US3855094A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method in the quantitative and qualitative determination of molecules having antigenic properties by immunoelectrophoresis
Assignee: Orion Oyj
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Filing: 1972-09-19
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Granted: 1974-12-17
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Expires: 1992-09-19
Abstract
Abstract In immunoelectrophoresis a method of determining the presence of molecules having antigenic properties and such electrochemical characteristics that their net mobility does not appreciably deviate from the antigens directed against the antibodies or do not have sufficient net mobility to be determined satisfactorily by conventional methods. Determination of the molecules is effected by adding certain specified compounds to the test sample prior to the electrophoresis.
Inventors
- 👤 A Teppo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:32
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; G01N27/44756
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
96 words
AN IMMUNOELECTROPHORETIC METTHOD OF QUANTITATIVELY AND QUALITATIVELY DETERRMINING THE PRESENCE IN A GEL MATRIX OF MOLECULES WHICH POSSESS ANTIGENIC PROPERTIES AND CONTAIN FREE AMINO GROUPS, COMPRISING: PROVIDING A GEL MATRIX CONTAINING MOLECULES WHOSE PROPERTIES ARE TO BE DETERMINED, SAID MAXTRIC COMPRISING AN ENVIRONMENT WHICH IS STABILIZED AGAINST UNCONTROLLED LIQUID CONVECTIONN IN SPACE, THE ELECTROCHEMICAL PROPERTIES OF SAID MOLEUCLES BEING SUCH THAT THEIR NET MOBILITY DOES NOT APPRECIABLE DEVIATE FROM THE NET MOBILITY OF ANTIGENS DIRECTED AGAINST ANTIBODIES IN SAID MATRIX, ADDING TO SAID GEL MATRIX CONTAINING SAID MOLECULES A COMPOUND SELECTED FROM THE GROUP CONSISTING OF:
Claim 2
Dependent Claim
96 words
AN IMMUNOELECTROPHORETIC METTHOD OF QUANTITATIVELY AND QUALITATIVELY DETERRMINING THE PRESENCE IN A GEL MATRIX OF MOLECULES WHICH POSSESS ANTIGENIC PROPERTIES AND CONTAIN FREE AMINO GROUPS, COMPRISING: PROVIDING A GEL MATRIX CONTAINING MOLECULES WHOSE PROPERTIES ARE TO BE DETERMINED, SAID MAXTRIC COMPRISING AN ENVIRONMENT WHICH IS STABILIZED AGAINST UNCONTROLLED LIQUID CONVECTIONN IN SPACE, THE ELECTROCHEMICAL PROPERTIES OF SAID MOLEUCLES BEING SUCH THAT THEIR NET MOBILITY DOES NOT APPRECIABLE DEVIATE FROM THE NET MOBILITY OF ANTIGENS DIRECTED AGAINST ANTIBODIES IN SAID MATRIX, ADDING TO SAID GEL MATRIX CONTAINING SAID MOLECULES A COMPOUND SELECTED FROM THE GROUP CONSISTING OF:
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said compound is formaldehyde or paraformaldehyde.
Claim 4
Dependent Claim
17 words
The method of claim 1, wherein the pH of the matrix is maintained between 5 and 10.
Claim 5
Dependent Claim
17 words
The method of claim 3, wherein the pH of the matrix is maintained between 7.6 and 9.3.
Claim 6
Dependent Claim
39 words
The method of claim 1 wherein said matrix is provided by allowing a gel matrix sample to stand for at least 5 minutes at a temperature in the range of 0*-45*C before said molecules are added to said matrix.
Claim 7
Dependent Claim
18 words
The method of claim 2, wherein the concentration of said compound in the matrix is at least 0.05M.
Claim 8
Dependent Claim
60 words
The method of claim 1, wherein before said electric current is passed through said gel matrix, formaldehyde is added to said matrix to a concentration in the range of 0.5-1.5 M, while said matrix has a pH in the range of 7.9-8.9, and said matrix is allowed to stand for 15 minutes at a temperature in the range of 5*-30*C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-16 | 1978-06-17 | 1978-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-16 | 1982-06-17 | 1982-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-16 | 1986-06-17 | 1986-12-17 | $7700.00 | Window Closed / Lapsed |