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US3973002A
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US
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Antihemophilic factor
Assignee: ER Squibb and Sons LLC
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Filing: 1975-05-01
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Granted: 1976-08-03
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Expires: 1995-05-01
Abstract
Abstract A quick-dissolving antihemophilic factor preparation is obtained by adjusting the pH of buffer-extracted plasma cryoprecipitate to from about pH 6 to about pH 7.0 and cooling to a temperature of from about 2°C to about 20°C for from about 15 minutes to about 1 hour, in order to precipitate certain protein impurities, primarily fibrinogen.
Inventors
- 👤 James J. Hagan
- 👤 Charles Glaser
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:49
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/14; A61K9/19; C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
58 words
In a method for isolating antihemophilic factor of human blood plasma, the improvement comprising adjusting the pH of a solution of buffer-extracted plasma cryoprecipitate to from about 6.0 to about 7.0, and cooling the solution at a temperature of from about 2°C to about 20°C for from about 15 to about 60 minutes to cause precipitation of impurities.
Claim 2
Dependent Claim
58 words
In a method for isolating antihemophilic factor of human blood plasma, the improvement comprising adjusting the pH of a solution of buffer-extracted plasma cryoprecipitate to from about 6.0 to about 7.0, and cooling the solution at a temperature of from about 2°C to about 20°C for from about 15 to about 60 minutes to cause precipitation of impurities.
Claim 3
Dependent Claim
24 words
A method according to claim 1 wherein the solution is held at a temperature of about 15°C and the resulting precipitated impurities are removed.
Claim 4
Dependent Claim
130 words
In a method for preparing lyophilized antihemophilic factor of human blood plasma wherein thawed frozen human blood plasma is centrifuged at a temperature of about 0°C to remove a cryoprecipitate, the cryoprecipitate is macerated, the macerated cryoprecipitate is extracted with buffer, the suspension is centifuged and the precipitate is discarded to obtain a buffer extract, the pH of the extract is adjusted to about 7.0-7.2 and the extract is sterilized and lyophilized, the improvement comprising adjusting the pH of the buffer extract to from about pH 6.0 to about pH 7.0, and cooling to a temperature of from about 2°C to about 20°C to precipitate an inert fibrous solid, and removing the precipitate while maintaining the temperature of the extract at a temperature of from about 2°C to about 20°C.
Claim 5
Dependent Claim
130 words
In a method for preparing lyophilized antihemophilic factor of human blood plasma wherein thawed frozen human blood plasma is centrifuged at a temperature of about 0°C to remove a cryoprecipitate, the cryoprecipitate is macerated, the macerated cryoprecipitate is extracted with buffer, the suspension is centifuged and the precipitate is discarded to obtain a buffer extract, the pH of the extract is adjusted to about 7.0-7.2 and the extract is sterilized and lyophilized, the improvement comprising adjusting the pH of the buffer extract to from about pH 6.0 to about pH 7.0, and cooling to a temperature of from about 2°C to about 20°C to precipitate an inert fibrous solid, and removing the precipitate while maintaining the temperature of the extract at a temperature of from about 2°C to about 20°C.
Claim 6
Dependent Claim
24 words
A method according to claim 3 wherein the buffer extract is cooled to a temperature of about 15°C and the precipitated impurity is removed.
Claim 7
Dependent Claim
14 words
A method according to claim 3 wherein said buffer comprises Tris hydrochloride or sulfate.
Claim 8
Dependent Claim
13 words
A method according to claim 3 wherein said buffer comprises a citrate-dextrose buffer.
Claim 9
Dependent Claim
29 words
A method according to claim 3 further including the step of mixing the buffer extract, prior to adjusting the pH thereof to 6 - 7, with aluminum hydroxide slurry.
Claim 10
Dependent Claim
37 words
A method according to claim 7 further including the improvement of adding sodium citrate to the buffer extract after adjusting the pH thereof to between 6 and 7, and then about neutral to stabilize said buffer extract.
Claim 11
Dependent Claim
16 words
A lyophilized antihemophilic factor of human blood plasma prepared according to the method of claim 3.
Claim 12
Dependent Claim
16 words
A lyophilized antihemophilic factor of human blood plasma prepared according to the method of claim 8.
References Cited (Backward Citations)
US3803115A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-08-03 | 1980-02-02 | 1980-08-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-08-03 | 1984-02-02 | 1984-08-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-08-03 | 1988-02-02 | 1988-08-03 | $7700.00 | Window Closed / Lapsed |