe
- 👤 Ryujiro Sano
- 👤 Yuhsi Matuo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:13
CPC Classifications
C; C07; C07H; C07H13/00; C07H13/12; B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3212; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; C07H15/00; C07H15/02; C07H15/04; C07H15/08; C12; C12N; C12N11/00; C12N11/02; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/814; Y10S435/815; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 63
Claim 1
Independent Claim
46 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane.
Claim 2
Dependent Claim
46 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane.
Claim 3
Dependent Claim
31 words
The tannin preparation of claim 1 wherein said cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane are employed in combination with at least one of alkylenediamine, aminoalkanol, aminoalkanoic acid, alkylenediol and haloalkanoyl halide.
Claim 4
Dependent Claim
135 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n --NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6.
Claim 5
Dependent Claim
135 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n --NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6.
Claim 6
Dependent Claim
133 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a polysaccharide through a linkage selected from the group consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR12## and ##STR13## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6.
Claim 7
Dependent Claim
133 words
A water-insoluble tannin preparation consisting essentially of tannin covalently bound to a polysaccharide through a linkage selected from the group consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR12## and ##STR13## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6.
Claim 8
Dependent Claim
15 words
The tannin preparation of claim 4 wherein said linkage is --CH 2 CH(OH)CH 2 --.
Claim 9
Dependent Claim
19 words
The tannin preparation of claim 4 wherein said linkage is --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --.
Claim 10
Dependent Claim
19 words
The tannin preparation of claim 4 wherein said linkage is --CH 2 CH(OH)CH 2 NH(CH 2 ) n --Y--.
Claim 11
Dependent Claim
15 words
The tannin preparation of claim 4 wherein said linkage is --CONH(CH 2 ) n --Y--.
Claim 12
Dependent Claim
19 words
The tannin preparation of claim 4 wherein said linkage is --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--.
Claim 13
Dependent Claim
19 words
The tannin preparation of claim 4 wherein said linkage is --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--.
Claim 14
Dependent Claim
11 words
The tannin preparation of claim 4 wherein said linkage is ##STR14##
Claim 15
Dependent Claim
11 words
The tannin preparation of claim 4 wherein said linkage is ##STR15##
Claim 16
Dependent Claim
11 words
The tannin preparation of claim 4 wherein said linkage is ##STR16##
Claim 17
Dependent Claim
37 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide-O-CH 2 CH(OH)CH 2 O(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 18
Dependent Claim
37 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide-O-CH 2 CH(OH)CH 2 O(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 19
Dependent Claim
37 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 20
Dependent Claim
37 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 21
Dependent Claim
33 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide--O--CONH(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 22
Dependent Claim
33 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an alkyl-polysaccharide represented by the formula: Polysaccharide--O--CONH(CH 2 ) m H wherein m is an integer of 1 to 16.
Claim 23
Dependent Claim
20 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to a phenoxyalkyl-polysaccharide represented by the formula: ##STR17##
Claim 24
Dependent Claim
20 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to a phenoxyalkyl-polysaccharide represented by the formula: ##STR17##
Claim 25
Dependent Claim
38 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an aminoalkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16.
Claim 26
Dependent Claim
38 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an aminoalkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16.
Claim 27
Dependent Claim
27 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an aminoalkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2
Claim 28
Dependent Claim
27 words
A water-insoluble tannin preparation consisting essentially of tannin bound by physical adsorption to an aminoalkyl-polysaccharide represented by the formula: Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2
Claim 29
Independent Claim
73 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer and an amino-polymer, which comprises reacting tannin or said hydroxy- or amino-polymer with cyanogen halide, epihalohydrin, α,ω-bis( 2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin or polymer and reacting said activated tannin with said hydroxy- or amino-polymer; or reacting said activated polymer with tannin.
Claim 30
Independent Claim
73 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer and an amino-polymer, which comprises reacting tannin or said hydroxy- or amino-polymer with cyanogen halide, epihalohydrin, α,ω-bis( 2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin or polymer and reacting said activated tannin with said hydroxy- or amino-polymer; or reacting said activated polymer with tannin.
Claim 31
Independent Claim
38 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic amino-polymer, which comprises diazotizing said amino-polymer to give a diazotized amino-polymer, and reacting the diazotized amino-polymer with tannin.
Claim 32
Independent Claim
38 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic amino-polymer, which comprises diazotizing said amino-polymer to give a diazotized amino-polymer, and reacting the diazotized amino-polymer with tannin.
Claim 33
Independent Claim
114 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer and an amino-polymer, which comprises: (A) reacting tannin with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin, reacting said activated tannin with alkylenediamine or aminoalkanol to give an aminoalkyl- or hydroxyalkyl-tannin, and (B) reacting any of said aminoalkyl-tannin, hydroxyalkyl-tannin, the hydroxy-polymer or the amino-polymer with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane, α,ω-bis(2,3-epoxypropoxy)alkane or haloalkanoly halide to give an activated tannin derivative or polymer, and reacting said activated tannin derivative with said hydroxy- or amino-polymer; or reacting said activated polymer with said aminoalkyl- or hydroxyalkyl-tannin.
