US3909360A
Expired / Lapsed
US
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Process for producing insolubilized enzyme
Assignee: Toyo Jozo KK
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Filing: 1974-03-18
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Granted: 1975-09-30
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Expires: 1994-03-18
Abstract
Abstract Enzymes acting on glycerides or glycero-phosphatides, for example, lipase, phospholipase, or phosphatidate phosphatase are insolubilized by merely contacting an aqueous enzyme solution with a carrier selected from fatty acid esters of water-insoluble polysaccharides or derivatives thereof containing hydroxyl groups.
Inventors
- 👤 Yoshifumi Horiuchi
- 👤 Shigeyuki Imamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:19
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/64; C12P7/6409; C12P7/6418; C12N; C12N11/00; C12N11/02; C12N11/10; C12N9/00; C12N9/14; C12N9/16; C12N9/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/834; Y10S435/886
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
24 words
A PROCESS FOR PODUCING INSOLUBILIZED ENZYME, WHICH COMPRISES CONTACTING AN AQUEOUS SOLUTION OF AN ENZYME HAVING SUBSTRATE SPECIFICALLY FOR GLYCERO-PHOSPHATIDES REPRESENTED BY THE FORMULA:
Claim 2
Dependent Claim
24 words
A PROCESS FOR PODUCING INSOLUBILIZED ENZYME, WHICH COMPRISES CONTACTING AN AQUEOUS SOLUTION OF AN ENZYME HAVING SUBSTRATE SPECIFICALLY FOR GLYCERO-PHOSPHATIDES REPRESENTED BY THE FORMULA:
Claim 3
Dependent Claim
11 words
A process according to claim 1, wherein the enzyme is lipase.
Claim 4
Dependent Claim
11 words
A process according to claim 1, wherein the enzyme is phospholipase.
Claim 5
Dependent Claim
12 words
A process according to claim 1, wherein the enzyme is phosphatidate phosphatase.
Claim 6
Dependent Claim
29 words
A process according to claim 1, wherein the contacting of said enzyme with said carrier is performed at 20* to 30*C. and at a pH of 7 to 8.
Claim 7
Dependent Claim
16 words
A process according to claim 1, wherein the carrier is pre-treated with a hydrophilic organic solvent.
Claim 8
Dependent Claim
16 words
A process according to claim 6, wherein the organic solvent is acetone or a lower alcohol.
Claim 9
Dependent Claim
18 words
A process according to claim 1, further comprising separating the enzyme insolubilized on said carrier from said solution.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-29 | 1979-03-31 | 1979-09-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-29 | 1983-03-31 | 1983-09-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-29 | 1987-03-31 | 1987-09-30 | $7700.00 | Window Closed / Lapsed |