Patent US4020151A successfully refreshed from Google Patents.
US4020151A
Expired / Lapsed
US
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Method for quantitation of antigens or antibodies on a solid surface
Assignee: Lonza Walkersville Inc
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Filing: 1976-02-17
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Granted: 1977-04-26
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Expires: 1996-02-17
Abstract
Abstract A method for quantitation of antigens or antibodies from a liquid sample on a solid surface. The sample is first sorbed directly to a nonimmunological surface. Then the surface is exposed to antibody or antigen labelled preferably with a fluorogen, to cause an immunological reaction to occur. After removal of unreacted reagent from the surface, the quantity of reacted labelled reagent is determined, as for example, fluorogen by a fluorometer. When the sample includes significant quantities of protein other than the antigen or antibody to be quantitated, buffer protein unreactive with the labelled antigen or antibody is added in concentrations sufficient to minimize the dependence of sorbed antigen or antibody upon variations in the concentration of such other protein.
Inventors
- 👤 Gunner Bolz
- 👤 Fred H. Deindoerfer
- 👤 Chun P. Hu
- 👤 Naomi Kameda
- 👤 Robert Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:34
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N33/53; G01N33/543; G01N33/54306; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
96 words
In a method for quantitation of antigens or antibodies in a liquid sample, the steps of a. sorbing said sample antibody or antigen directly onto an immunologically unreactive solid support surface sorptive for said sample antibody or antigen, b. exposing the sorbed surface to an aqueous reagent including an excess of labelled specific antibody or antigen immunologically reactive with said sample antigen or antibody to cause said immunological reaction to occur on said surface, c. removing the unreacted reagent from said surface, and d. measuring the quantity of reacted labelled antigen or antibody on said surface.
Claim 2
Dependent Claim
96 words
In a method for quantitation of antigens or antibodies in a liquid sample, the steps of a. sorbing said sample antibody or antigen directly onto an immunologically unreactive solid support surface sorptive for said sample antibody or antigen, b. exposing the sorbed surface to an aqueous reagent including an excess of labelled specific antibody or antigen immunologically reactive with said sample antigen or antibody to cause said immunological reaction to occur on said surface, c. removing the unreacted reagent from said surface, and d. measuring the quantity of reacted labelled antigen or antibody on said surface.
Claim 3
Dependent Claim
16 words
The method of claim 1 in which said sample is physically adsorbed upon said support surface.
Claim 4
Dependent Claim
23 words
The method of claim 1 in which said support surface comprises ion exchange resin and said sample is ionically bound to said resin.
Claim 5
Dependent Claim
35 words
The method of claim 1 in which the labelled portion of said labelled antibody or antigen is selected from the group consisting of a luminescent substance, a radioactive substance, an enzyme or metal containing substance.
Claim 6
Dependent Claim
19 words
The method of claim 1 in which the labelled portion of said labelled antibody or antigen comprises a fluorogen.
Claim 7
Dependent Claim
21 words
The method of claim 5 in which said reaction support surface is read fluorometrically in a window framed by opaque material.
Claim 8
Dependent Claim
88 words
The method of claim 1 in which said sample includes significant quantities of protein other than the antigen or antibody to be quantitated, said method including the step of adding to said sample prior to step (a) a predetermined quantity of buffer protein unreactive with said labelled antigen or antibody at a concentration in excess of the antigen or antibody concentration of said sample to minimize the dependence of sorbed antibody or antigen to be quantitated upon variations in the content of said other protein in said sample.
Claim 9
Dependent Claim
26 words
The method of claim 3 in which the ratio of said buffer protein to total protein of said sample is in excess of 1:1 by weight.
Claim 10
Dependent Claim
31 words
The method of claim 1 in which said support surface is selected from the group consisting of a polymeric substrate, silica gel, silicone wafers, glass, insoluble proteins, and a metallic surface.
Claim 11
Dependent Claim
26 words
The method of claim 1 in which said sample antigen or antibody or step (a) comprises antigen and said labelled antibody or antigen comprises labelled antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-25 | 1980-10-25 | 1981-04-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-25 | 1984-10-25 | 1985-04-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-25 | 1988-10-25 | 1989-04-26 | $7700.00 | Window Closed / Lapsed |