Claim 34
Independent Claim
114 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of a hydroxy-polymer and an amino-polymer, which comprises: (A) reacting tannin with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin, reacting said activated tannin with alkylenediamine or aminoalkanol to give an aminoalkyl- or hydroxyalkyl-tannin, and (B) reacting any of said aminoalkyl-tannin, hydroxyalkyl-tannin, the hydroxy-polymer or the amino-polymer with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane, α,ω-bis(2,3-epoxypropoxy)alkane or haloalkanoly halide to give an activated tannin derivative or polymer, and reacting said activated tannin derivative with said hydroxy- or amino-polymer; or reacting said activated polymer with said aminoalkyl- or hydroxyalkyl-tannin.
Claim 35
Independent Claim
79 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of an amino-polymer and a carboxyl-polymer, which comprises: (A) reacting tannin with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin, reacting said activated tannin with alkylenediamine or amino-carboxylic acid to give an aminoalkyl- or carboxyalkyl-tannin, and (B) reacting the aminoalkyl-tannin with the carboxyl-polymer; or reacting the carboxyalkyl-tannin with the amino-polymer.
Claim 36
Independent Claim
79 words
A method of preparing a water-insoluble tannin preparation consisting essentially of tannin bound by covalent linkage to a water-insoluble, hydrophilic carrier selected from the group consisting of an amino-polymer and a carboxyl-polymer, which comprises: (A) reacting tannin with cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane or α,ω-bis(2,3-epoxypropoxy)alkane to give an activated tannin, reacting said activated tannin with alkylenediamine or amino-carboxylic acid to give an aminoalkyl- or carboxyalkyl-tannin, and (B) reacting the aminoalkyl-tannin with the carboxyl-polymer; or reacting the carboxyalkyl-tannin with the amino-polymer.
Claim 37
Dependent Claim
19 words
The method of claim 20 wherein the hydroxy-polymer is polysaccharide or hydroxyalkyl-polysaccharide, and the amino-polymer is aminoalkyl-polysaccharide or aminobenzyl-polysaccharide.
Claim 38
Dependent Claim
10 words
The method of claim 21 wherein the amino-polymer is aminobenzyl-polysaccharide.
Claim 39
Dependent Claim
19 words
The method of claim 22 wherein the hydroxy-polymer is polysaccharide or hydroxyalkyl-polysaccharide, and the amino-polymer is aminoalkyl-polysaccharide or aminobenzyl-polysaccharide.
Claim 40
Dependent Claim
17 words
The method of claim 23 wherein the amino-polymer is aminoalkyl-polysaccharide or aminobenzyl-polysaccharide, and the carboxyl-polymer is carboxyalkyl-polysaccharide.
Claim 41
Dependent Claim
11 words
The method of claim 20 wherein said tannin is pyrogallol tannin.
Claim 42
Dependent Claim
11 words
The method of claim 21 wherein said tannin is pyrogallol tannin.
Claim 43
Dependent Claim
11 words
The method of claim 22 wherein said tannin is pyrogallol tannin.
Claim 44
Dependent Claim
11 words
The method of claim 23 wherein said tannin is pyrogallol tannin.
Claim 45
Dependent Claim
57 words
An immobilized protein consisting essentially of a biologically active protein adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane.
Claim 46
Dependent Claim
57 words
An immobilized protein consisting essentially of a biologically active protein adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane.
Claim 47
Dependent Claim
31 words
The immobilized protein of claim 32 wherein said cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)-alkane and α,ω-bis(2,3-epoxypropoxy)alkane are employed in combination with at least one of alkylenediamine, aminoalkanol, aminoalkanoic acid, alkylenediol and haloalkanoyl halide.
Claim 48
Dependent Claim
146 words
An immobilized protein consisting essentially of a catalytically active enzyme adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6.
Claim 49
Dependent Claim
146 words
An immobilized protein consisting essentially of a catalytically active enzyme adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6.
Claim 50
Dependent Claim
160 words
An immobilized protein consisting essentially of a catalytically active enzyme adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR18## and ##STR19## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6.
Claim 51
Dependent Claim
160 words
An immobilized protein consisting essentially of a catalytically active enzyme adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR18## and ##STR19## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6.
Claim 52
Dependent Claim
90 words
An immobilized protein consisting essentially of a biologically active protein adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin bound by physical adsorption to a polysaccharide derivative taken from the class consisting of: Polysaccharide--O--CH 2 CH(OH)CH 2 O(CH 2 ) m H, Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H, Polysaccharide--O--CONH(CH 2 ) m H, ##STR20## Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 , and Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16.
