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US4010250A
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Radioactive iodine (125I) labeling of latex particles
Assignee: United States Department of the Navy
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Filing: 1975-03-06
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Granted: 1977-03-01
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Expires: 1995-03-06
Abstract
Abstract The invention disclosed in this application is directed towards developing a radioiodination method which is applicable to the labeling of 2.02 micrometer ( mu m) and 0.37 micrometer ( mu m) diameter polyvinyltoluene latex particles that have been used as an immunoadsorbent. More particularly the overall method includes using an oxidation-reduction chemical reaction for tagging latex particles. Two methods are described. One, the hydrochloric acid method; and two, the nitric acid method.
Inventors
- 👤 Gokaldas C. Parikh
- 👤 Chi Kuan Ho
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534; G21; G21H; G21H5/00; G21H5/02
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
203 words
The method of preparing radioactive iodine 125 labeled latex particles of polyvinyl toluene in a predetermined size range of 2.02 μm to 0.37 μm in diameter comprising the following steps: Step 1, prepare a first solution which contains 1 ml of 1 N nitric acid, 2 drops of concentrated hydrogen peroxide, 0.02 ml of 0.02 N potassium iodide, 2 ml of boric acid buffer at pH 8.0, and 0.05 mCi of carrier-free 125 I; Step 2, prepare a second solution which contains 0.1 ml of 1:200 dilution polyvinyltoluene latex, 5 mg of sodium lauryl sulfate and 2.4 ml of dionized water; Step 3, add a drop of 0.1 N sodium nitrite to said first chemical solution; Step 4, transfer said first chemical solution to said second chemical solution in a drop by drop process, said solution being stirred constantly for a predetermined period of time; Step 5, add 1.02 ml of 1 N sodium hydroxide; Step 6, add the material thus compounded to a dialysis bag by a very slow drop by drop process, said material being maintained in said dialysis bag up to 36 hours while suspended in a dialysis solution of boric acid, said dialysis solution being periodically changed and analyzed;
Claim 2
Independent Claim
203 words
The method of preparing radioactive iodine 125 labeled latex particles of polyvinyl toluene in a predetermined size range of 2.02 μm to 0.37 μm in diameter comprising the following steps: Step 1, prepare a first solution which contains 1 ml of 1 N nitric acid, 2 drops of concentrated hydrogen peroxide, 0.02 ml of 0.02 N potassium iodide, 2 ml of boric acid buffer at pH 8.0, and 0.05 mCi of carrier-free 125 I; Step 2, prepare a second solution which contains 0.1 ml of 1:200 dilution polyvinyltoluene latex, 5 mg of sodium lauryl sulfate and 2.4 ml of dionized water; Step 3, add a drop of 0.1 N sodium nitrite to said first chemical solution; Step 4, transfer said first chemical solution to said second chemical solution in a drop by drop process, said solution being stirred constantly for a predetermined period of time; Step 5, add 1.02 ml of 1 N sodium hydroxide; Step 6, add the material thus compounded to a dialysis bag by a very slow drop by drop process, said material being maintained in said dialysis bag up to 36 hours while suspended in a dialysis solution of boric acid, said dialysis solution being periodically changed and analyzed;
Claim 3
Dependent Claim
20 words
Step 7, said material being removed from said dialysis bag for use subsequently in the process of tagging certain virus.
Claim 4
Dependent Claim
20 words
Step 7, said material being removed from said dialysis bag for use subsequently in the process of tagging certain virus.
Claim 5
Independent Claim
105 words
The method of preparing radioactive iodine 125 labeled latex particles of polyvinyltoluene in a predetermined size range of 2.02 μm to 0.37 μm in diameter comprisng the following steps: Step 1, preparing a first solution of 1 ml of 1 M sodium hydroxide in 2 ml of boric acid buffer at pH 8.0; Step 2, preparing a second solution containing 1 ml of 1 M HCl, 0.02 ml of 0.02 N potassium iodide and 0.1 mCi of carrier-free 125 I; Step 3, preparing a third solution which contains 0.1 ml of 1:200 dilution latex suspended in 2.4 ml of boric acid buffer at pH 8.0;
Claim 6
Independent Claim
105 words
The method of preparing radioactive iodine 125 labeled latex particles of polyvinyltoluene in a predetermined size range of 2.02 μm to 0.37 μm in diameter comprisng the following steps: Step 1, preparing a first solution of 1 ml of 1 M sodium hydroxide in 2 ml of boric acid buffer at pH 8.0; Step 2, preparing a second solution containing 1 ml of 1 M HCl, 0.02 ml of 0.02 N potassium iodide and 0.1 mCi of carrier-free 125 I; Step 3, preparing a third solution which contains 0.1 ml of 1:200 dilution latex suspended in 2.4 ml of boric acid buffer at pH 8.0;
Claim 7
Dependent Claim
91 words
Step 4, add a drop of 0.1 N sodium nitrite to said first solution and stir; Step 5, combine the first and second solutions; Step 6, add the thus produced solution to the third solution in a drop by drop process and stir for at least 10 minutes; Step 7, pipette the material thus compounded into a dialysis bag; Step 8, maintain said material in said dialysis bag up to 36 hours while it is suspended in a dialysis solution of sodium sulfate, said dialysis solution being periodically changed and analyzed.
Claim 8
Dependent Claim
91 words
Step 4, add a drop of 0.1 N sodium nitrite to said first solution and stir; Step 5, combine the first and second solutions; Step 6, add the thus produced solution to the third solution in a drop by drop process and stir for at least 10 minutes; Step 7, pipette the material thus compounded into a dialysis bag; Step 8, maintain said material in said dialysis bag up to 36 hours while it is suspended in a dialysis solution of sodium sulfate, said dialysis solution being periodically changed and analyzed.
References Cited (Backward Citations)
US3039977A
Cited
US3853987A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1980-02-29 | 1980-08-30 | 1981-03-01 | $2000.00 | Window Closed / Lapsed |
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