US4078971A
Expired / Lapsed
US
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Bound, active cellular organelles and method of producing same
Assignee: Temple University
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Filing: 1976-03-31
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Granted: 1978-03-14
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Expires: 1996-03-31
Abstract
Abstract Cellular organelles, examples of which are mitochondria, microsomes and chloroplasts, are bound in an active state on a substantially inert and insoluble support surface, examples of which are alkylated glass, kaolin, talc, silica, ferrites, alumina, and some alkyl substituted high molecular weight polymers. Binding between the support surface and the organelle is accomplished by adsorption of the isolated organelle onto the support surface. Immobilization in this manner does not affect the functional behavior of the organelle. These systems have utility for the synthesis of specific chemical substances.
Inventors
- 👤 Barry Arkles
- 👤 William S. Brinigar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 07:53
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12N11/02; C12N11/08; C12N11/089
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
42 words
Cellular organelles, from the group consisting of mitochondria, microsomes and chloroplasts, bound and immobilized in an active state on a substantially inert and insoluble solid substrate from the group consisting of glass, kaolin, talc, silica, ferrite, alumina and high molecular weight polymers.
Claim 2
Dependent Claim
42 words
Cellular organelles, from the group consisting of mitochondria, microsomes and chloroplasts, bound and immobilized in an active state on a substantially inert and insoluble solid substrate from the group consisting of glass, kaolin, talc, silica, ferrite, alumina and high molecular weight polymers.
Claim 3
Dependent Claim
17 words
Bound cellular organelles, as recited in claim 1, wherein said organelles are hydrophobically bound on said substrate.
Claim 4
Dependent Claim
19 words
Bound cellular organelles, as recited in claim 2, wherein said support includes a residue of an uncharged alkyl silane.
Claim 5
Dependent Claim
25 words
Bound cellular organelles, as recited in claim 2, wherein said support is in the form of beads in the range of 100 - 150 mesh.
Claim 6
Dependent Claim
22 words
Bound cellular organelles, as recited in claim 3, wherein said alkyl substituent of said silane includes from two to twenty-four carbon atoms.
Claim 7
Dependent Claim
51 words
Bound cellular organelles, as recited in claim 3, wherein said alkyl silane is of the general structure R n SiX 4-n where n = 1 to 3, X is a labile substituent selected from the group consisting of halogen and alkoxy, and R is hydrocarbon of 2 to 24 carbon atoms.
Claim 8
Dependent Claim
13 words
Bound cellular organelles, as recited in claim 1, wherein said organelles are mitochondria.
Claim 9
Dependent Claim
13 words
Bound cellular organelles, as recited in claim 1, wherein said organelles are chloroplasts.
Claim 10
Dependent Claim
13 words
Bound cellular organelles, as recited in claim 1, wherein said organelles are microsomes.
Claim 11
Dependent Claim
15 words
Bound cellular organelles, as recited in claim 2, wherein said support comprises porous silica beads.
Claim 12
Dependent Claim
25 words
A process for producing desired biological chemical substances by bringing reactive precursor chemical substances into contact with a bound organelle as recited in claim 1.
Claim 13
Dependent Claim
107 words
Method of binding sub-cellular organelles in an active state on an inert solid support comprising: (a) isolating organelles selected from the group consisting of mitochondria, microsomes and chloroplasts and removing substantially all excess lipid therefrom; (b) preparing an inert support from the group consisting of glass, kaolin, talc, silica, ferrite, alumina and high molecular weight polymers, by treating said surface with a hydrophobic coupling agent; and (c) contacting said prepared inert solid support material with said organelle, free of excess lipid, and permitting said support material and said organelles to remain in contact until a significant amount of said organelles are bound and immobilized on said substrate.
Claim 14
Dependent Claim
107 words
Method of binding sub-cellular organelles in an active state on an inert solid support comprising: (a) isolating organelles selected from the group consisting of mitochondria, microsomes and chloroplasts and removing substantially all excess lipid therefrom; (b) preparing an inert support from the group consisting of glass, kaolin, talc, silica, ferrite, alumina and high molecular weight polymers, by treating said surface with a hydrophobic coupling agent; and (c) contacting said prepared inert solid support material with said organelle, free of excess lipid, and permitting said support material and said organelles to remain in contact until a significant amount of said organelles are bound and immobilized on said substrate.
Claim 15
Dependent Claim
49 words
Method as recited in claim 12, wherein said hydrophobic coupling agent is an alkylsilane of the general structure R n SiX 4-n where n = 1 to 3, X is selected from the group consisting of halogen and alkoxy, and R is hydrocarbon of 2 to 24 carbon atoms.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-03-13 | 1981-09-12 | 1982-03-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-03-13 | 1985-09-12 | 1986-03-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-03-13 | 1989-09-12 | 1990-03-14 | $7700.00 | Window Closed / Lapsed |