US4006059A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hydrophobic noncovalent binding of proteins to support materials
Assignee: Purdue Research Foundation
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Filing: 1974-07-29
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Granted: 1977-02-01
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Expires: 1994-07-29
Abstract
Abstract Proteins such as enzymes are noncovalently immobilized by treating a hydrophilic support material having hydroxyl or amino groups with an acid chloride of a hydrophobic acid such as phenoxyacetyl chloride and to the resultant hydrophobic support material adsorbing a protein.
Inventors
- 👤 Larry G. Butler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:50
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; C08; C08B; C08B3/00; C08B3/22; C12N11/14; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
127 words
A method of noncovalent binding of proteins to support materials, said method comprising: selecting a hydrophilic support material having hydroxyl or amino groups; treating said selected support material by chemically modifying said material by treatment with phenoxyacetyl chloride to make said support material hydrophobic in nature to thus form a hydrophobic derivative of said support material suitable for immobilizing proteins when exposed to said treated hydrophobic material; selecting a protein that is capable of being immobilized on said treated hydrophobic material with said protein having a capacity for subsequent biological activity; and exposing said selected protein to said treated hydrophobic material to cause said protein to be effectively immobilized on said treated hydrophobic material by adsorption and substantially without impairing said subsequent biological activity of the protein.
Claim 2
Independent Claim
127 words
A method of noncovalent binding of proteins to support materials, said method comprising: selecting a hydrophilic support material having hydroxyl or amino groups; treating said selected support material by chemically modifying said material by treatment with phenoxyacetyl chloride to make said support material hydrophobic in nature to thus form a hydrophobic derivative of said support material suitable for immobilizing proteins when exposed to said treated hydrophobic material; selecting a protein that is capable of being immobilized on said treated hydrophobic material with said protein having a capacity for subsequent biological activity; and exposing said selected protein to said treated hydrophobic material to cause said protein to be effectively immobilized on said treated hydrophobic material by adsorption and substantially without impairing said subsequent biological activity of the protein.
Claim 3
Dependent Claim
23 words
The method of claim 1 wherein said method includes the step of selecting said hydrophilic support material from carbohydrate polymers and inorganic materials.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein said carbohydrate polymer is cellulose.
Claim 5
Dependent Claim
11 words
The method of claim 2 wherein said inorganic material is glass.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein said method includes exposing said protein to said support material in an aqueous environment.
References Cited (Backward Citations)
US3909360A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-01 | 1980-08-02 | 1981-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-01 | 1984-08-02 | 1985-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-01 | 1988-08-02 | 1989-02-01 | $7700.00 | Window Closed / Lapsed |