US3821087A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture on semi-permeable tubular membranes
Assignee: United States Department of Health and Human Services
•
Filing: 1972-05-18
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Granted: 1974-06-28
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Expires: 1992-05-18
Abstract
Abstract The growth of cells in vitro on semi-permeable, tube-shaped membranes or capillaries is disclosed. Cells are initially allowed to settle onto the outer surfaces of the membranes in a nutrient medium environment while the membranes are continuously perfused by oxygenated nutrient medium flowing through the membranes. Nutrient substances diffuse from the perfusing medium through the membrane wall and into the cells, while cell products diffuse from the cell through the membrane wall into the perfusate from which the cell products may be recovered.
Inventors
- 👤 R Knazek
- 👤 P Gullino
- 👤 W Kidwell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:40
CPC Classifications
C; C12; C12M; C12M25/00; C12M25/10; C12M29/00; C12M29/10; C12M29/16
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
27 words
The cell culture unit of claim 1 including capillaries having walls formed of different semi-permeable materials, some of said capillaries being more permeable to gases than others.
Claim 2
Dependent Claim
29 words
The cell culture unit of claim 1 wherein some of said capillaries have walls formed of cellulose acetate and some of said capillaries have walls formed of silicone polycarbonate.
Claim 3
Dependent Claim
48 words
Apparatus for the formation and maintenance of solid tissues in vitro comprising, in combination, at least one cell culture unit according to claim 1, a reservoir for perfusate, pump means, conduit means interconnecting said reservoir with said pump means and said pump means with said cell culture unit.
Claim 4
Dependent Claim
14 words
The apparatus of claim 4 including, in combination, a plurality of cell culture units.
Claim 5
Dependent Claim
29 words
The apparatus of claim 4 further including, in combination, means for exposing the perfusate to oxygen and carbon dioxide prior to pumping the perfusate through said cell culture unit.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-27 | 1977-12-27 | 1978-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-27 | 1981-12-27 | 1982-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-27 | 1985-12-27 | 1986-06-28 | $7700.00 | Window Closed / Lapsed |