Claim 53
Dependent Claim
90 words
An immobilized protein consisting essentially of a biologically active protein adsorbed physically by a water-insoluble tannin preparation, said preparation consisting essentially of tannin bound by physical adsorption to a polysaccharide derivative taken from the class consisting of: Polysaccharide--O--CH 2 CH(OH)CH 2 O(CH 2 ) m H, Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H, Polysaccharide--O--CONH(CH 2 ) m H, ##STR20## Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 , and Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16.
Claim 54
Independent Claim
83 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
Claim 55
Independent Claim
83 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a diazo linkage or by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
Claim 56
Independent Claim
95 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer, by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane in combination with at least one of alkylenediamine, aminoalkanol, aminoalkanoic acid, alkylenediol and haloalkanoyl halide, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
Claim 57
Independent Claim
95 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer, by means of at least one of cyanogen halide, epihalohydrin, α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropyl)alkane and α,ω-bis(2,3-epoxypropoxy)alkane in combination with at least one of alkylenediamine, aminoalkanol, aminoalkanoic acid, alkylenediol and haloalkanoyl halide, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
Claim 58
Independent Claim
172 words
A method of preparing an immobilized protein which comprises: (A) contacting a catalytically active enzyme with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n --NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6, (B) permitting said enzyme to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized enzyme.
Claim 59
Independent Claim
172 words
A method of preparing an immobilized protein which comprises: (A) contacting a catalytically active enzyme with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group consisting of --CONH--, --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n --NHCO--, --Y'--(CH 2 ) n --CONH--, --Y'--(CH 2 ) n --NHCH 2 CH(OH)CH 2 --, --Y'--(CH 2 ) n NH(CH 2 ) m CO-- and --CONH(CH 2 ) n OCH 2 CH(OH)CH 2 --, wherein Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, each one of n and m is an integer of 1 to 16 and q is an integer of 1 to 6, (B) permitting said enzyme to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized enzyme.
Claim 60
Independent Claim
186 words
A method of preparing an immobilized protein which comprises: (A) contacting a catalytically active enzyme with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group of consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR21## and ##STR22## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6, (B) permitting said enzyme to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized enzyme.
Claim 61
Independent Claim
186 words
A method of preparing an immobilized protein which comprises: (A) contacting a catalytically active enzyme with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin covalently bound to a water-insoluble, hydrophilic carrier selected from a hydroxy-polymer, an amino-polymer and a carboxyl-polymer through a linkage selected from the group of consisting of --CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 --A--CH 2 CH(OH)CH 2 --, --CH 2 CH(OH)CH 2 NH(CH 2 ) n --Y--, --CONH(CH 2 ) n --Y--, --Y'--(CH 2 ) n CONH(CH 2 ) m --Y--, --Y'--(CH 2 ) n NHCO(CH 2 ) m --Y--, ##STR21## and ##STR22## wherein Y is --NHCO--, --NHCH 2 CH(OH)CH 2 --, --OCH 2 CH(OH)CH 2 -- or --NHCOCH 2 --, Y' is --CONH-- or --CH 2 CH(OH)CH 2 NH--, each one of n and m is an integer of 1 to 16, A is --(CH 2 ) q -- or --O(CH 2 ) q O--, and q is an integer of 1 to 6, (B) permitting said enzyme to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized enzyme.
Claim 62
Independent Claim
117 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin bound by physical adsorption to a water-insoluble, hydrophilic carrier selected from the group consisting of: Polysaccharide--O--CH 2 CH(OH)CH 2 O(CH 2 ) m H, Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H, Polysaccharide--O--CONH(CH 2 ) m H, ##STR23## Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 , and Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
Claim 63
Independent Claim
117 words
A method of preparing an immobilized protein which comprises: (A) contacting a biologically active protein with a water-insoluble tannin preparation in an aqueous solvent, said tannin preparation consisting essentially of tannin bound by physical adsorption to a water-insoluble, hydrophilic carrier selected from the group consisting of: Polysaccharide--O--CH 2 CH(OH)CH 2 O(CH 2 ) m H, Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m H, Polysaccharide--O--CONH(CH 2 ) m H, ##STR23## Polysaccharide--O--CH 2 CH(OH)CH 2 NH(CH 2 ) m NH 2 , and Polysaccharide--O--CH 2 CONH(CH 2 ) m NH 2 wherein m is an integer of 1 to 16, (B) permitting said protein to be adsorbed on said tannin preparation, and (C) recovering the resultant immobilized protein.
References Cited (Backward Citations)
US2650194A
Cited
US3639558A
Cited
US3796634A
Cited
US3736231A
Cited
US3886066A
Cited
US4008339A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-22 | 1981-11-21 | 1982-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-22 | 1985-11-21 | 1986-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-22 | 1989-11-21 | 1990-05-23 | $7700.00 | Window Closed / Lapsed |