Patent US3979274A successfully refreshed from Google Patents.
US3979274A
Expired / Lapsed
US
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Membrane for enzyme electrodes
Assignee: Yellow Springs Instrument Co Inc
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Filing: 1975-09-24
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Granted: 1976-09-07
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Expires: 1995-09-24
Abstract
Abstract A laminated membrane for use with an enzyme electrode without the need for use of a compensating electrode. The laminated membrane comprises a layer of essentially homogeneous material such as cellulose acetate or silicone rubber which will prevent passage of even low molecular weight interfering materials, an adhesive layer of the enzyme itself (with or without other materials that may be blended with it), and a layer of support film which will also prevent the passage of high molecular weight interfering materials.
Inventors
- 👤 David P. Newman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:33
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; Y; Y10; Y10S; Y10S435/00; Y10S435/817; Y10T; Y10T428/00; Y10T428/24; Y10T428/24942; Y10T428/2495
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
143 words
A polarographic cell structure for use in polarographic analysis of an unknown comprising an electrically insulating support means, and electrode means mounted in said support means, said electrode means including means defining an active exposed working face, wherein the improvement comprises: a laminated membrane of a total thickness of less than 25 microns positioned between said working face and said unknown, said membrane consisting essentially of a first layer of an essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate, and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
Claim 2
Dependent Claim
143 words
A polarographic cell structure for use in polarographic analysis of an unknown comprising an electrically insulating support means, and electrode means mounted in said support means, said electrode means including means defining an active exposed working face, wherein the improvement comprises: a laminated membrane of a total thickness of less than 25 microns positioned between said working face and said unknown, said membrane consisting essentially of a first layer of an essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate, and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
Claim 3
Dependent Claim
26 words
A polaragraphic cell structure as set forth in claim 1 wherein the essentially homogeneous material is cellulose acetate having a thickness of 0.5 to 1.0 micron.
Claim 4
Dependent Claim
26 words
A polarographic cell structure as set forth in claim 2 wherein said enzyme is selected from the group consisting of glucose oxidase, glactose oxidase, and uricase.
Claim 5
Dependent Claim
38 words
A polarographic cell structure as set forth in claim 3 wherein said second layer is a porous polycarbonate film having a thickness of approximately 5-7 microns and the overall thickness of said membrane is less than 10 microns.
Claim 6
Dependent Claim
97 words
A laminated membrane having a total thickness of less than 25 microns for use in a polarographic cell consisting essentially of a first layer of essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
Claim 7
Dependent Claim
97 words
A laminated membrane having a total thickness of less than 25 microns for use in a polarographic cell consisting essentially of a first layer of essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
Claim 8
Dependent Claim
25 words
A laminated membrane as set forth in claim 5 wherein the essentially homogeneous material is cellulose acetate having a thickness of 0.5 to 1.0 micron.
Claim 9
Dependent Claim
37 words
A laminated membrane as set forth in claim 6 wherein said second layer is a porous polycarbonate film having a thickness of approximately 5-7 microns, and the overall thickness of said membrane is less than 10 microns.
Claim 10
Dependent Claim
29 words
A laminated membrane as set forth in claim 7 further including an O-ring adhesively bonded to said second layer for purposes of attaching the membrane to a polarographic cell.
Claim 11
Dependent Claim
25 words
A laminated membrane a set forth in claim 7 wherein said enzyme is selected from the group consisting of uricase, glactose oxidase, and glucose oxidase.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-07 | 1980-03-08 | 1980-09-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-07 | 1984-03-08 | 1984-09-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-07 | 1988-03-08 | 1988-09-07 | $7700.00 | Window Closed / Lapsed |