Patent US2424193A successfully refreshed from Google Patents.
Patent US3910825A successfully refreshed from Google Patents.
US3910825A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Water-insoluble peptide materials
Assignee: Bayer AG
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Filing: 1973-03-28
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Granted: 1975-10-07
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Expires: 1993-03-28
Abstract
Abstract Peptide materials such as enzymes and enzyme inhibitors are rendered water-insoluble by binding the peptide materials to a cross-linked copolymer formed by copolymerizing the following units: (A) about 0.1 to 50 weight percent of at least one Alpha , Beta -monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms and (B) about 99.9 to 50 weight percent of at least one di- and/or poly(meth) acrylate of a diol and/or a polyol.
Inventors
- 👤 Fritz Huper
- 👤 Erich Rauenbusch
- 👤 Gunter Schmidt-Kastner
- 👤 Bruno Bomer
- 👤 Herbert Bartl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:08
CPC Classifications
C; C08; C08F; C08F8/00; C08F20/00; C08F20/02; C08F20/10; C08F20/20; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
62 words
A WATER-INSLOUBLE PREPARATION COMPRISING A PEPTIDE MATERIAL SELECTED FROM ENZYMES AND ENZYME INHIBITORS BOUND TO A CROSS-LINKED COPOLYMER COMPRISING THE FOLLOWING COPOLYMERIZED UNITS: A. 0.1 TO 50 WEIGHT PERCENT OF AT LEAST ONE A-B-OLEFINICALLY UNSATURATED DICARBOXYLIC ACID ANHYDRIDE HAVING 4 TO 9 CARBON ATOMS: B. 99.9 TO 50 WEIGHT PERCENT OF AT LEAST ONE DI-AND-OR POLY-(METH)ACRYLATE OF A DIOL AND/OR A POLYOL.
Claim 2
Dependent Claim
62 words
A WATER-INSLOUBLE PREPARATION COMPRISING A PEPTIDE MATERIAL SELECTED FROM ENZYMES AND ENZYME INHIBITORS BOUND TO A CROSS-LINKED COPOLYMER COMPRISING THE FOLLOWING COPOLYMERIZED UNITS: A. 0.1 TO 50 WEIGHT PERCENT OF AT LEAST ONE A-B-OLEFINICALLY UNSATURATED DICARBOXYLIC ACID ANHYDRIDE HAVING 4 TO 9 CARBON ATOMS: B. 99.9 TO 50 WEIGHT PERCENT OF AT LEAST ONE DI-AND-OR POLY-(METH)ACRYLATE OF A DIOL AND/OR A POLYOL.
Claim 3
Dependent Claim
41 words
A preparation according to claim 1 in which the copolymer also comprises polymerized units derived from 0.01 to 30 weight percent, based on the total monomer units of the copolymer of a cross-linking agent having at least two non-conjugated double bonds.
Claim 4
Dependent Claim
88 words
A preparation according to claim 1 in which the copolymerized units A of the copolymer are derived from at least one anhydride selected from maleic, itaconic and citraconic anhydrides, and the polymerized units B of the copolymer are units of at least one acrylate selected from di-(meth)acrylates of diols having 2 to 4 carbon atoms, reaction products of 1 mol of diols having 2 to 4 carbon atoms with 1 to 20 mols of at least one alkylene oxide with 2 to 4 carbon atoms, and trimethylolpropane trimethacrylate.
Claim 5
Dependent Claim
42 words
A preparation according to claim 1 in which the copolymerized units A of the copolymer are of maleic anhydride and the copolymerized units B of the copolymer are of a dimethacrylate of a polyethylene glycol having a molecular weight up to 1000.
Claim 6
Dependent Claim
14 words
A preparation according to claim 1 in which the peptide material is an enzyme.
Claim 7
Dependent Claim
12 words
A preparation according to claim 5 in which the enzyme is penicillinacylase.
Claim 8
Dependent Claim
15 words
A preparation according to claim 1 in which the peptide material is an enzyme inhibitor.
