Patent US4104725A successfully refreshed from Google Patents.
US4004979A
Expired / Lapsed
US
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Preparation of active proteins cross-linked to inactive proteins
Assignee: Bpifrance Financement SA
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Filing: 1975-01-15
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Granted: 1977-01-25
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Expires: 1995-01-15
Abstract
Abstract Articles containing active proteins, such as enzymes, crosslinked to an inactive protein are prepared by dispersing and reacting in a solvent an active protein and an inactive protein simultaneously with a crosslinking agent to crosslink the active and inactive proteins together. The degree of crosslinking can be controlled by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth. Optionally, an inert carrier may be present during crosslinking. The resultant crosslinked active and inactive proteins can be in various forms such as solutions, suspensions, gels, films, membranes, granules, pills or tablets.
Inventors
- 👤 Stratis Avrameas
- 👤 Georges Broun
- 👤 Eric Selegny
- 👤 Daniel Thomas
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:43
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K38/00; A61K38/16; A61K38/17; A61K38/36; A61K38/363; A61K38/38; A61K38/43; A61K38/44; A61K38/443; A61K38/46; A61K38/48; A61K38/482; A61K38/4826; A61K38/51; A61K39/00; A61K39/395; A61K39/44; C12N11/14; Y; Y10; Y10S; Y10S436/00; Y10S436/823; Y10S530/00; Y10S530/81; Y10S530/812
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
131 words
A process for producing an article containing a cross-linked active protein substance which comprises dispersing, mixing and reacting in a solvent medium 1. an active protein and 2. an inactive protein simultaneously with 3. a cross-linking agent for said proteins under cross-linking conditions for said cross-linking agent until an active protein substance is formed containing said active protein cross-linked with said inactive protein substance, controlling the degree of cross-linking by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth, said formed active protein substance amounting to about 1 to 20% by weight of the total proteinic substances and said cross-linking agent amounting to about 0.5 to 8% weight based on the weight of the whole mixture being reacted and separating the solvent from said formed active protein substance.
Claim 2
Dependent Claim
131 words
A process for producing an article containing a cross-linked active protein substance which comprises dispersing, mixing and reacting in a solvent medium 1. an active protein and 2. an inactive protein simultaneously with 3. a cross-linking agent for said proteins under cross-linking conditions for said cross-linking agent until an active protein substance is formed containing said active protein cross-linked with said inactive protein substance, controlling the degree of cross-linking by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth, said formed active protein substance amounting to about 1 to 20% by weight of the total proteinic substances and said cross-linking agent amounting to about 0.5 to 8% weight based on the weight of the whole mixture being reacted and separating the solvent from said formed active protein substance.
Claim 3
Dependent Claim
17 words
The process of claim 1 wherein said cross-linked active protein substance is positioned on a hydrophobic carrier.
Claim 4
Dependent Claim
13 words
The process of claim 2 wherein the hydrophobic carrier is a silicone membrane.
References Cited (Backward Citations)
US3639558A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-25 | 1980-07-26 | 1981-01-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-25 | 1984-07-26 | 1985-01-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-25 | 1988-07-26 | 1989-01-25 | $7700.00 | Window Closed / Lapsed |