Patent US4024020A successfully refreshed from Google Patents.
US4024020A
Expired / Lapsed
US
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Method of cell culture on polyacrylonitrile surface
Assignee: Monsanto Co
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Filing: 1975-12-15
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Granted: 1977-05-17
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Expires: 1995-12-15
Abstract
Abstract A method of culturing cells in which the cells are fixed on a polyacrylonitrile surface.
Inventors
- 👤 Stefan A. Weiss
- 👤 John H. Johnson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:36
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/50
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
49 words
In a method for culture of the type of cells which grow attached to a surface, the improvement comprising fixing the cells to be cultivated to a polyacrylonitrile surface maintained in contact with a nutrient culture medium, said polyacrylonitrile comprising acrylonitrile with 0 to 15% by weight of comonomer.
Claim 2
Dependent Claim
49 words
In a method for culture of the type of cells which grow attached to a surface, the improvement comprising fixing the cells to be cultivated to a polyacrylonitrile surface maintained in contact with a nutrient culture medium, said polyacrylonitrile comprising acrylonitrile with 0 to 15% by weight of comonomer.
Claim 3
Dependent Claim
12 words
The method of claim 1 in which the cells are mammalian cells.
Claim 4
Dependent Claim
12 words
The method of claim 1 in which the cells are diploid cells.
Claim 5
Dependent Claim
13 words
The method of claim 1 in which the cells are normal human cells.
Claim 6
Dependent Claim
14 words
The method of claim 1 in which the surface is a homopolymer of acrylonitrile.
Claim 7
Independent Claim
62 words
A method for propagating or maintaining cells in vitro which comprises providing a suspension of cells in a culture medium and contacting such medium with a polymeric surface suitable for cell attachment and growth which is a polymer of acrylonitrile with up to 15% by weight of a vinyl comonomer, and incubating under cell growth or maintenance conditions of pH and temperature.
Claim 8
Independent Claim
62 words
A method for propagating or maintaining cells in vitro which comprises providing a suspension of cells in a culture medium and contacting such medium with a polymeric surface suitable for cell attachment and growth which is a polymer of acrylonitrile with up to 15% by weight of a vinyl comonomer, and incubating under cell growth or maintenance conditions of pH and temperature.
Claim 9
Dependent Claim
17 words
The method of claim 6 in which the polymer is a copolymer of acrylonitrile and vinyl acetate.
Claim 10
Dependent Claim
24 words
The method of claim 7 in which the polymer is a copolymer of about 95% by weight acrylonitrile and 5% by weight vinyl acetate.
References Cited (Backward Citations)
US3873423A
Cited
US3850748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-16 | 1980-11-15 | 1981-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-16 | 1984-11-15 | 1985-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-16 | 1988-11-15 | 1989-05-17 | $7700.00 | Window Closed / Lapsed |