Claim 9
Dependent Claim
97 words
A process for the preparation of the composition of claim 1 which comprises reacting the following amounts of copolymerizable monomers: A. 0.1 to 50 weight percent of the said at least one anhydride and B. 99.9 to 50 weight percent of the said acrylate; whereby copolymerization is conducted by precipitation or bead polymerization in a diluent inert to anhydride groups at a temperature of about 20* to 200*C in the presence of a free-radical initiator and the copolymer thus produced is reacted in aqueous suspension with a solution of the peptide material to give the desired preparation.
Claim 10
Dependent Claim
43 words
A process according to claim 8 in which the process is carried out by precipitation polymerization in an organic solvent, the organic solvent being present in a proportion of 5 to 20 parts by weight of solvent per part of monomer by weight.
Claim 11
Dependent Claim
64 words
A process according to claim 8 in which the copolymerization is carried out by suspension polymerization in an organic medium, the monomers and initiator being dissolved in an organic solvent which is immiscible with paraffins and inert towards anhydride groups, and dispersed in a continuous phase comprising a paraffin hydrocarbon, the volume ratio of continuous phase to disperse phase being between 2:1 and 5:1.
Claim 12
Dependent Claim
22 words
A process according to claim 3 in which the peptide solution is an enzyme solution having a pH of 5.5 to 9.0.
Claim 13
Dependent Claim
22 words
A process according to claim 8 in which the copolymer is reacted with an aqueous penicillinacylase solution at pH 5.7 to 6.8.
Claim 14
Dependent Claim
22 words
A process according to claim 12 in which the salt content of the aqueous penicillinacylase solution is not more than 0.2 mol/liter.
Claim 15
Dependent Claim
40 words
A process for carrying out an enzyme-catalyzed conversion of a substrate comprising contacting a preparation of an enzyme bound to a crosslinked copolymer according to claim 8 with a substrate for the enzyme under conditions where the said conversion occurs.
Claim 16
Dependent Claim
15 words
A process according to claim 14 in which the said conversion is a hydrolytic splitting.
Claim 17
Dependent Claim
31 words
A process according to claim 15 in which the substrate is a penicillin, the enzyme is penicillinacylase, and the conversion is a hydrolytic splitting of the penicillin to give 6-aminopenicillanic acid.
Claim 18
Dependent Claim
32 words
A process according to claim 16 in which the penicillin is in the form of an aqueous solution containing 75,000 to 150,000 IU/ml of penicillin and having a pH of about 7.8.
Claim 19
Dependent Claim
12 words
A preparation according to claim 5 in which the enzyme is asparaginase.
Claim 20
Dependent Claim
12 words
A preparation according to claim 5 in which the enzyme is elastase.
Claim 21
Dependent Claim
12 words
A preparation according to claim 5 in which the enzyme is urease.
Claim 22
Dependent Claim
12 words
A preparation according to claim 5 in which the enzyme is trypsin.
Claim 23
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is penicillinacylase and the copolymer comprises tetraethylene glycol dimethacrylate and maleic anhydride.
Claim 24
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is penicillinacylase and the compolymer comprises ethylene glycol dimethacrylate and maleic anhydride.
Claim 25
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is asparaginase and the copolymer comprises ethylene glycol dimethacrylate and maleic anhydride.
Claim 26
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is elastase and the copolymer comprises diethylene glycol dimethacrylate and maleic anhydride.
Claim 27
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is urease and the copolymer comprises diethylene glycol dimethacrylate and maleic anhydride.
Claim 28
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is trypsin and the copolymer comprises tetraethylene glycol dimethacrylate and maleic anhydride.
Claim 29
Dependent Claim
23 words
A preparation according to claim 1 in which the peptide material is elastase and the copolymer comprises tetraethylene glycol dimethacrylate and maleic anhydride.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-06 | 1979-04-07 | 1979-10-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-06 | 1983-04-07 | 1983-10-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-06 | 1987-04-07 | 1987-10-07 | $7700.00 | Window Closed / Lapsed |