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US3984533A
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US
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Electrophoretic method of detecting antigen-antibody reaction
Assignee: General Electric Co
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Filing: 1975-11-13
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Granted: 1976-10-05
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Expires: 1995-11-13
Abstract
Abstract A method of rapidly detecting presence of antibodies in a solution comprises depositing, on each of a plurality of microscopic particles, an antigen specific to the antibodies sought, and forming a dilute suspension of the particles in the solution to be examined. The suspension is stirred, and electrophoretic mobility of the particles is measured upon formation of the suspension and measured again at a subsequent time. Detection of a change in electrophoretic mobility of the particles between the two measurements indicates presence of the antibodies in the solution.
Inventors
- 👤 Egidijus E. Uzgiris
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:23
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
60 words
The method of detecting an antigen-antibody reaction comprising the steps of: depositing an antigen on each of a plurality of microscopic particles; forming a dilute suspension of said particles in a solution to be tested for presence of antibodies specific to the antigens on said particles; stirring said suspension; and comparing electrophoretic mobilities of said particles at two different times.
Claim 2
Independent Claim
60 words
The method of detecting an antigen-antibody reaction comprising the steps of: depositing an antigen on each of a plurality of microscopic particles; forming a dilute suspension of said particles in a solution to be tested for presence of antibodies specific to the antigens on said particles; stirring said suspension; and comparing electrophoretic mobilities of said particles at two different times.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein the step of stirring of said suspension comprises mixing said solution by electrophoretic motion.
Claim 4
Dependent Claim
21 words
The method of claim 1 wherein the step of stirring said suspension comprises mixing said solution turbulently and by electrophoretic motion.
Claim 5
Dependent Claim
78 words
The method of claim 1 wherein the step of comparing mobilities of said particles at two different times comprises detecting, at a predetermined scattering angle, laser light scattered by said particles and by a fixed light scatterer at each of said different times, analyzing the heterodyne product of the frequencies detected at each of said different times, and comparing the analyzed heterodyne products to obtain a measure of the changed mobilities of said particles between said different times.
Claim 6
Independent Claim
62 words
The method of detecting a reaction between a first protein and a second protein comprising the steps of: depositing said first protein on each of a plurality of microscopic particles; forming a dilute suspension of said particles in a solution to be tested for presence of said second protein; stirring said suspension; and detecting a reduction in electrophoretic mobility of said particles.
Claim 7
Independent Claim
62 words
The method of detecting a reaction between a first protein and a second protein comprising the steps of: depositing said first protein on each of a plurality of microscopic particles; forming a dilute suspension of said particles in a solution to be tested for presence of said second protein; stirring said suspension; and detecting a reduction in electrophoretic mobility of said particles.
Claim 8
Dependent Claim
19 words
The method of claim 5 wherein the step of stirring said suspension comprises mixing said solution by electrophoretic motion.
Claim 9
Dependent Claim
21 words
The method of claim 5 wherein the step of stirring said suspension comprises mixing said solution turbulently and by electrophoretic motion.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-10-05 | 1980-04-05 | 1980-10-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-10-05 | 1984-04-05 | 1984-10-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-10-05 | 1988-04-05 | 1988-10-05 | $7700.00 | Window Closed / Lapsed |